Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3168/jds.2019-17772 http://hdl.handle.net/11449/196918 |
Resumo: | The aim of this study was to determine the association between estrous expression, measured using a breeding indicator and an automated activity monitor (AAM), and the success of embryo collection after superovulation. Holstein heifers (n = 51; 10.5 to 14.5 mo, and 325.0 +/- 21.1 kg of body weight) were superovulated (n = 69 events) for the collection of embryos using a protocol based on sequential administration of FSH for follicle superstimulation and GnRH to induce ovulation. Artificial insemination (AI) was performed twice, once at the moment of GnRH administration and again 12 h later, using thawed, sexed semen. Ovaries were scanned via ultrasonography on the day of the first AI to count the total number of preovulatory follicles and 7 d later for the total number of corpora lutea present. Embryos were collected 7 d post-AI, counted, and assessed for viability. A breeding indicator (Estrotect, Rockway Inc., Spring Valley, WI) and a collar-mounted AAM (CowScout Activity Monitoring System, GEA, Dusseldorf, Germany) were used to measure standing mounts and an algorithmic estimate of estrous expression, respectively. A score for the breeding indicator was given as follows: score 1 = 100% of the indicator was intact; score 2 = 50% of the indicator was rubbed off; score 3 = greater than 50% of the indicator was rubbed off. Estrous expression detected by the AAM was quantified through the relative increase in physical activity and duration of time spent above a set threshold. Data were analyzed by ANOVA using the MIXED procedures of SAS (SAS Institute Inc., Cary, NC). The number of follicles present at AI was not affected by estrous expression. The mean (+/- SD) ovulatory response was 67.5 +/- 26.3%. We found an effect of estrous expression as detected by the breeding indicator on the ovulatory response (42.1 +/- 8.0, vs. 78.2 +/- 9.0, vs. 74.0 +/- 4.9%, for scores 1, 2, and 3, respectively) but not from the AAM. Heifers that had a score of 3 (versus those with scores of 1 and 2) on the breeding indicator had a greater number of embryos (4.1 +/- 0.5, vs. 1.2 +/- 1.0, vs. 1.8 +/- 1.0 embryos), and a greater percentage of these embryos were viable (43.1 +/- 0.05, vs. 35.5 +/- 0.1, vs. 34.3 +/- 0.1%). Similarly, heifers that showed a greater intensity of activity (as measured by the AAM) had a greater number of embryos collected (10.2 +/- 1.2 vs. 6.0 +/- 1.3 embryos), and a greater percentage of those embryos were viable (53.1 +/- 5.0 vs. 23.4 +/- 5.1%). Longer-duration estrus episodes were associated with a higher percentage of viable embryos (51.2 +/- 5.2 vs. 25.3 +/- 5.3%). In conclusion, stronger estrous intensity was associated with a greater number of total embryos collected and a greater percentage of viable embryos. These results suggest that monitoring the intensity of estrus could be used to predict superovulatory response as well as embryo quality in Holstein heifers. |
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Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifersembryo transferestrous expressionautomated activity monitorThe aim of this study was to determine the association between estrous expression, measured using a breeding indicator and an automated activity monitor (AAM), and the success of embryo collection after superovulation. Holstein heifers (n = 51; 10.5 to 14.5 mo, and 325.0 +/- 21.1 kg of body weight) were superovulated (n = 69 events) for the collection of embryos using a protocol based on sequential administration of FSH for follicle superstimulation and GnRH to induce ovulation. Artificial insemination (AI) was performed twice, once at the moment of GnRH administration and again 12 h later, using thawed, sexed semen. Ovaries were scanned via ultrasonography on the day of the first AI to count the total number of preovulatory follicles and 7 d later for the total number of corpora lutea present. Embryos were collected 7 d post-AI, counted, and assessed for viability. A breeding indicator (Estrotect, Rockway Inc., Spring Valley, WI) and a collar-mounted AAM (CowScout Activity