Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fcell.2021.623852 http://hdl.handle.net/11449/210100 |
Resumo: | In several species, oocyte and embryo competence are improved by the addition of endoplasmic reticulum (ER) stress inhibitors to in vitro maturation (IVM) medium and/or in vitro culture (IVC) medium. This study aimed to evaluate the effects of three concentrations of tauroursodeoxycholic acid (TUDCA; 50, 200, and 1,000 mu M), a chemical chaperone for relieving ER stress, during IVM of bovine cumulus-oocyte complexes (COCs) for 24 h. Treated oocytes were analyzed for nuclear maturation, reactive oxygen species (ROS) production, mitochondrial activity, and abundance of target transcripts. In addition, the number of pronuclei in oocytes was evaluated after 18-20 h of insemination, and the rates of blastocyst and hatched blastocyst formation were evaluated after 7 and 8/9 days of culture, respectively. We further evaluated the transcript abundance of embryonic quality markers. Our findings showed that supplementation of IVM medium with 200 mu M of TUDCA decreased ROS production and increased abundance of transcripts related to antioxidant activity in oocytes (CAT, GPX1, and HMOX1) and embryos (GPX1 and PRDX3). Interestingly, high concentration of TUDCA (1,000 mu M) was toxic to oocytes, reducing the nuclear maturation rate, decreasing mitochondrial activity, and increasing the abundance of ER stress (HSPA5) and cellular apoptosis (CASP3 and CD40) related transcripts. The results of this study suggest that treatment with 200 mu M of TUDCA is associated with a greater resistance to oxidative stress and indirectly with ER stress relief in bovine oocytes. |
id |
UNSP_b73d572226f62d80e12a8993edc25156 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/210100 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathwayoocytein vitro maturationTUDCAendoplasmic reticulum stressoxidative stresscattleIn several species, oocyte and embryo competence are improved by the addition of endoplasmic reticulum (ER) stress inhibitors to in vitro maturation (IVM) medium and/or in vitro culture (IVC) medium. This study aimed to evaluate the effects of three concentrations of tauroursodeoxycholic acid (TUDCA; 50, 200, and 1,000 mu M), a chemical chaperone for relieving ER stress, during IVM of bovine cumulus-oocyte complexes (COCs) for 24 h. Treated oocytes were analyzed for nuclear maturation, reactive oxygen species (ROS) production, mitochondrial activity, and abundance of target transcripts. In addition, the number of pronuclei in oocytes was evaluated after 18-20 h of insemination, and the rates of blastocyst and hatched blastocyst formation were evaluated after 7 and 8/9 days of culture, respectively. We further evaluated the transcript abundance of embryonic quality markers. Our findings showed that supplementation of IVM medium with 200 mu M of TUDCA decreased ROS production and increased abundance of transcripts related to antioxidant activity in oocytes (CAT, GPX1, and HMOX1) and embryos (GPX1 and PRDX3). Interestingly, high concentration of TUDCA (1,000 mu M) was toxic to oocytes, reducing the nuclear maturation rate, decreasing mitochondrial activity, and increasing the abundance of ER stress (HSPA5) and cellular apoptosis (CASP3 and CD40) related transcripts. The results of this study suggest that treatment with 200 mu M of TUDCA is associated with a greater resistance to oxidative stress and indirectly with ER stress relief in bovine oocytes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Multiuser Lab Phytomed Pharmacol & Biotechnol Phi, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilUniv Fed Sao Paulo, Dept Biol Sci, Diadema, BrazilSao Paulo State Univ, Sch Vet Med, Dept Prod & Anim Hlth, Aracatuba, BrazilSao Paulo State Univ, Dept Biol Sci, Lab Embryon Micromanipulat, Sch Sci & Languages, Assis, BrazilSao Paulo State Univ, Multiuser Lab Phytomed Pharmacol & Biotechnol Phi, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Sch Vet Med, Dept Prod & Anim Hlth, Aracatuba, BrazilSao Paulo State Univ, Dept Biol Sci, Lab Embryon Micromanipulat, Sch Sci & Languages, Assis, BrazilFAPESP: 2012/50533-2CAPES: 001Frontiers Media SaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Pioltine, Elisa Mariano [UNESP]Costa, Camila Bortoliero [UNESP]Barbosa Latorraca, LaisFranchi, Fernanda Fagali [UNESP]Santos, Priscila Helena dos [UNESP]Mingoti, Gisele Zoccal [UNESP]Paula-Lopes, Fabiola Freitas deNogueira, Marcelo Fabio Gouveia [UNESP]2021-06-25T12:39:45Z2021-06-25T12:39:45Z2021-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.3389/fcell.2021.623852Frontiers In Cell And Developmental Biology. Lausanne: Frontiers Media Sa, v. 9, 12 p., 2021.2296-634Xhttp://hdl.handle.net/11449/21010010.3389/fcell.2021.623852WOS:000625814100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Cell And Developmental Biologyinfo:eu-repo/semantics/openAccess2024-09-04T19:14:52Zoai:repositorio.unesp.br:11449/210100Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:14:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
