Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5588/ijtld.11.0658 http://hdl.handle.net/11449/42034 |
Resumo: | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity buildingMycobacterium tuberculosisrpoBsputum smear microscopysodium hypochloritedenaturing high-performance liquid chromatography (DHPLC) sequencingThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.Luso-American Development Foundation (LACR Award program)Inst Nacl Saúde Dr Ricardo Jorge INSA, IP, P-1649016 Lisbon, PortugalCumura Hosp, Cumura, Guinea BissauHosp Fernando Fonseca, Serv Patol Clin, Amadora, PortugalUniv Evora, Inst Ciencias Agrarias & Ambientais Mediterran, Escola Ciencias & Tecnol, Evora, PortugalUniv Estadual Paulista, Fac Pharmaceut Sci, São Paulo, BrazilInst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, BrazilUniv Nova Lisboa, IHMT, P-1200 Lisbon, PortugalUniv Estadual Paulista, Fac Pharmaceut Sci, São Paulo, BrazilInt Union Against Tuberculosis Lung Disease (i U A T L D)Inst Nacl Saúde Dr Ricardo Jorge INSACumura HospHosp Fernando FonsecaUniv EvoraUniversidade Estadual Paulista (Unesp)Inst Nacl de Pesquisas da AmazoniaUniv Nova LisboaDavid, S.Sutre, A. F.Sanca, A.Mane, A.Henriques, V.Portugal, C.Sancho, L.Cardoso, A.Paixao, E.Duarte, E. L.Leite, Clarice Queico Fujimura [UNESP]Salem, J. I.Antunes, A.2014-05-20T15:33:24Z2014-05-20T15:33:24Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1535-1537http://dx.doi.org/10.5588/ijtld.11.0658International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.1027-3719http://hdl.handle.net/11449/4203410.5588/ijtld.11.0658WOS:0003101739000202114570774349859Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Tuberculosis and Lung Disease2.3921,533info:eu-repo/semantics/openAccess2024-06-24T13:06:54Zoai:repositorio.unesp.br:11449/42034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:14.082743Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
title |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
spellingShingle |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building David, S. Mycobacterium tuberculosis rpoB sputum smear microscopy sodium hypochlorite denaturing high-performance liquid chromatography (DHPLC) sequencing |
title_short |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
title_full |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
title_fullStr |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
title_full_unstemmed |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
title_sort |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
author |
David, S. |
author_facet |
David, S. Sutre, A. F. Sanca, A. Mane, A. Henriques, V. Portugal, C. Sancho, L. Cardoso, A. Paixao, E. Duarte, E. L. Leite, Clarice Queico Fujimura [UNESP] Salem, J. I. Antunes, A. |
author_role |
author |
author2 |
Sutre, A. F. Sanca, A. Mane, A. Henriques, V. Portugal, C. Sancho, L. Cardoso, A. Paixao, E. Duarte, E. L. Leite, Clarice Queico Fujimura [UNESP] Salem, J. I. Antunes, A. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Inst Nacl Saúde Dr Ricardo Jorge INSA Cumura Hosp Hosp Fernando Fonseca Univ Evora Universidade Estadual Paulista (Unesp) Inst Nacl de Pesquisas da Amazonia Univ Nova Lisboa |
dc.contributor.author.fl_str_mv |
David, S. Sutre, A. F. Sanca, A. Mane, A. Henriques, V. Portugal, C. Sancho, L. Cardoso, A. Paixao, E. Duarte, E. L. Leite, Clarice Queico Fujimura [UNESP] Salem, J. I. Antunes, A. |
dc.subject.por.fl_str_mv |
Mycobacterium tuberculosis rpoB sputum smear microscopy sodium hypochlorite denaturing high-performance liquid chromatography (DHPLC) sequencing |
topic |
Mycobacterium tuberculosis rpoB sputum smear microscopy sodium hypochlorite denaturing high-performance liquid chromatography (DHPLC) sequencing |
description |
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2014-05-20T15:33:24Z 2014-05-20T15:33:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5588/ijtld.11.0658 International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012. 1027-3719 http://hdl.handle.net/11449/42034 10.5588/ijtld.11.0658 WOS:000310173900020 2114570774349859 |
url |
http://dx.doi.org/10.5588/ijtld.11.0658 http://hdl.handle.net/11449/42034 |
identifier_str_mv |
International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012. 1027-3719 10.5588/ijtld.11.0658 WOS:000310173900020 2114570774349859 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Tuberculosis and Lung Disease 2.392 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1535-1537 |
dc.publisher.none.fl_str_mv |
Int Union Against Tuberculosis Lung Disease (i U A T L D) |
publisher.none.fl_str_mv |
Int Union Against Tuberculosis Lung Disease (i U A T L D) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128366757281792 |