Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/205126 |
Resumo: | The sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), is one of the most relevant pests in sugarcane fields and has been successfully managed in certain regions of Brazil with the use of the parasitoid Cotesia flavipes Cameron, 1891 (Hymenoptera: Braconidae). Endoparasitoids regulate the behavior and physiology of their hosts by secreting bioactive proteins synthesized by venom glands, symbiotic viruses, teratocytes or by the developing larva in the host's hemocoel. Venom is injected into the host to aid parasitoid successful establishment in the first days of parasitism. Teratocytes are extraembryonic cells present in the parasitoid egg that dissociate from the serosa after the parasitoid hatches and disperse in the host insect's hemocoel. Several venom- and teratocytes-derived peptides have been identified in different parasitoid wasps and many remain uncharacterized or unexplored. Investigations of the identity of host regulation-related molecules from C. flavipes may result in the identification of proteins with biotechnological potential. The purpose of this research was to apply modern proteotranscriptomic approaches for the identification host regulation-related proteins from C. flavipes, highlighting peptides from teratocytes and venom. A plenty of peptides produced by teratocytes and venom glands was identified. Following, some of these peptides were selected and evaluated in terms of immunosuppressive and insecticidal potentials against D. saccharalis larvae. Results point out biological and biotechnological potential of these peptides that shed light on the comprehension of the physiology of parasitism as well for the development of new insecticidal molecules. |
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Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae)Peptídeos relacionados à regulação hospedeira do endoparasitoide Cotesia flavipes (Hymenoptera: Braconidae): identificação, funções imunosupresivas e potencial inseticida em Diatraea saccharalis (Lepidoptera: Crambidae)Biological controlProteomicsBiotechnologyTranscriptomicsParasitism physiologyControle biológicoProteômicaBiotecnologiaTranscritômicaFisiologia do parasitismoThe sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), is one of the most relevant pests in sugarcane fields and has been successfully managed in certain regions of Brazil with the use of the parasitoid Cotesia flavipes Cameron, 1891 (Hymenoptera: Braconidae). Endoparasitoids regulate the behavior and physiology of their hosts by secreting bioactive proteins synthesized by venom glands, symbiotic viruses, teratocytes or by the developing larva in the host's hemocoel. Venom is injected into the host to aid parasitoid successful establishment in the first days of parasitism. Teratocytes are extraembryonic cells present in the parasitoid egg that dissociate from the serosa after the parasitoid hatches and disperse in the host insect's hemocoel. Several venom- and teratocytes-derived peptides have been identified in different parasitoid wasps and many remain uncharacterized or unexplored. Investigations of the identity of host regulation-related molecules from C. flavipes may result in the identification of proteins with biotechnological potential. The purpose of this research was to apply modern proteotranscriptomic approaches for the identification host regulation-related proteins from C. flavipes, highlighting peptides from teratocytes and venom. A plenty of peptides produced by teratocytes and venom glands was identified. Following, some of these peptides were selected and evaluated in terms of immunosuppressive and insecticidal potentials against D. saccharalis larvae. Results point out biological and biotechnological potential of these peptides that shed light on the comprehension of the physiology of parasitism as well for the development of new insecticidal molecules.A broca-da-cana, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), é uma das principais pragas da cultura da cana-de-açúcar e tem sido manejada com sucesso em determinadas regiões do Brasil com o uso do parasitoide Cotesia flavipes Cameron, 1891 (Hymenoptera: Braconidae). Vespas endoparasitoides como C. flavipes são capazes de regular a fisiologia de seus hospedeiros por meio da secreção de proteínas bioativas sintetizadas por glândulas de veneno, vírus simbiontes, teratócitos e pela própria larva em desenvolvimento na hemocele do hospedeiro. O veneno dos parasitoides é injetado para auxiliar o estabelecimento do parasitoide no interior do hospedeiro nos primeiros dias após a oviposição. Teratócitos são células extraembrionárias que se dissociam da serosa após a eclosão do parasitoide e se dispersam na hemocele do hospedeiro durante o desenvolvimento do parasitoide. A atividade dos teratócitos influencia diretamente a fisiologia do hospedeiro por meio da secreção de peptídeos específicos. Muitos peptídeos