Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building

Detalhes bibliográficos
Autor(a) principal: David, S.
Data de Publicação: 2012
Outros Autores: Sutre, A. F., Sanca, A., Mane, A., Henriques, V., Portugal, C., Sancho, L., Cardoso, A., Paixao, E., Duarte, E. L., Leite, Clarice Queico Fujimura [UNESP], Salem, J. I., Antunes, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5588/ijtld.11.0658
http://hdl.handle.net/11449/42034
Resumo: The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
id UNSP_a27e39aa9c715f5c5c47e9b5b4e3c58d
oai_identifier_str oai:repositorio.unesp.br:11449/42034
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity buildingMycobacterium tuberculosisrpoBsputum smear microscopysodium hypochloritedenaturing high-performance liquid chromatography (DHPLC) sequencingThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.Luso-American Development Foundation (LACR Award program)Inst Nacl Saúde Dr Ricardo Jorge INSA, IP, P-1649016 Lisbon, PortugalCumura Hosp, Cumura, Guinea BissauHosp Fernando Fonseca, Serv Patol Clin, Amadora, PortugalUniv Evora, Inst Ciencias Agrarias & Ambientais Mediterran, Escola Ciencias & Tecnol, Evora, PortugalUniv Estadual Paulista, Fac Pharmaceut Sci, São Paulo, BrazilInst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, BrazilUniv Nova Lisboa, IHMT, P-1200 Lisbon, PortugalUniv Estadual Paulista, Fac Pharmaceut Sci, São Paulo, BrazilInt Union Against Tuberculosis Lung Disease (i U A T L D)Inst Nacl Saúde Dr Ricardo Jorge INSACumura HospHosp Fernando FonsecaUniv EvoraUniversidade Estadual Paulista (Unesp)Inst Nacl de Pesquisas da AmazoniaUniv Nova LisboaDavid, S.Sutre, A. F.Sanca, A.Mane, A.Henriques, V.Portugal, C.Sancho, L.Cardoso, A.Paixao, E.Duarte, E. L.Leite, Clarice Queico Fujimura [UNESP]Salem, J. I.Antunes, A.2014-05-20T15:33:24Z2014-05-20T15:33:24Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1535-1537http://dx.doi.org/10.5588/ijtld.11.0658International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.1027-3719http://hdl.handle.net/11449/4203410.5588/ijtld.11.0658WOS:0003101739000202114570774349859Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Tuberculosis and Lung Disease2.3921,533info:eu-repo/semantics/openAccess2024-06-24T13:06:54Zoai:repositorio.unesp.br:11449/42034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:14.082743Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
spellingShingle Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
David, S.
Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
denaturing high-performance liquid chromatography (DHPLC) sequencing
title_short Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_full Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_fullStr Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_full_unstemmed Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_sort Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
author David, S.
author_facet David, S.
Sutre, A. F.
Sanca, A.
Mane, A.
Henriques, V.
Portugal, C.
Sancho, L.
Cardoso, A.
Paixao, E.
Duarte, E. L.
Leite, Clarice Queico Fujimura [UNESP]
Salem, J. I.
Antunes, A.
author_role author
author2 Sutre, A. F.
Sanca, A.
Mane, A.
Henriques, V.
Portugal, C.
Sancho, L.
Cardoso, A.
Paixao, E.
Duarte, E. L.
Leite, Clarice Queico Fujimura [UNESP]
Salem, J. I.
Antunes, A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Inst Nacl Saúde Dr Ricardo Jorge INSA
Cumura Hosp
Hosp Fernando Fonseca
Univ Evora
Universidade Estadual Paulista (Unesp)
Inst Nacl de Pesquisas da Amazonia
Univ Nova Lisboa
dc.contributor.author.fl_str_mv David, S.
Sutre, A. F.
Sanca, A.
Mane, A.
Henriques, V.
Portugal, C.
Sancho, L.
Cardoso, A.
Paixao, E.
Duarte, E. L.
Leite, Clarice Queico Fujimura [UNESP]
Salem, J. I.
Antunes, A.
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
denaturing high-performance liquid chromatography (DHPLC) sequencing
topic Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
denaturing high-performance liquid chromatography (DHPLC) sequencing
description The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
2014-05-20T15:33:24Z
2014-05-20T15:33:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5588/ijtld.11.0658
International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.
1027-3719
http://hdl.handle.net/11449/42034
10.5588/ijtld.11.0658
WOS:000310173900020
2114570774349859
url http://dx.doi.org/10.5588/ijtld.11.0658
http://hdl.handle.net/11449/42034
identifier_str_mv International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.
1027-3719
10.5588/ijtld.11.0658
WOS:000310173900020
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Tuberculosis and Lung Disease
2.392
1,533
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1535-1537
dc.publisher.none.fl_str_mv Int Union Against Tuberculosis Lung Disease (i U A T L D)
publisher.none.fl_str_mv Int Union Against Tuberculosis Lung Disease (i U A T L D)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128366757281792

Registros relacionados