Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-4689zool-20150192 http://hdl.handle.net/11449/165270 |
Resumo: | Secretory cells of the cephalic salivary glands (CSGs) of eusocial bees produce and accumulate lipid-like secretion in the lumens of their alveoli. Correspondingly, secretory cells present typical ultrastructural features of lipid-compound producers. Previous work on bees has revealed inter-specific differences in the chemical composition of secretion, and the production mechanisms and secretory cycle of secretory cells. In this work a comparative analysis of the mechanisms of lipid storage in the CSGs of Apis mellifera (Linnaeus, 1758) and Scaptotrigona postica (Latreille, 1807) workers was carried out. The ultrastructural location of lipids was ascertained using imidazole-osmium (IO), using individuals in different stages of their life cycles. Lipid deposits were identified inside glandular cells and in the alveolar lumens in all individuals, but differences were observed between the species. The glandular cells of A. mellifera workers presented positive reactions to IO as droplets dispersed in the cytoplasm, as vesicles and in the channels formed by apical plasma membrane infolds. In S. postica, lipid compounds were detected inside the mitochondrial matrix and in smooth endoplasmic reticulum cisterns. In both species, forager workers exhibited the largest amounts of lipids stored in the alveolar lumen. The differences between the species are discussed, taking into account specific behavioral differences. |
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Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workersCytochemistryexcretoryimidazole-osmiuminsectsalivary systemSecretory cells of the cephalic salivary glands (CSGs) of eusocial bees produce and accumulate lipid-like secretion in the lumens of their alveoli. Correspondingly, secretory cells present typical ultrastructural features of lipid-compound producers. Previous work on bees has revealed inter-specific differences in the chemical composition of secretion, and the production mechanisms and secretory cycle of secretory cells. In this work a comparative analysis of the mechanisms of lipid storage in the CSGs of Apis mellifera (Linnaeus, 1758) and Scaptotrigona postica (Latreille, 1807) workers was carried out. The ultrastructural location of lipids was ascertained using imidazole-osmium (IO), using individuals in different stages of their life cycles. Lipid deposits were identified inside glandular cells and in the alveolar lumens in all individuals, but differences were observed between the species. The glandular cells of A. mellifera workers presented positive reactions to IO as droplets dispersed in the cytoplasm, as vesicles and in the channels formed by apical plasma membrane infolds. In S. postica, lipid compounds were detected inside the mitochondrial matrix and in smooth endoplasmic reticulum cisterns. In both species, forager workers exhibited the largest amounts of lipids stored in the alveolar lumen. The differences between the species are discussed, taking into account specific behavioral differences.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Inst Biociencias, Ave 24A,1515 Bela Vista, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Ave 24A,1515 Bela Vista, BR-13506900 Rio Claro, SP, BrazilFAPESP: 07/56682-1Soc Brasileira Zoologia, Univ Federal ParanaUniversidade Estadual Paulista (Unesp)Poiani, Silvana Beani [UNESP]Cruz-Landim, Carminda da [UNESP]2018-11-27T19:23:17Z2018-11-27T19:23:17Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1590/S1984-4689zool-20150192Zoologia. Curitiba: Soc Brasileira Zoologia, Univ Federal Parana, v. 33, n. 3, 7 p., 2016.1984-4689http://hdl.handle.net/11449/16527010.1590/S1984-4689zool-20150192S1984-46702016000300901WOS:000380851100004S1984-46702016000300901.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZoologiainfo:eu-repo/semantics/openAccess2023-12-05T06:15:40Zoai:repositorio.unesp.br:11449/165270Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:31:39.415953Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
title |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
spellingShingle |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers Poiani, Silvana Beani [UNESP] Cytochemistry excretory imidazole-osmium insect salivary system |
title_short |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
title_full |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
title_fullStr |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
title_full_unstemmed |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
title_sort |
Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers |
author |
Poiani, Silvana Beani [UNESP] |
author_facet |
Poiani, Silvana Beani [UNESP] Cruz-Landim, Carminda da [UNESP] |
author_role |
author |
author2 |
Cruz-Landim, Carminda da [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Poiani, Silvana Beani [UNESP] Cruz-Landim, Carminda da [UNESP] |
dc.subject.por.fl_str_mv |
Cytochemistry excretory imidazole-osmium insect salivary system |
topic |
Cytochemistry excretory imidazole-osmium insect salivary system |
description |
Secretory cells of the cephalic salivary glands (CSGs) of eusocial bees produce and accumulate lipid-like secretion in the lumens of their alveoli. Correspondingly, secretory cells present typical ultrastructural features of lipid-compound producers. Previous work on bees has revealed inter-specific differences in the chemical composition of secretion, and the production mechanisms and secretory cycle of secretory cells. In this work a comparative analysis of the mechanisms of lipid storage in the CSGs of Apis mellifera (Linnaeus, 1758) and Scaptotrigona postica (Latreille, 1807) workers was carried out. The ultrastructural location of lipids was ascertained using imidazole-osmium (IO), using individuals in different stages of their life cycles. Lipid deposits were identified inside glandular cells and in the alveolar lumens in all individuals, but differences were observed between the species. The glandular cells of A. mellifera workers presented positive reactions to IO as droplets dispersed in the cytoplasm, as vesicles and in the channels formed by apical plasma membrane infolds. In S. postica, lipid compounds were detected inside the mitochondrial matrix and in smooth endoplasmic reticulum cisterns. In both species, forager workers exhibited the largest amounts of lipids stored in the alveolar lumen. The differences between the species are discussed, taking into account specific behavioral differences. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-11-27T19:23:17Z 2018-11-27T19:23:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-4689zool-20150192 Zoologia. Curitiba: Soc Brasileira Zoologia, Univ Federal Parana, v. 33, n. 3, 7 p., 2016. 1984-4689 http://hdl.handle.net/11449/165270 10.1590/S1984-4689zool-20150192 S1984-46702016000300901 WOS:000380851100004 S1984-46702016000300901.pdf |
url |
http://dx.doi.org/10.1590/S1984-4689zool-20150192 http://hdl.handle.net/11449/165270 |
identifier_str_mv |
Zoologia. Curitiba: Soc Brasileira Zoologia, Univ Federal Parana, v. 33, n. 3, 7 p., 2016. 1984-4689 10.1590/S1984-4689zool-20150192 S1984-46702016000300901 WOS:000380851100004 S1984-46702016000300901.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zoologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Zoologia, Univ Federal Parana |
publisher.none.fl_str_mv |
Soc Brasileira Zoologia, Univ Federal Parana |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129081377554432 |