Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization

Detalhes bibliográficos
Autor(a) principal: Santezi, Carolina [UNESP]
Data de Publicação: 2016
Outros Autores: Tanomaru, Juliane M.G. [UNESP], Bagnato, Vanderlei S., Júnior, Osmir B. Oliveira [UNESP], Dovigo, Lívia N. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2016.04.006
http://hdl.handle.net/11449/168697
Resumo: Objective: The present study assessed the susceptibility of salivary pathogens to photodynamic inactivation (PDI), mediated by a water-soluble mixture of curcuminoids (CRM) and LED light. Methods: A 10 mL sample of unstimulated saliva was collected from volunteers. The inoculum was prepared using 9 mL of saline and 1 mL of saliva. Inoculum suspensions were divided into 14 groups and treated according to the description below. Groups that received the PDI treatment (light for 1 min or 5 min and 1.5 g/L or 3.0 g/L of CRM concentration) were called C1.5L1.8, C1.5L9.0, C3.0L1.8, C3.0L9.0. To evaluate the CRM decontamination alone, the C1.5/1,C1.5/5,C3.0/1 and C3.0/5 groups were assessed. Likewise, light alone was evaluated through the L1.8 and L9.0 groups. Chlorhexidine at 0.12% (CLX) for 1 or 5 min was used for the positive control groups (CLX1 and CLX5, respectively); saline was used for 1 or 5 min (CTR1, CTR5, respectively) for the negative control groups. After the tests, serial dilutions were performed, and the resulting samples were plated on blood agar in microaerophilic conditions. The number of colony forming units (CFU/mL) was determined and log10-transformed. Data were analyzed using a One-way Analysis of Variance with Welch correction, followed by the Games Howell's test (α = 0.05). Log reduction (LR) measure for antimicrobial efficacy was also calculated using data from the CTR5 as untreated samples. Results: The CHX5 showed the best antimicrobial result, followed by the CLX1. The antimicrobial effect of CRM was more pronounced when associated with light (PDI), but significantly lower than the CLX5 effect. The C3.0L9.0 protocol showed similar results to the CLX1. Conclusion: The results show that PDI with CRM at the studied concentrations is as effective for oral decontamination in clinical dental care conditions as the CLX at 0.12% for 1 min.
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spelling Potential of curcumin-mediated photodynamic inactivation to reduce oral colonizationBiological contaminationCurcuminPhotochemotherapyObjective: The present study assessed the susceptibility of salivary pathogens to photodynamic inactivation (PDI), mediated by a water-soluble mixture of curcuminoids (CRM) and LED light. Methods: A 10 mL sample of unstimulated saliva was collected from volunteers. The inoculum was prepared using 9 mL of saline and 1 mL of saliva. Inoculum suspensions were divided into 14 groups and treated according to the description below. Groups that received the PDI treatment (light for 1 min or 5 min and 1.5 g/L or 3.0 g/L of CRM concentration) were called C1.5L1.8, C1.5L9.0, C3.0L1.8, C3.0L9.0. To evaluate the CRM decontamination alone, the C1.5/1,C1.5/5,C3.0/1 and C3.0/5 groups were assessed. Likewise, light alone was evaluated through the L1.8 and L9.0 groups. Chlorhexidine at 0.12% (CLX) for 1 or 5 min was used for the positive control groups (CLX1 and CLX5, respectively); saline was used for 1 or 5 min (CTR1, CTR5, respectively) for the negative control groups. After the tests, serial dilutions were performed, and the resulting samples were plated on blood agar in microaerophilic conditions. The number of colony forming units (CFU/mL) was determined and log10-transformed. Data were analyzed using a One-way Analysis of Variance with Welch correction, followed by the Games Howell's test (α = 0.05). Log reduction (LR) measure for antimicrobial efficacy was also calculated using data from the CTR5 as untreated samples. Results: The CHX5 showed the best antimicrobial result, followed by the CLX1. The antimicrobial effect of CRM was more pronounced when associated with light (PDI), but significantly lower than the CLX5 effect. The C3.0L9.0 protocol showed similar results to the CLX1. Conclusion: The results show that PDI with CRM at the studied concentrations is as effective for oral decontamination in clinical dental care conditions as the CLX at 0.12% for 1 min.Araraquara Dental School UNESP- Univ Estadual PaulistaPhysics Institute of São Carlos University of São Paulo (USP)Araraquara Dental School UNESP- Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Santezi, Carolina [UNESP]Tanomaru, Juliane M.G. [UNESP]Bagnato, Vanderlei S.Júnior, Osmir B. Oliveira [UNESP]Dovigo, Lívia N. [UNESP]2018-12-11T16:42:36Z2018-12-11T16:42:36Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article46-52application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2016.04.006Photodiagnosis and Photodynamic Therapy, v. 15, p. 46-52.1873-15971572-1000http://hdl.handle.net/11449/16869710.1016/j.pdpdt.2016.04.0062-s2.0-849712511622-s2.0-84971251162.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2023-10-12T06:09:43Zoai:repositorio.unesp.br:11449/168697Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:36.848113Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
title Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
spellingShingle Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
Santezi, Carolina [UNESP]
Biological contamination
Curcumin
Photochemotherapy
title_short Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
title_full Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
title_fullStr Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
title_full_unstemmed Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
title_sort Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
author Santezi, Carolina [UNESP]
author_facet Santezi, Carolina [UNESP]
Tanomaru, Juliane M.G. [UNESP]
Bagnato, Vanderlei S.