Monitoring System, GEA, Dusseldorf, Germany) were used to measure standing mounts and an algorithmic estimate of estrous expression, respectively. A score for the breeding indicator was given as follows: score 1 = 100% of the indicator was intact; score 2 = 50% of the indicator was rubbed off; score 3 = greater than 50% of the indicator was rubbed off. Estrous expression detected by the AAM was quantified through the relative increase in physical activity and duration of time spent above a set threshold. Data were analyzed by ANOVA using the MIXED procedures of SAS (SAS Institute Inc., Cary, NC). The number of follicles present at AI was not affected by estrous expression. The mean (+/- SD) ovulatory response was 67.5 +/- 26.3%. We found an effect of estrous expression as detected by the breeding indicator on the ovulatory response (42.1 +/- 8.0, vs. 78.2 +/- 9.0, vs. 74.0 +/- 4.9%, for scores 1, 2, and 3, respectively) but not from the AAM. Heifers that had a score of 3 (versus those with scores of 1 and 2) on the breeding indicator had a greater number of embryos (4.1 +/- 0.5, vs. 1.2 +/- 1.0, vs. 1.8 +/- 1.0 embryos), and a greater percentage of these embryos were viable (43.1 +/- 0.05, vs. 35.5 +/- 0.1, vs. 34.3 +/- 0.1%). Similarly, heifers that showed a greater intensity of activity (as measured by the AAM) had a greater number of embryos collected (10.2 +/- 1.2 vs. 6.0 +/- 1.3 embryos), and a greater percentage of those embryos were viable (53.1 +/- 5.0 vs. 23.4 +/- 5.1%). Longer-duration estrus episodes were associated with a higher percentage of viable embryos (51.2 +/- 5.2 vs. 25.3 +/- 5.3%). In conclusion, stronger estrous intensity was associated with a greater number of total embryos collected and a greater percentage of viable embryos. These results suggest that monitoring the intensity of estrus could be used to predict superovulatory response as well as embryo quality in Holstein heifers.Dairy Research Cluster Initiative II (Dairy Farmers of Canada)Agriculture and Agri-Food CanadaCanadian Dairy NetworkCanadian Dairy Commission; Ottawa, CanadaNatural Sciences and Engineering Research Council, Discovery Grant (Ottawa, Canada)Univ British Columbia, Fac Land & Food Syst, Appl Anim Biol, Vancouver, BC V6T 1Z4, CanadaTexas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USASao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, BrazilSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, BrazilElsevier B.V.Univ British ColumbiaTexas A&M UnivUniversidade Estadual Paulista (Unesp)Madureira, A. M. L.Burnett, T. A.Pohler, K. G.Guida, T. G. [UNESP]Sanches Jr, C. P. [UNESP]Vasconcelos, J. L. M. [UNESP]Cerri, R. L. A.2020-12-10T20:00:23Z2020-12-10T20:00:23Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5641-5646http://dx.doi.org/10.3168/jds.2019-17772Journal Of Dairy Science. New York: Elsevier Science Inc, v. 103, n. 6, p. 5641-5646, 2020.0022-0302http://hdl.handle.net/11449/19691810.3168/jds.2019-17772WOS:000535919900032Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Dairy Scienceinfo:eu-repo/semantics/openAccess2024-09-09T13:00:18Zoai:repositorio.unesp.br:11449/196918Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:00:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
title |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
spellingShingle |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers Madureira, A. M. L. embryo transfer estrous expression automated activity monitor |
title_short |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
title_full |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
title_fullStr |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
title_full_unstemmed |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
title_sort |
Short communication: Greater intensity of estrous expression is associated with improved embryo viability from superovulated Holstein heifers |
author |
Madureira, A. M. L. |
author_facet |
Madureira, A. M. L. Burnett, T. A. Pohler, K. G. Guida, T. G. [UNESP] Sanches Jr, C. P. [UNESP] Vasconcelos, J. L. M. [UNESP] Cerri, R. L. A. |
author_role |
author |
author2 |
