Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

Detalhes bibliográficos
Autor(a) principal: Goulart, A. J. [UNESP]
Data de Publicação: 2005
Outros Autores: Carmona, E. C. [UNESP], Monti, Rubens [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-89132005000300001
http://hdl.handle.net/11449/20054
Resumo: Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.
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spelling Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentationalkaline xylanaseRhizopus stolonifersolid-state fermentationRhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.Univ Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, BrazilUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, BrazilInst Tecnologia ParanaUniversidade Estadual Paulista (Unesp)Goulart, A. J. [UNESP]Carmona, E. C. [UNESP]Monti, Rubens [UNESP]2014-05-20T13:56:04Z2014-05-20T13:56:04Z2005-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article327-333application/pdfhttp://dx.doi.org/10.1590/S1516-89132005000300001Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.1516-8913http://hdl.handle.net/11449/2005410.1590/S1516-89132005000300001S1516-89132005000300001WOS:0002296131000012-s2.0-27944503315WOS000229613100001.pdf4110421764783871Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technology0.6760,281info:eu-repo/semantics/openAccess2024-06-21T12:47:01Zoai:repositorio.unesp.br:11449/20054Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:16:10.721543Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
title Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
spellingShingle Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
Goulart, A. J. [UNESP]
alkaline xylanase
Rhizopus stolonifer
solid-state fermentation
title_short Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
title_full Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
title_fullStr Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
title_full_unstemmed Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
title_sort Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
author Goulart, A. J. [UNESP]
author_facet Goulart, A. J. [UNESP]
Carmona, E. C. [UNESP]
Monti, Rubens [UNESP]
author_role author
author2 Carmona, E. C. [UNESP]
Monti, Rubens [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Goulart, A. J. [UNESP]
Carmona, E. C. [UNESP]
Monti, Rubens [UNESP]
dc.subject.por.fl_str_mv alkaline xylanase
Rhizopus stolonifer
solid-state fermentation
topic alkaline xylanase
Rhizopus stolonifer
solid-state fermentation
description Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
2014-05-20T13:56:04Z
2014-05-20T13:56:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-89132005000300001
Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.
1516-8913
http://hdl.handle.net/11449/20054
10.1590/S1516-89132005000300001
S1516-89132005000300001
WOS:000229613100001
2-s2.0-27944503315
WOS000229613100001.pdf
4110421764783871
url http://dx.doi.org/10.1590/S1516-89132005000300001
http://hdl.handle.net/11449/20054
identifier_str_mv Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.
1516-8913
10.1590/S1516-89132005000300001
S1516-89132005000300001
WOS:000229613100001
2-s2.0-27944503315
WOS000229613100001.pdf
4110421764783871
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Archives of Biology and Technology
0.676
0,281
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 327-333
application/pdf
dc.publisher.none.fl_str_mv Inst Tecnologia Parana
publisher.none.fl_str_mv Inst Tecnologia Parana
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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