TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10853-021-06373-4 http://hdl.handle.net/11449/229263 |
Resumo: | The development of new cell carrier systems is crucial for application in regenerative medicine, once they deliver the cells to the injured tissue to trigger the repair and stimulate the regeneration. Here we report the preparation of a cell carrier system in fiber shape, in which the cells osteo-1 are incorporated. For the fiber formation, the oxidized cellulose nanofibers suspension loaded with cells is complexed with calcium ions in a pulling process giving rise to the fiber loaded with cells, with dimensions which allow it to handle. The microscopic images showed the success of the proposed method to incorporate the cells into the fibers. The results of the in vitro viability tests indicated the capability of the fibers to keep the cells alive and to mineralize them, indicating that their osteogenic capability was not affected. In addition, the fiber disintegration studies showed the system is capable of releasing the cells, suggesting the potential of the fibers as a new assembled hydrogel carrier cell therapy. |
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TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of conceptThe development of new cell carrier systems is crucial for application in regenerative medicine, once they deliver the cells to the injured tissue to trigger the repair and stimulate the regeneration. Here we report the preparation of a cell carrier system in fiber shape, in which the cells osteo-1 are incorporated. For the fiber formation, the oxidized cellulose nanofibers suspension loaded with cells is complexed with calcium ions in a pulling process giving rise to the fiber loaded with cells, with dimensions which allow it to handle. The microscopic images showed the success of the proposed method to incorporate the cells into the fibers. The results of the in vitro viability tests indicated the capability of the fibers to keep the cells alive and to mineralize them, indicating that their osteogenic capability was not affected. In addition, the fiber disintegration studies showed the system is capable of releasing the cells, suggesting the potential of the fibers as a new assembled hydrogel carrier cell therapy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)University of Araraquara, UNIARA, R. Carlos Gomes, 1217Laboratory of Confocal Microscopy Department of Dental Materials and Prosthodontics Faculty of Dentistry São Paulo State University (Unesp)Department of Bioproducts and Biosystems School of Chemical Engineering Aalto UniversittyDepartment of Materials Engineering Sao Carlos School of Engineering University of Sao Paulo, Av. Joao Dagnone 1100Laboratory of Confocal Microscopy Department of Dental Materials and Prosthodontics Faculty of Dentistry São Paulo State University (Unesp)FAPESP: 2017/18782-6FAPESP: 2018/06555-8CNPq: 303847/2019-0CNPq: 307124/2015-0University of AraraquaraUniversidade Estadual Paulista (UNESP)Aalto UniversittyUniversidade de São Paulo (USP)Rios, Mariana AlvesBarbugli, Paula Aboud [UNESP]Iemma, Mônica Rosas CostaGrande, RafaelCarvalho, Antônio José FelixTrovatti, Eliane2022-04-29T08:31:28Z2022-04-29T08:31:28Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16661-16670http://dx.doi.org/10.1007/s10853-021-06373-4Journal of Materials Science, v. 56, n. 29, p. 16661-16670, 2021.1573-48030022-2461http://hdl.handle.net/11449/22926310.1007/s10853-021-06373-42-s2.0-85111795290Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Scienceinfo:eu-repo/semantics/openAccess2024-04-11T19:33:51Zoai:repositorio.unesp.br:11449/229263Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:53:09.215525Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
title |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
spellingShingle |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept Rios, Mariana Alves |
title_short |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
title_full |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
title_fullStr |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
title_full_unstemmed |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
title_sort |
TEMPO-oxidized cellulose poly-ionic drawn fiber, a cell support system proof of concept |
author |
Rios, Mariana Alves |
author_facet |
Rios, Mariana Alves Barbugli, Paula Aboud [UNESP] Iemma, Mônica Rosas Costa Grande, Rafael Carvalho, Antônio José Felix Trovatti, Eliane |
author_role |
author |
author2 |
Barbugli, Paula Aboud [UNESP] Iemma, Mônica Rosas Costa Grande, Rafael Carvalho, Antônio José Felix Trovatti, Eliane |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
University of Araraquara Universidade Estadual Paulista (UNESP) Aalto Universitty Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rios, Mariana Alves Barbugli, Paula Aboud [UNESP] Iemma, Mônica Rosas Costa Grande, Rafael Carvalho, Antônio José Felix Trovatti, Eliane |
description |
The development of new cell carrier systems is crucial for application in regenerative medicine, once they deliver the cells to the injured tissue to trigger the repair and stimulate the regeneration. Here we report the preparation of a cell carrier system in fiber shape, in which the cells osteo-1 are incorporated. For the fiber formation, the oxidized cellulose nanofibers suspension loaded with cells is complexed with calcium ions in a pulling process giving rise to the fiber loaded with cells, with dimensions which allow it to handle. The microscopic images showed the success of the proposed method to incorporate the cells into the fibers. The results of the in vitro viability tests indicated the capability of the fibers to keep the cells alive and to mineralize them, indicating that their osteogenic capability was not affected. In addition, the fiber disintegration studies showed the system is capable of releasing the cells, suggesting the potential of the fibers as a new assembled hydrogel carrier cell therapy. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-29T08:31:28Z 2022-04-29T08:31:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10853-021-06373-4 Journal of Materials Science, v. 56, n. 29, p. 16661-16670, 2021. 1573-4803 0022-2461 http://hdl.handle.net/11449/229263 10.1007/s10853-021-06373-4 2-s2.0-85111795290 |
url |
http://dx.doi.org/10.1007/s10853-021-06373-4 http://hdl.handle.net/11449/229263 |
identifier_str_mv |
Journal of Materials Science, v. 56, n. 29, p. 16661-16670, 2021. 1573-4803 0022-2461 10.1007/s10853-021-06373-4 2-s2.0-85111795290 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Materials Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16661-16670 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128715502125056 |