Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jenvman.2020.110591 http://hdl.handle.net/11449/195359 |
Resumo: | A three-stage bioethanol bioprocess was developed. Firstly, amylases were obtained from Rhizopus microsporus var. oligosporus using wheat bran in solid-state fermentation. Secondly, amylases hydrolyzed a rice byproduct to make a glucose-rich solution, and this sugar was finally metabolized by Saccharomyces cerevisiae to produce bioethanol. Besides, the secreted enzymes were also partially purified and characterized. The amylase activity (AA) in the crude extract was 358 U/g substrate, and the partially purified enzyme showed the best activity in the 4.0-5.5 pH range. A wide pH stability range (3.5-8.5) was confirmed. The amylase was thermostable up to 60 degrees C. The ion Mn+2 (10 mM) improved by 60% the AA. There was a 54.9% yield in the conversion of rice residues into reducing sugars in 10 h. The glucose-rich solution was undergone fermentation by S. cerevisiae and showed high ethanol efficiency, 95.8% of the theoretical value. These results suggested a promising technology for bioethanol production. |
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Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterizationAmylaseSolid-state fermentationRhizopus microsporus var. oligosporusGlucose syrupBioethanolA three-stage bioethanol bioprocess was developed. Firstly, amylases were obtained from Rhizopus microsporus var. oligosporus using wheat bran in solid-state fermentation. Secondly, amylases hydrolyzed a rice byproduct to make a glucose-rich solution, and this sugar was finally metabolized by Saccharomyces cerevisiae to produce bioethanol. Besides, the secreted enzymes were also partially purified and characterized. The amylase activity (AA) in the crude extract was 358 U/g substrate, and the partially purified enzyme showed the best activity in the 4.0-5.5 pH range. A wide pH stability range (3.5-8.5) was confirmed. The amylase was thermostable up to 60 degrees C. The ion Mn+2 (10 mM) improved by 60% the AA. There was a 54.9% yield in the conversion of rice residues into reducing sugars in 10 h. The glucose-rich solution was undergone fermentation by S. cerevisiae and showed high ethanol efficiency, 95.8% of the theoretical value. These results suggested a promising technology for bioethanol production.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Inst Pesquisa Bioenergia IPBEN, Dept Biotecnol, Lab Biotecnol Ind, Campus Assis,Ave Dom Antonio 2100, Assis 19806900, SP, BrazilUniv Estadual Piaui, Campus Herois Jenipapo, Campo Major, PI, BrazilUniv Sao Paulo, Escola Artes Ciencias & Humanidades EACH, Rua Arlindo Bettio 1000, Sao Paulo 03828000, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeira Preto, Lab Tecnol Enzimat, Ave Cafe S-N, Ribeirao Preto 14040903, SP, BrazilUniv Estadual Paulista, Inst Pesquisa Bioenergia IPBEN, Dept Biotecnol, Lab Biotecnol Ind, Campus Assis,Ave Dom Antonio 2100, Assis 19806900, SP, BrazilCNPq: 134523/2013-0FAPESP: 2014/24188-1Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Estadual PiauiUniversidade de São Paulo (USP)Barros Ranke, Fabiane Fernanda de [UNESP]Shinya, Thais YumiFigueiredo, Franciane Cristina de [UNESP]Fernandez Nunez, Eutimio GustavoCabral, HamiltonNeto, Pedro de Oliva [UNESP]2020-12-10T17:31:50Z2020-12-10T17:31:50Z2020-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.jenvman.2020.110591Journal Of Environmental Management. London: Academic Press Ltd- Elsevier Science Ltd, v. 266, 10 p., 2020.0301-4797http://hdl.handle.net/11449/19535910.1016/j.jenvman.2020.110591WOS:000531083400014Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Environmental Managementinfo:eu-repo/semantics/openAccess2021-10-23T08:05:25Zoai:repositorio.unesp.br:11449/195359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:44.953718Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
title |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
spellingShingle |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization Barros Ranke, Fabiane Fernanda de [UNESP] Amylase Solid-state fermentation Rhizopus microsporus var. oligosporus Glucose syrup Bioethanol |
title_short |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
title_full |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
title_fullStr |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
title_full_unstemmed |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
title_sort |
Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization |
author |
Barros Ranke, Fabiane Fernanda de [UNESP] |
author_facet |
Barros Ranke, Fabiane Fernanda de [UNESP] Shinya, Thais Yumi Figueiredo, Franciane Cristina de [UNESP] Fernandez Nunez, Eutimio Gustavo Cabral, Hamilton Neto, Pedro de Oliva [UNESP] |
author_role |
author |
author2 |
Shinya, Thais Yumi Figueiredo, Franciane Cristina de [UNESP] Fernandez Nunez, Eutimio Gustavo Cabral, Hamilton Neto, Pedro de Oliva [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Estadual Piaui Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Barros Ranke, Fabiane Fernanda de [UNESP] Shinya, Thais Yumi Figueiredo, Franciane Cristina de [UNESP] Fernandez Nunez, Eutimio Gustavo Cabral, Hamilton Neto, Pedro de Oliva [UNESP] |
dc.subject.por.fl_str_mv |
Amylase Solid-state fermentation Rhizopus microsporus var. oligosporus Glucose syrup Bioethanol |
topic |
Amylase Solid-state fermentation Rhizopus microsporus var. oligosporus Glucose syrup Bioethanol |
description |
A three-stage bioethanol bioprocess was developed. Firstly, amylases were obtained from Rhizopus microsporus var. oligosporus using wheat bran in solid-state fermentation. Secondly, amylases hydrolyzed a rice byproduct to make a glucose-rich solution, and this sugar was finally metabolized by Saccharomyces cerevisiae to produce bioethanol. Besides, the secreted enzymes were also partially purified and characterized. The amylase activity (AA) in the crude extract was 358 U/g substrate, and the partially purified enzyme showed the best activity in the 4.0-5.5 pH range. A wide pH stability range (3.5-8.5) was confirmed. The amylase was thermostable up to 60 degrees C. The ion Mn+2 (10 mM) improved by 60% the AA. There was a 54.9% yield in the conversion of rice residues into reducing sugars in 10 h. The glucose-rich solution was undergone fermentation by S. cerevisiae and showed high ethanol efficiency, 95.8% of the theoretical value. These results suggested a promising technology for bioethanol production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:31:50Z 2020-12-10T17:31:50Z 2020-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jenvman.2020.110591 Journal Of Environmental Management. London: Academic Press Ltd- Elsevier Science Ltd, v. 266, 10 p., 2020. 0301-4797 http://hdl.handle.net/11449/195359 10.1016/j.jenvman.2020.110591 WOS:000531083400014 |
url |
http://dx.doi.org/10.1016/j.jenvman.2020.110591 http://hdl.handle.net/11449/195359 |
identifier_str_mv |
Journal Of Environmental Management. London: Academic Press Ltd- Elsevier Science Ltd, v. 266, 10 p., 2020. 0301-4797 10.1016/j.jenvman.2020.110591 WOS:000531083400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Environmental Management |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129574166331392 |