Júnior, Osmir B. Oliveira [UNESP]
Dovigo, Lívia N. [UNESP]
author_role author
author2 Tanomaru, Juliane M.G. [UNESP]
Bagnato, Vanderlei S.
Júnior, Osmir B. Oliveira [UNESP]
Dovigo, Lívia N. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Santezi, Carolina [UNESP]
Tanomaru, Juliane M.G. [UNESP]
Bagnato, Vanderlei S.
Júnior, Osmir B. Oliveira [UNESP]
Dovigo, Lívia N. [UNESP]
dc.subject.por.fl_str_mv Biological contamination
Curcumin
Photochemotherapy
topic Biological contamination
Curcumin
Photochemotherapy
description Objective: The present study assessed the susceptibility of salivary pathogens to photodynamic inactivation (PDI), mediated by a water-soluble mixture of curcuminoids (CRM) and LED light. Methods: A 10 mL sample of unstimulated saliva was collected from volunteers. The inoculum was prepared using 9 mL of saline and 1 mL of saliva. Inoculum suspensions were divided into 14 groups and treated according to the description below. Groups that received the PDI treatment (light for 1 min or 5 min and 1.5 g/L or 3.0 g/L of CRM concentration) were called C1.5L1.8, C1.5L9.0, C3.0L1.8, C3.0L9.0. To evaluate the CRM decontamination alone, the C1.5/1,C1.5/5,C3.0/1 and C3.0/5 groups were assessed. Likewise, light alone was evaluated through the L1.8 and L9.0 groups. Chlorhexidine at 0.12% (CLX) for 1 or 5 min was used for the positive control groups (CLX1 and CLX5, respectively); saline was used for 1 or 5 min (CTR1, CTR5, respectively) for the negative control groups. After the tests, serial dilutions were performed, and the resulting samples were plated on blood agar in microaerophilic conditions. The number of colony forming units (CFU/mL) was determined and log10-transformed. Data were analyzed using a One-way Analysis of Variance with Welch correction, followed by the Games Howell's test (α = 0.05). Log reduction (LR) measure for antimicrobial efficacy was also calculated using data from the CTR5 as untreated samples. Results: The CHX5 showed the best antimicrobial result, followed by the CLX1. The antimicrobial effect of CRM was more pronounced when associated with light (PDI), but significantly lower than the CLX5 effect. The C3.0L9.0 protocol showed similar results to the CLX1. Conclusion: The results show that PDI with CRM at the studied concentrations is as effective for oral decontamination in clinical dental care conditions as the CLX at 0.12% for 1 min.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T16:42:36Z
2018-12-11T16:42:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2016.04.006
Photodiagnosis and Photodynamic Therapy, v. 15, p. 46-52.
1873-1597
1572-1000
http://hdl.handle.net/11449/168697
10.1016/j.pdpdt.2016.04.006
2-s2.0-84971251162
2-s2.0-84971251162.pdf
url http://dx.doi.org/10.1016/j.pdpdt.2016.04.006
http://hdl.handle.net/11449/168697
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 15, p. 46-52.
1873-1597
1572-1000
10.1016/j.pdpdt.2016.04.006
2-s2.0-84971251162
2-s2.0-84971251162.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
0,647
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 46-52
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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