title |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
spellingShingle |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway Pioltine, Elisa Mariano [UNESP] oocyte in vitro maturation TUDCA endoplasmic reticulum stress oxidative stress cattle |
title_short |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
title_full |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
title_fullStr |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
title_full_unstemmed |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
title_sort |
Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway |
author |
Pioltine, Elisa Mariano [UNESP] |
author_facet |
Pioltine, Elisa Mariano [UNESP] Costa, Camila Bortoliero [UNESP] Barbosa Latorraca, Lais Franchi, Fernanda Fagali [UNESP] Santos, Priscila Helena dos [UNESP] Mingoti, Gisele Zoccal [UNESP] Paula-Lopes, Fabiola Freitas de Nogueira, Marcelo Fabio Gouveia [UNESP] |
author_role |
author |
author2 |
Costa, Camila Bortoliero [UNESP] Barbosa Latorraca, Lais Franchi, Fernanda Fagali [UNESP] Santos, Priscila Helena dos [UNESP] Mingoti, Gisele Zoccal [UNESP] Paula-Lopes, Fabiola Freitas de Nogueira, Marcelo Fabio Gouveia [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Pioltine, Elisa Mariano [UNESP] Costa, Camila Bortoliero [UNESP] Barbosa Latorraca, Lais Franchi, Fernanda Fagali [UNESP] Santos, Priscila Helena dos [UNESP] Mingoti, Gisele Zoccal [UNESP] Paula-Lopes, Fabiola Freitas de Nogueira, Marcelo Fabio Gouveia [UNESP] |
dc.subject.por.fl_str_mv |
oocyte in vitro maturation TUDCA endoplasmic reticulum stress oxidative stress cattle |
topic |
oocyte in vitro maturation TUDCA endoplasmic reticulum stress oxidative stress cattle |
description |
In several species, oocyte and embryo competence are improved by the addition of endoplasmic reticulum (ER) stress inhibitors to in vitro maturation (IVM) medium and/or in vitro culture (IVC) medium. This study aimed to evaluate the effects of three concentrations of tauroursodeoxycholic acid (TUDCA; 50, 200, and 1,000 mu M), a chemical chaperone for relieving ER stress, during IVM of bovine cumulus-oocyte complexes (COCs) for 24 h. Treated oocytes were analyzed for nuclear maturation, reactive oxygen species (ROS) production, mitochondrial activity, and abundance of target transcripts. In addition, the number of pronuclei in oocytes was evaluated after 18-20 h of insemination, and the rates of blastocyst and hatched blastocyst formation were evaluated after 7 and 8/9 days of culture, respectively. We further evaluated the transcript abundance of embryonic quality markers. Our findings showed that supplementation of IVM medium with 200 mu M of TUDCA decreased ROS production and increased abundance of transcripts related to antioxidant activity in oocytes (CAT, GPX1, and HMOX1) and embryos (GPX1 and PRDX3). Interestingly, high concentration of TUDCA (1,000 mu M) was toxic to oocytes, reducing the nuclear maturation rate, decreasing mitochondrial activity, and increasing the abundance of ER stress (HSPA5) and cellular apoptosis (CASP3 and CD40) related transcripts. The results of this study suggest that treatment with 200 mu M of TUDCA is associated with a greater resistance to oxidative stress and indirectly with ER stress relief in bovine oocytes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:39:45Z 2021-06-25T12:39:45Z 2021-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fcell.2021.623852 Frontiers In Cell And Developmental Biology. Lausanne: Frontiers Media Sa, v. 9, 12 p., 2021. 2296-634X http://hdl.handle.net/11449/210100 10.3389/fcell.2021.623852 WOS:000625814100001 |
url |
http://dx.doi.org/10.3389/fcell.2021.623852 http://hdl.handle.net/11449/210100 |
identifier_str_mv |
Frontiers In Cell And Developmental Biology. Lausanne: Frontiers Media Sa, v. 9, 12 p., 2021. 2296-634X 10.3389/fcell.2021.623852 WOS:000625814100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Cell And Developmental Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021359219113984 |