Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jclepro.2019.06.211 http://hdl.handle.net/11449/190431 |
Resumo: | In recent years, forest breeding programs have increased Eucalyptus production for commercial purposes; however, high-performing Eucalyptus clones have had problems with propagation, especially when rooting. Nevertheless, studies have shown that inoculation of microorganisms producing indole-3-acetic acid (IAA) is an especially productive procedure to help these clones breed. In this study, we evaluated the production of IAA and analogues in 16 microbial strains. For the first time, a high IAA production was described in the strain Aspergillus flavipes (ATCC® 16814™), and A. flavipes was shown by LC-MS/MS to produce IAA through a tryptophan-dependent biosynthetic pathway. A. flavipes reached the highest IAA production when cultivated under solid-state fermentation in an optimized medium composed of soybean bran, water and tryptophan. We mixed the fermentation products in solid form (SF) and liquid form (LF) with the substrate Carolina I® and then planted the cuttings of the hybrid Eucalyptus grandis x E. urophylla (clone IPB2). In fact, treatments with 40–120 mg kg−1 of SF increased the adventitious rooting rate, root length and both root fresh and dry mass, while 120 mg kg−1 of LF increased root length and dry mass. Additionally, there was no toxicity on fibroblasts (NIH/3t3), and, therefore, the plant biostimulant was confirmed as a novel, non-toxic, and eco-friendly solution. |
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Aspergillus flavipes as a novel biostimulant for rooting-enhancement of EucalyptusCytotoxicityIndole-3-acetic acidLC-MS/MSSolid-state fermentationVegetative propagationIn recent years, forest breeding programs have increased Eucalyptus production for commercial purposes; however, high-performing Eucalyptus clones have had problems with propagation, especially when rooting. Nevertheless, studies have shown that inoculation of microorganisms producing indole-3-acetic acid (IAA) is an especially productive procedure to help these clones breed. In this study, we evaluated the production of IAA and analogues in 16 microbial strains. For the first time, a high IAA production was described in the strain Aspergillus flavipes (ATCC® 16814™), and A. flavipes was shown by LC-MS/MS to produce IAA through a tryptophan-dependent biosynthetic pathway. A. flavipes reached the highest IAA production when cultivated under solid-state fermentation in an optimized medium composed of soybean bran, water and tryptophan. We mixed the fermentation products in solid form (SF) and liquid form (LF) with the substrate Carolina I® and then planted the cuttings of the hybrid Eucalyptus grandis x E. urophylla (clone IPB2). In fact, treatments with 40–120 mg kg−1 of SF increased the adventitious rooting rate, root length and both root fresh and dry mass, while 120 mg kg−1 of LF increased root length and dry mass. Additionally, there was no toxicity on fibroblasts (NIH/3t3), and, therefore, the plant biostimulant was confirmed as a novel, non-toxic, and eco-friendly solution.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação Oswaldo CruzUniversidade Estadual de CampinasSão Paulo State University (UNESP) Institute of BiosciencesSão Paulo State University (UNESP) School of AgricultureTechnische Universität Dresden Institute of BotanySão Paulo State University (UNESP) Institute of BiosciencesSão Paulo State University (UNESP) School of AgricultureCAPES: 001CAPES: 88881.133019/2016–01Universidade Estadual Paulista (Unesp)Institute of BotanyZanoni do Prado, Débora [UNESP]Oliveira, Samara Louzada [UNESP]Okino-Delgado, Clarissa Hamaio [UNESP]Auer, SusannLudwig-Müller, JuttaRibeiro da Silva, Magali [UNESP]Júnior da Costa Fernandes, Célio [UNESP]Carbonari, Caio Antonio [UNESP]Zambuzzi, William Fernando [UNESP]Fleuri, Luciana Francisco [UNESP]2019-10-06T17:13:01Z2019-10-06T17:13:01Z2019-10-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article681-689http://dx.doi.org/10.1016/j.jclepro.2019.06.211Journal of Cleaner Production, v. 234, p. 681-689.0959-6526http://hdl.handle.net/11449/19043110.1016/j.jclepro.2019.06.2112-s2.0-850678591316495148747049688Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cleaner Productioninfo:eu-repo/semantics/openAccess2021-10-22T18:33:47Zoai:repositorio.unesp.br:11449/190431Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:31:11.183120Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