do veneno e dos teratócitos de parasitoides foram identificados, mas muitos são não caracterizados e explorados. O objetivo deste estudo foi aplicar técnicas modernas de proteo-transcritômica para a identificação de proteínas produzidas durante o parasitismo de C. flavipes sobre D. saccharalis com destaque para proteínas produzidas pelos teratócitos e glândulas de veneno do parasitoide. Uma grande gama de proteínas e peptídeos expressos pelos teratócitos e glândula de veneno de C. flavipes foi identificada. Em seguida, alguns desses peptídeos foram selecionados e avaliados em termos imunossupressivos e inseticida sobre lagartas de D. saccharalis. Os resultados obtidos indicaram funções imunossupressoras e biotecnológicas úteis para auxiliar a compreensão da fisiologia do parasitismo bem como para o desenvolvimento de novas moléculas inseticidas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)001Universidade Estadual Paulista (Unesp)Rossi, Guilherme Duarte [UNESP]Universidade Estadual Paulista (Unesp)Pinto, Ciro Pedro Guidotti [UNESP]2021-06-24T20:06:12Z2021-06-24T20:06:12Z2021-05-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/11449/20512633004102037P9enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-06-05T14:52:05Zoai:repositorio.unesp.br:11449/205126Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:11:42.386890Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) Peptídeos relacionados à regulação hospedeira do endoparasitoide Cotesia flavipes (Hymenoptera: Braconidae): identificação, funções imunosupresivas e potencial inseticida em Diatraea saccharalis (Lepidoptera: Crambidae) |
title |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
spellingShingle |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) Pinto, Ciro Pedro Guidotti [UNESP] Biological control Proteomics Biotechnology Transcriptomics Parasitism physiology Controle biológico Proteômica Biotecnologia Transcritômica Fisiologia do parasitismo |
title_short |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
title_full |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
title_fullStr |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
title_full_unstemmed |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
title_sort |
Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae) |
author |
Pinto, Ciro Pedro Guidotti [UNESP] |
author_facet |
Pinto, Ciro Pedro Guidotti [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Rossi, Guilherme Duarte [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pinto, Ciro Pedro Guidotti [UNESP] |
dc.subject.por.fl_str_mv |
Biological control Proteomics Biotechnology Transcriptomics Parasitism physiology Controle biológico Proteômica Biotecnologia Transcritômica Fisiologia do parasitismo |
topic |
Biological control Proteomics Biotechnology Transcriptomics Parasitism physiology Controle biológico Proteômica Biotecnologia Transcritômica Fisiologia do parasitismo |
description |
The sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), is one of the most relevant pests in sugarcane fields and has been successfully managed in certain regions of Brazil with the use of the parasitoid Cotesia flavipes Cameron, 1891 (Hymenoptera: Braconidae). Endoparasitoids regulate the behavior and physiology of their hosts by secreting bioactive proteins synthesized by venom glands, symbiotic viruses, teratocytes or by the developing larva in the host's hemocoel. Venom is injected into the host to aid parasitoid successful establishment in the first days of parasitism. Teratocytes are extraembryonic cells present in the parasitoid egg that dissociate from the serosa after the parasitoid hatches and disperse in the host insect's hemocoel. Several venom- and teratocytes-derived peptides have been identified in different parasitoid wasps and many remain uncharacterized or unexplored. Investigations of the identity of host regulation-related molecules from C. flavipes may result in the identification of proteins with biotechnological potential. The purpose of this research was to apply modern proteotranscriptomic approaches for the identification host regulation-related proteins from C. flavipes, highlighting peptides from teratocytes and venom. A plenty of peptides produced by teratocytes and venom glands was identified. Following, some of these peptides were selected and evaluated in terms of immunosuppressive and insecticidal potentials against D. saccharalis larvae. Results point out biological and biotechnological potential of these peptides that shed light on the comprehension of the physiology of parasitism as well for the development of new insecticidal molecules. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-24T20:06:12Z 2021-06-24T20:06:12Z 2021-05-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11449/205126 33004102037P9 |
url |
http://hdl.handle.net/11449/205126 |
identifier_str_mv |
33004102037P9 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129402887733248 |