Burnett, T. A. Pohler, K. G. Guida, T. G. [UNESP] Sanches Jr, C. P. [UNESP] Vasconcelos, J. L. M. [UNESP] Cerri, R. L. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ British Columbia Texas A&M Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Madureira, A. M. L. Burnett, T. A. Pohler, K. G. Guida, T. G. [UNESP] Sanches Jr, C. P. [UNESP] Vasconcelos, J. L. M. [UNESP] Cerri, R. L. A. |
dc.subject.por.fl_str_mv |
embryo transfer estrous expression automated activity monitor |
topic |
embryo transfer estrous expression automated activity monitor |
description |
The aim of this study was to determine the association between estrous expression, measured using a breeding indicator and an automated activity monitor (AAM), and the success of embryo collection after superovulation. Holstein heifers (n = 51; 10.5 to 14.5 mo, and 325.0 +/- 21.1 kg of body weight) were superovulated (n = 69 events) for the collection of embryos using a protocol based on sequential administration of FSH for follicle superstimulation and GnRH to induce ovulation. Artificial insemination (AI) was performed twice, once at the moment of GnRH administration and again 12 h later, using thawed, sexed semen. Ovaries were scanned via ultrasonography on the day of the first AI to count the total number of preovulatory follicles and 7 d later for the total number of corpora lutea present. Embryos were collected 7 d post-AI, counted, and assessed for viability. A breeding indicator (Estrotect, Rockway Inc., Spring Valley, WI) and a collar-mounted AAM (CowScout Activity Monitoring System, GEA, Dusseldorf, Germany) were used to measure standing mounts and an algorithmic estimate of estrous expression, respectively. A score for the breeding indicator was given as follows: score 1 = 100% of the indicator was intact; score 2 = 50% of the indicator was rubbed off; score 3 = greater than 50% of the indicator was rubbed off. Estrous expression detected by the AAM was quantified through the relative increase in physical activity and duration of time spent above a set threshold. Data were analyzed by ANOVA using the MIXED procedures of SAS (SAS Institute Inc., Cary, NC). The number of follicles present at AI was not affected by estrous expression. The mean (+/- SD) ovulatory response was 67.5 +/- 26.3%. We found an effect of estrous expression as detected by the breeding indicator on the ovulatory response (42.1 +/- 8.0, vs. 78.2 +/- 9.0, vs. 74.0 +/- 4.9%, for scores 1, 2, and 3, respectively) but not from the AAM. Heifers that had a score of 3 (versus those with scores of 1 and 2) on the breeding indicator had a greater number of embryos (4.1 +/- 0.5, vs. 1.2 +/- 1.0, vs. 1.8 +/- 1.0 embryos), and a greater percentage of these embryos were viable (43.1 +/- 0.05, vs. 35.5 +/- 0.1, vs. 34.3 +/- 0.1%). Similarly, heifers that showed a greater intensity of activity (as measured by the AAM) had a greater number of embryos collected (10.2 +/- 1.2 vs. 6.0 +/- 1.3 embryos), and a greater percentage of those embryos were viable (53.1 +/- 5.0 vs. 23.4 +/- 5.1%). Longer-duration estrus episodes were associated with a higher percentage of viable embryos (51.2 +/- 5.2 vs. 25.3 +/- 5.3%). In conclusion, stronger estrous intensity was associated with a greater number of total embryos collected and a greater percentage of viable embryos. These results suggest that monitoring the intensity of estrus could be used to predict superovulatory response as well as embryo quality in Holstein heifers. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:00:23Z 2020-12-10T20:00:23Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3168/jds.2019-17772 Journal Of Dairy Science. New York: Elsevier Science Inc, v. 103, n. 6, p. 5641-5646, 2020. 0022-0302 http://hdl.handle.net/11449/196918 10.3168/jds.2019-17772 WOS:000535919900032 |
url |
http://dx.doi.org/10.3168/jds.2019-17772 http://hdl.handle.net/11449/196918 |
identifier_str_mv |
Journal Of Dairy Science. New York: Elsevier Science Inc, v. 103, n. 6, p. 5641-5646, 2020. 0022-0302 10.3168/jds.2019-17772 WOS:000535919900032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Dairy Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5641-5646 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021309705355264 |