title |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
spellingShingle |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus Zanoni do Prado, Débora [UNESP] Cytotoxicity Indole-3-acetic acid LC-MS/MS Solid-state fermentation Vegetative propagation |
title_short |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
title_full |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
title_fullStr |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
title_full_unstemmed |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
title_sort |
Aspergillus flavipes as a novel biostimulant for rooting-enhancement of Eucalyptus |
author |
Zanoni do Prado, Débora [UNESP] |
author_facet |
Zanoni do Prado, Débora [UNESP] Oliveira, Samara Louzada [UNESP] Okino-Delgado, Clarissa Hamaio [UNESP] Auer, Susann Ludwig-Müller, Jutta Ribeiro da Silva, Magali [UNESP] Júnior da Costa Fernandes, Célio [UNESP] Carbonari, Caio Antonio [UNESP] Zambuzzi, William Fernando [UNESP] Fleuri, Luciana Francisco [UNESP] |
author_role |
author |
author2 |
Oliveira, Samara Louzada [UNESP] Okino-Delgado, Clarissa Hamaio [UNESP] Auer, Susann Ludwig-Müller, Jutta Ribeiro da Silva, Magali [UNESP] Júnior da Costa Fernandes, Célio [UNESP] Carbonari, Caio Antonio [UNESP] Zambuzzi, William Fernando [UNESP] Fleuri, Luciana Francisco [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute of Botany |
dc.contributor.author.fl_str_mv |
Zanoni do Prado, Débora [UNESP] Oliveira, Samara Louzada [UNESP] Okino-Delgado, Clarissa Hamaio [UNESP] Auer, Susann Ludwig-Müller, Jutta Ribeiro da Silva, Magali [UNESP] Júnior da Costa Fernandes, Célio [UNESP] Carbonari, Caio Antonio [UNESP] Zambuzzi, William Fernando [UNESP] Fleuri, Luciana Francisco [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Indole-3-acetic acid LC-MS/MS Solid-state fermentation Vegetative propagation |
topic |
Cytotoxicity Indole-3-acetic acid LC-MS/MS Solid-state fermentation Vegetative propagation |
description |
In recent years, forest breeding programs have increased Eucalyptus production for commercial purposes; however, high-performing Eucalyptus clones have had problems with propagation, especially when rooting. Nevertheless, studies have shown that inoculation of microorganisms producing indole-3-acetic acid (IAA) is an especially productive procedure to help these clones breed. In this study, we evaluated the production of IAA and analogues in 16 microbial strains. For the first time, a high IAA production was described in the strain Aspergillus flavipes (ATCC® 16814™), and A. flavipes was shown by LC-MS/MS to produce IAA through a tryptophan-dependent biosynthetic pathway. A. flavipes reached the highest IAA production when cultivated under solid-state fermentation in an optimized medium composed of soybean bran, water and tryptophan. We mixed the fermentation products in solid form (SF) and liquid form (LF) with the substrate Carolina I® and then planted the cuttings of the hybrid Eucalyptus grandis x E. urophylla (clone IPB2). In fact, treatments with 40–120 mg kg−1 of SF increased the adventitious rooting rate, root length and both root fresh and dry mass, while 120 mg kg−1 of LF increased root length and dry mass. Additionally, there was no toxicity on fibroblasts (NIH/3t3), and, therefore, the plant biostimulant was confirmed as a novel, non-toxic, and eco-friendly solution. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:13:01Z 2019-10-06T17:13:01Z 2019-10-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jclepro.2019.06.211 Journal of Cleaner Production, v. 234, p. 681-689. 0959-6526 http://hdl.handle.net/11449/190431 10.1016/j.jclepro.2019.06.211 2-s2.0-85067859131 6495148747049688 |
url |
http://dx.doi.org/10.1016/j.jclepro.2019.06.211 http://hdl.handle.net/11449/190431 |
identifier_str_mv |
Journal of Cleaner Production, v. 234, p. 681-689. 0959-6526 10.1016/j.jclepro.2019.06.211 2-s2.0-85067859131 6495148747049688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Cleaner Production |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
681-689 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128372858945536 |