Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.

Detalhes bibliográficos
Autor(a) principal: Okamoto, Tetuo [UNESP]
Data de Publicação: 2000
Outros Autores: Kira, Marcos Eduardo [UNESP], Okamoto, Roberta [UNESP], Garcia Júnior, Idelmo Rangel [UNESP], Niccoli Filho, Walter Domingos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.forp.usp.br/bdj/bdj11(2)/t02112/t02112.html
http://hdl.handle.net/11449/66375
Resumo: As a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.
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spelling Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.azo compoundcollagencoloring agentdiagnostic agenteosinfluorescent dyeglycerolhematoxylinmethyl greenpreservativeallotransplantationanimalbone developmentbone matrixconnective tissuefibroblastfollow upmaleosteoblastosteolysispathologyphysiologyrattibiatissue preservationtransplantationtrichrome stainWistar ratAnimalsAzo CompoundsBone MatrixBone ResorptionCollagenColoring AgentsConnective TissueEosine Yellowish-(YS)FibroblastsFluorescent DyesFollow-Up StudiesGlycerolHematoxylinMaleMethyl GreenOsteoblastsOsteogenesisPreservatives, PharmaceuticalRatsRats, WistarTibiaTissue PreservationTransplantation, HomologousAs a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.Universidade Estadual Paulista (Unesp)Okamoto, Tetuo [UNESP]Kira, Marcos Eduardo [UNESP]Okamoto, Roberta [UNESP]Garcia Júnior, Idelmo Rangel [UNESP]Niccoli Filho, Walter Domingos [UNESP]2014-05-27T11:20:11Z2014-05-27T11:20:11Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article79-87http://www.forp.usp.br/bdj/bdj11(2)/t02112/t02112.htmlBrazilian dental journal, v. 11, n. 2, p. 79-87, 2000.0103-6440http://hdl.handle.net/11449/663752-s2.0-0034567599016995536396809715669282198280568843503367647381Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2021-10-23T12:10:56Zoai:repositorio.unesp.br:11449/66375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:53:47.789934Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
title Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
spellingShingle Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
Okamoto, Tetuo [UNESP]
azo compound
collagen
coloring agent
diagnostic agent
eosin
fluorescent dye
glycerol
hematoxylin
methyl green
preservative
allotransplantation
animal
bone development
bone matrix
connective tissue
fibroblast
follow up
male
osteoblast
osteolysis
pathology
physiology
rat
tibia
tissue preservation
transplantation
trichrome stain
Wistar rat
Animals
Azo Compounds
Bone Matrix
Bone Resorption
Collagen
Coloring Agents
Connective Tissue
Eosine Yellowish-(YS)
Fibroblasts
Fluorescent Dyes
Follow-Up Studies
Glycerol
Hematoxylin
Male
Methyl Green
Osteoblasts
Osteogenesis
Preservatives, Pharmaceutical
Rats
Rats, Wistar
Tibia
Tissue Preservation
Transplantation, Homologous
title_short Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
title_full Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
title_fullStr Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
title_full_unstemmed Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
title_sort Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
author Okamoto, Tetuo [UNESP]
author_facet Okamoto, Tetuo [UNESP]
Kira, Marcos Eduardo [UNESP]
Okamoto, Roberta [UNESP]
Garcia Júnior, Idelmo Rangel [UNESP]
Niccoli Filho, Walter Domingos [UNESP]
author_role author
author2 Kira, Marcos Eduardo [UNESP]
Okamoto, Roberta [UNESP]
Garcia Júnior, Idelmo Rangel [UNESP]
Niccoli Filho, Walter Domingos [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Okamoto, Tetuo [UNESP]
Kira, Marcos Eduardo [UNESP]
Okamoto, Roberta [UNESP]
Garcia Júnior, Idelmo Rangel [UNESP]
Niccoli Filho, Walter Domingos [UNESP]
dc.subject.por.fl_str_mv azo compound
collagen
coloring agent
diagnostic agent
eosin
fluorescent dye
glycerol
hematoxylin
methyl green
preservative
allotransplantation
animal
bone development
bone matrix
connective tissue
fibroblast
follow up
male
osteoblast
osteolysis
pathology
physiology
rat
tibia
tissue preservation
transplantation
trichrome stain
Wistar rat
Animals
Azo Compounds
Bone Matrix
Bone Resorption
Collagen
Coloring Agents
Connective Tissue
Eosine Yellowish-(YS)
Fibroblasts
Fluorescent Dyes
Follow-Up Studies
Glycerol
Hematoxylin
Male
Methyl Green
Osteoblasts
Osteogenesis
Preservatives, Pharmaceutical
Rats
Rats, Wistar
Tibia
Tissue Preservation
Transplantation, Homologous
topic azo compound
collagen
coloring agent
diagnostic agent
eosin
fluorescent dye
glycerol
hematoxylin
methyl green
preservative
allotransplantation
animal
bone development
bone matrix
connective tissue
fibroblast
follow up
male
osteoblast
osteolysis
pathology
physiology
rat
tibia
tissue preservation
transplantation
trichrome stain
Wistar rat
Animals
Azo Compounds
Bone Matrix
Bone Resorption
Collagen
Coloring Agents
Connective Tissue
Eosine Yellowish-(YS)
Fibroblasts
Fluorescent Dyes
Follow-Up Studies
Glycerol
Hematoxylin
Male
Methyl Green
Osteoblasts
Osteogenesis
Preservatives, Pharmaceutical
Rats
Rats, Wistar
Tibia
Tissue Preservation
Transplantation, Homologous
description As a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
2014-05-27T11:20:11Z
2014-05-27T11:20:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.forp.usp.br/bdj/bdj11(2)/t02112/t02112.html
Brazilian dental journal, v. 11, n. 2, p. 79-87, 2000.
0103-6440
http://hdl.handle.net/11449/66375
2-s2.0-0034567599
0169955363968097
1566928219828056
8843503367647381
url http://www.forp.usp.br/bdj/bdj11(2)/t02112/t02112.html
http://hdl.handle.net/11449/66375
identifier_str_mv Brazilian dental journal, v. 11, n. 2, p. 79-87, 2000.
0103-6440
2-s2.0-0034567599
0169955363968097
1566928219828056
8843503367647381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Dental Journal
0,476
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 79-87
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129137161797632

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