Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Caroline Fabri Bittencourt [UNESP]
Data de Publicação: 2015
Outros Autores: Gaeta, Henrique Hessel [UNESP], Belchor, Mariana Novo [UNESP], Ferreira, Marcelo José Pena, Pinho, Marcus Vinícius Terashima [UNESP], Toyama, Daniela de Oliveira, Toyama, Marcos Hikari [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/md13074505
http://hdl.handle.net/11449/131310
Resumo: The aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.) leaves on human thrombin activity. Among the extracts and phases tested, only the ethyl acetate and butanolic partitions significantly inhibited human thrombin activity and the coagulation of plasma in the presence of this enzyme. Chromatographic analyses of the thrombin samples incubated with these phases revealed that different compounds were able to interact with thrombin. The butanolic phase of the MeOH extract had the most potent inhibitory effects, reducing enzymatic activity and thrombin-induced plasma coagulation. Two glycosylated flavonoids in this partition were identified as the most potent inhibitors of human thrombin activity, namely quercetin-3-O-arabinoside (QAra) and quercetin-3-O-rhamnoside (Qn). Chromatographic analyses of thrombin samples incubated with these flavonoids demonstrated the chemical modification of this enzyme, suggesting that the MeOH extract contained other compounds that both induced structural changes in thrombin and diminished its activity. In this article, we show that despite the near absence of the medical use of mangrove compounds, this plant contains natural compounds with potential therapeutic applications.
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spelling Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)Laguncularia racemosaFlavonoidsPlasma coagulationThrombinThe aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.) leaves on human thrombin activity. Among the extracts and phases tested, only the ethyl acetate and butanolic partitions significantly inhibited human thrombin activity and the coagulation of plasma in the presence of this enzyme. Chromatographic analyses of the thrombin samples incubated with these phases revealed that different compounds were able to interact with thrombin. The butanolic phase of the MeOH extract had the most potent inhibitory effects, reducing enzymatic activity and thrombin-induced plasma coagulation. Two glycosylated flavonoids in this partition were identified as the most potent inhibitors of human thrombin activity, namely quercetin-3-O-arabinoside (QAra) and quercetin-3-O-rhamnoside (Qn). Chromatographic analyses of thrombin samples incubated with these flavonoids demonstrated the chemical modification of this enzyme, suggesting that the MeOH extract contained other compounds that both induced structural changes in thrombin and diminished its activity. In this article, we show that despite the near absence of the medical use of mangrove compounds, this plant contains natural compounds with potential therapeutic applications.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, 05508-090 São Paulo, SP, BrazilUnicastelo—Universidade Camilo Castelo Branco, Campus São Paulo, Rua Carolina Fonseca, 584 Itaquera São Paulo-SP, 08230-030 São Paulo, SP, Brazil.Universidade Estadual Paulista Julio de Mesquita Filho, UNESP, Praça Infante Dom Henrique, s/n, 11330-900 São Vicente, SP, BrazilFAPESP: 2013/10938-6Marine DrugsUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Camilo Castelo Branco (UNICASTELO)Rodrigues, Caroline Fabri Bittencourt [UNESP]Gaeta, Henrique Hessel [UNESP]Belchor, Mariana Novo [UNESP]Ferreira, Marcelo José PenaPinho, Marcus Vinícius Terashima [UNESP]Toyama, Daniela de OliveiraToyama, Marcos Hikari [UNESP]2015-12-07T15:33:44Z2015-12-07T15:33:44Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4505-4519application/pdfhttp://dx.doi.org/10.3390/md13074505Marine Drugs, v. 13, n. 7, p. 4505-4519, 2015.1660-3397http://hdl.handle.net/11449/13131010.3390/md13074505PM26287213.pdf857319532754206126287213PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMarine Drugs4.3790,978info:eu-repo/semantics/openAccess2024-01-27T07:01:04Zoai:repositorio.unesp.br:11449/131310Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:51.642808Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
title Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
spellingShingle Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
Rodrigues, Caroline Fabri Bittencourt [UNESP]
Laguncularia racemosa
Flavonoids
Plasma coagulation
Thrombin
title_short Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
title_full Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
title_fullStr Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
title_full_unstemmed Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
title_sort Evaluation of potential thrombin inhibitors from the white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)
author Rodrigues, Caroline Fabri Bittencourt [UNESP]
author_facet Rodrigues, Caroline Fabri Bittencourt [UNESP]
Gaeta, Henrique Hessel [UNESP]
Belchor, Mariana Novo [UNESP]
Ferreira, Marcelo José Pena
Pinho, Marcus Vinícius Terashima [UNESP]
Toyama, Daniela de Oliveira
Toyama, Marcos Hikari [UNESP]
author_role author
author2 Gaeta, Henrique Hessel [UNESP]
Belchor, Mariana Novo [UNESP]
Ferreira, Marcelo José Pena
Pinho, Marcus Vinícius Terashima [UNESP]
Toyama, Daniela de Oliveira
Toyama, Marcos Hikari [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Universidade Camilo Castelo Branco (UNICASTELO)
dc.contributor.author.fl_str_mv Rodrigues, Caroline Fabri Bittencourt [UNESP]
Gaeta, Henrique Hessel [UNESP]
Belchor, Mariana Novo [UNESP]
Ferreira, Marcelo José Pena
Pinho, Marcus Vinícius Terashima [UNESP]
Toyama, Daniela de Oliveira
Toyama, Marcos Hikari [UNESP]
dc.subject.por.fl_str_mv Laguncularia racemosa
Flavonoids
Plasma coagulation
Thrombin
topic Laguncularia racemosa
Flavonoids
Plasma coagulation
Thrombin
description The aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.) leaves on human thrombin activity. Among the extracts and phases tested, only the ethyl acetate and butanolic partitions significantly inhibited human thrombin activity and the coagulation of plasma in the presence of this enzyme. Chromatographic analyses of the thrombin samples incubated with these phases revealed that different compounds were able to interact with thrombin. The butanolic phase of the MeOH extract had the most potent inhibitory effects, reducing enzymatic activity and thrombin-induced plasma coagulation. Two glycosylated flavonoids in this partition were identified as the most potent inhibitors of human thrombin activity, namely quercetin-3-O-arabinoside (QAra) and quercetin-3-O-rhamnoside (Qn). Chromatographic analyses of thrombin samples incubated with these flavonoids demonstrated the chemical modification of this enzyme, suggesting that the MeOH extract contained other compounds that both induced structural changes in thrombin and diminished its activity. In this article, we show that despite the near absence of the medical use of mangrove compounds, this plant contains natural compounds with potential therapeutic applications.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-07T15:33:44Z
2015-12-07T15:33:44Z
2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/md13074505
Marine Drugs, v. 13, n. 7, p. 4505-4519, 2015.
1660-3397
http://hdl.handle.net/11449/131310
10.3390/md13074505
PM26287213.pdf
8573195327542061
26287213
url http://dx.doi.org/10.3390/md13074505
http://hdl.handle.net/11449/131310
identifier_str_mv Marine Drugs, v. 13, n. 7, p. 4505-4519, 2015.
1660-3397
10.3390/md13074505
PM26287213.pdf
8573195327542061
26287213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marine Drugs
4.379
0,978
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dc.format.none.fl_str_mv 4505-4519
application/pdf
dc.publisher.none.fl_str_mv Marine Drugs
publisher.none.fl_str_mv Marine Drugs
dc.source.none.fl_str_mv PubMed
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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