Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00449-021-02555-1 http://hdl.handle.net/11449/209310 |
Resumo: | The production of biocompounds through the cultivation of filamentous microorganisms is mainly affected by Oxygen Transfer Rate (OTR) and shear rate ((gamma)overdot(av)) conditions. Despite efforts have been made to evaluate the effect of operating variables (impeller speed, N; and airflow rate, phi(air)) on clavulanic acid production, no analysis regarding the effect of OTR and (gamma)overdot(av) was made. Then, the aim of this study was to evaluate the dissociated effect of physical phenomena such as oxygen transfer and shear rate in the production of clavulanic acid from Streptomyces clavuligerus using a stirred tank bioreactor. Streptomyces clavuligerus cultivations were performed at five different OTR and (gamma)overdot(av) conditions by manipulating the operating conditions (N, phi(air), and gas inlet composition). Cultivations performed at equal impeller speed (600 rpm, similar (gamma)overdot(av)) using oxygen enrichment, showed that CA productivity (Prod(CA)) was positively affected by OTR increase. Subsequently, the different shear conditions (achieved by varying the impeller speed) lead to an increase in CA production levels. Despite both OTR and shear rate positively enhanced CA productivity, (gamma)overdot(av) exhibited the highest impact: an increase of 145% in OTRinitial enhanced the clavulanic acid productivity of about 29%, while an increment in the shear rate of 134% raised the Prod(CA) in 53%. |
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Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerusClavulanic acidShear rateOxygen transferStirred tank bioreactorThe production of biocompounds through the cultivation of filamentous microorganisms is mainly affected by Oxygen Transfer Rate (OTR) and shear rate ((gamma)overdot(av)) conditions. Despite efforts have been made to evaluate the effect of operating variables (impeller speed, N; and airflow rate, phi(air)) on clavulanic acid production, no analysis regarding the effect of OTR and (gamma)overdot(av) was made. Then, the aim of this study was to evaluate the dissociated effect of physical phenomena such as oxygen transfer and shear rate in the production of clavulanic acid from Streptomyces clavuligerus using a stirred tank bioreactor. Streptomyces clavuligerus cultivations were performed at five different OTR and (gamma)overdot(av) conditions by manipulating the operating conditions (N, phi(air), and gas inlet composition). Cultivations performed at equal impeller speed (600 rpm, similar (gamma)overdot(av)) using oxygen enrichment, showed that CA productivity (Prod(CA)) was positively affected by OTR increase. Subsequently, the different shear conditions (achieved by varying the impeller speed) lead to an increase in CA production levels. Despite both OTR and shear rate positively enhanced CA productivity, (gamma)overdot(av) exhibited the highest impact: an increase of 145% in OTRinitial enhanced the clavulanic acid productivity of about 29%, while an increment in the shear rate of 134% raised the Prod(CA) in 53%.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Engn Bioproc, Campinas, SP, BrazilFed Inst Educ Sci & Technol Sao Paulo, Campus Capivari, BR-13360000 Capivari, SP, BrazilUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc Engn & Biotechnol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc Engn & Biotechnol, BR-14801902 Araraquara, SP, BrazilSpringerUniversidade Estadual de Campinas (UNICAMP)Fed Inst Educ Sci & Technol Sao PauloUniversidade Estadual Paulista (Unesp)Ribeiro, Renata M. M. G. P.Esperanca, Mateus N.Sousa, Ana P. A. [UNESP]Neto, Alvaro Baptista [UNESP]Cerri, Marcel O. [UNESP]2021-06-25T11:55:56Z2021-06-25T11:55:56Z2021-04-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1007/s00449-021-02555-1Bioprocess And Biosystems Engineering. New York: Springer, 12 p., 2021.1615-7591http://hdl.handle.net/11449/20931010.1007/s00449-021-02555-1WOS:000636943700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioprocess And Biosystems Engineeringinfo:eu-repo/semantics/openAccess2021-10-23T19:28:03Zoai:repositorio.unesp.br:11449/209310Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:36:12.607188Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
title |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
spellingShingle |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus Ribeiro, Renata M. M. G. P. Clavulanic acid Shear rate Oxygen transfer Stirred tank bioreactor |
title_short |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
title_full |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
title_fullStr |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
title_full_unstemmed |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
title_sort |
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus |
author |
Ribeiro, Renata M. M. G. P. |
author_facet |
Ribeiro, Renata M. M. G. P. Esperanca, Mateus N. Sousa, Ana P. A. [UNESP] Neto, Alvaro Baptista [UNESP] Cerri, Marcel O. [UNESP] |
author_role |
author |
author2 |
Esperanca, Mateus N. Sousa, Ana P. A. [UNESP] Neto, Alvaro Baptista [UNESP] Cerri, Marcel O. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Fed Inst Educ Sci & Technol Sao Paulo Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ribeiro, Renata M. M. G. P. Esperanca, Mateus N. Sousa, Ana P. A. [UNESP] Neto, Alvaro Baptista [UNESP] Cerri, Marcel O. [UNESP] |
dc.subject.por.fl_str_mv |
Clavulanic acid Shear rate Oxygen transfer Stirred tank bioreactor |
topic |
Clavulanic acid Shear rate Oxygen transfer Stirred tank bioreactor |
description |
The production of biocompounds through the cultivation of filamentous microorganisms is mainly affected by Oxygen Transfer Rate (OTR) and shear rate ((gamma)overdot(av)) conditions. Despite efforts have been made to evaluate the effect of operating variables (impeller speed, N; and airflow rate, phi(air)) on clavulanic acid production, no analysis regarding the effect of OTR and (gamma)overdot(av) was made. Then, the aim of this study was to evaluate the dissociated effect of physical phenomena such as oxygen transfer and shear rate in the production of clavulanic acid from Streptomyces clavuligerus using a stirred tank bioreactor. Streptomyces clavuligerus cultivations were performed at five different OTR and (gamma)overdot(av) conditions by manipulating the operating conditions (N, phi(air), and gas inlet composition). Cultivations performed at equal impeller speed (600 rpm, similar (gamma)overdot(av)) using oxygen enrichment, showed that CA productivity (Prod(CA)) was positively affected by OTR increase. Subsequently, the different shear conditions (achieved by varying the impeller speed) lead to an increase in CA production levels. Despite both OTR and shear rate positively enhanced CA productivity, (gamma)overdot(av) exhibited the highest impact: an increase of 145% in OTRinitial enhanced the clavulanic acid productivity of about 29%, while an increment in the shear rate of 134% raised the Prod(CA) in 53%. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:55:56Z 2021-06-25T11:55:56Z 2021-04-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00449-021-02555-1 Bioprocess And Biosystems Engineering. New York: Springer, 12 p., 2021. 1615-7591 http://hdl.handle.net/11449/209310 10.1007/s00449-021-02555-1 WOS:000636943700001 |
url |
http://dx.doi.org/10.1007/s00449-021-02555-1 http://hdl.handle.net/11449/209310 |
identifier_str_mv |
Bioprocess And Biosystems Engineering. New York: Springer, 12 p., 2021. 1615-7591 10.1007/s00449-021-02555-1 WOS:000636943700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioprocess And Biosystems Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128385683030016 |