Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-2577.04119 http://hdl.handle.net/11449/183868 |
Resumo: | AbstractIntroduction The use of bone substitutes in grafting procedures as an alternative of the use of autogenous bone graft has been indicated, however, the direct comparison between these biomaterials has been little explored.Objective To evaluate the effect of different osteoconductive bone substitutes on the bone repair in critical-sized defects (CSDs) in rat calvaria.Material and method One CSD with an 8 mm diameter was made in each of the 40 rats used in this study. The animals were randomly allocated into 5 groups (n=8), according to the type of bone substitute used to fill the CSD: COA (Coagulum); AUT (autogenous bone); DBB (deproteinized bovine bone graft); HA/TCP (biphasic ceramic composed of hydroxyapatite and β-phosphate tricalcium); and TCP (β-phosphate tricalcium). A microtomographic analysis was performed to evaluate the remaining defect linear length (DLL) of the CSD and the volume of the mineralized tissues (MT) within the CSD at 3, 7, 15 and 30 days after the surgical procedure. In addition, a histometric analysis was performed to evaluate the composition of the repaired bone tissue (% Bone and % Biomaterial) at the 30-day period.Result It was shown that the COA had the lowest DLL and MT within the CSD. In addition, the COA presented the highest % of bone in CSD. The DBB had a higher MT and a higher % of bone substitute particles in the CSD than the AUT and TCP groups. The DBB and AUT groups presented higher % of bone in the CSD than the TCP group.Conclusion The use of the DBB promoted a better pattern of bone volume gain and formation compared to TCP and HA / TCP but was biologically inferior to the AUT. |
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Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvariaAvaliação do reparo ósseo induzido por diferentes substitutos ósseos em modelo de defeito crítico de calvária de ratosHistologyx-ray microtomographybone regenerationbone substitutesHistologiamicrotomografiaregeneração ósseasubstitutos ósseosAbstractIntroduction The use of bone substitutes in grafting procedures as an alternative of the use of autogenous bone graft has been indicated, however, the direct comparison between these biomaterials has been little explored.Objective To evaluate the effect of different osteoconductive bone substitutes on the bone repair in critical-sized defects (CSDs) in rat calvaria.Material and method One CSD with an 8 mm diameter was made in each of the 40 rats used in this study. The animals were randomly allocated into 5 groups (n=8), according to the type of bone substitute used to fill the CSD: COA (Coagulum); AUT (autogenous bone); DBB (deproteinized bovine bone graft); HA/TCP (biphasic ceramic composed of hydroxyapatite and β-phosphate tricalcium); and TCP (β-phosphate tricalcium). A microtomographic analysis was performed to evaluate the remaining defect linear length (DLL) of the CSD and the volume of the mineralized tissues (MT) within the CSD at 3, 7, 15 and 30 days after the surgical procedure. In addition, a histometric analysis was performed to evaluate the composition of the repaired bone tissue (% Bone and % Biomaterial) at the 30-day period.Result It was shown that the COA had the lowest DLL and MT within the CSD. In addition, the COA presented the highest % of bone in CSD. The DBB had a higher MT and a higher % of bone substitute particles in the CSD than the AUT and TCP groups. The DBB and AUT groups presented higher % of bone in the CSD than the TCP group.Conclusion The use of the DBB promoted a better pattern of bone volume gain and formation compared to TCP and HA / TCP but was biologically inferior to the AUT.ResumoIntrodução A utilização de substitutos ósseos em procedimentos de enxertia de forma alternativa ao uso do osso autógeno tem sido indicada, entretanto a comparação direta entre esses biomateriais tem sido pouco explorada.Objetivo Avaliar o efeito de diferentes biomateriais osteocondutores sobre o reparo de defeitos críticos em calvárias (DCC) de ratos.Material e método Foram utilizados 40 ratos que foram submetidos a confecção de um DCC com 8 mm de diâmetro. Os animais foram aleatoriamente divididos em 5 grupos com 8 animais, de acordo com o tipo de biomaterial utilizado para preencher os DCC: Grupo COA (coágulo); Grupo AUT (osso autógeno); Grupo OBD (osso bovino desproteinizado); Grupo HA/ TCP (cerâmica bifásica composta de hidroxiapatita e β fosfato tricálcio); Grupo TCP (β fosfato tricálcio). Foram executadas análise microtomográfica para avaliação do comprimento linear remanescente (DLL) do DCC e o volume dos tecidos mineralizados (MT) dentro do DCC nos períodos de 3, 7, 15 e 30 dias após cirurgia. Adicionalmente, foi executado análise histométrica para avaliar a composição do tecido ósseo reparado (% Osso e % Biomaterial) no período de 30 dias.Resultado O grupo COA apresentou o menor DLL e MT dentro do DCC e maior % osso do que os outros grupos. O grupo OBD apresentou maior volume de tecidos mineralizados e maior % biomaterial do que o grupo os grupos AUT e TCP. Os grupos OBD e AUT apresentaram maior % osso que o grupo TCP.Conclusão O OBD promoveu melhor padrão de aumento de disponibilidade óssea e qualidade do osso reparado em comparação ao TCP e HA/TCP, porém biologicamente inferior ao grupo AUT.Universidade Estadual Paulista Faculdade de Odontologia de Araraquara Departmento de Diagnóstico e CirurgiaUniversidade Federal de Uberlândia Departmento de PeriodontiaUniversidade Estadual Paulista Faculdade de Odontologia de Araraquara Departmento de Diagnóstico e CirurgiaUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia Departmento de PeriodontiaAroni, Mauricio Andrés TinajeroCosta Neto, Paulo Firmino DaOliveira, Guilherme José Pimentel Lopes DeMarcantonio, Rosemary Adriana ChiériciMarcantonio Junior, Elcio2019-10-03T17:32:03Z2019-10-03T17:32:03Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1807-2577.04119Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019.1807-2577http://hdl.handle.net/11449/18386810.1590/1807-2577.04119S1807-25772019000100410S1807-25772019000100410.pdf61008594658719290000-0003-1294-2305SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Odontologia da UNESPinfo:eu-repo/semantics/openAccess2024-01-29T06:32:04Zoai:repositorio.unesp.br:11449/183868Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:14:54.361325Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria Avaliação do reparo ósseo induzido por diferentes substitutos ósseos em modelo de defeito crítico de calvária de ratos |
title |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
spellingShingle |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria Aroni, Mauricio Andrés Tinajero Histology x-ray microtomography bone regeneration bone substitutes Histologia microtomografia regeneração óssea substitutos ósseos |
title_short |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
title_full |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
title_fullStr |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
title_full_unstemmed |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
title_sort |
Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria |
author |
Aroni, Mauricio Andrés Tinajero |
author_facet |
Aroni, Mauricio Andrés Tinajero Costa Neto, Paulo Firmino Da Oliveira, Guilherme José Pimentel Lopes De Marcantonio, Rosemary Adriana Chiérici Marcantonio Junior, Elcio |
author_role |
author |
author2 |
Costa Neto, Paulo Firmino Da Oliveira, Guilherme José Pimentel Lopes De Marcantonio, Rosemary Adriana Chiérici Marcantonio Junior, Elcio |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia Departmento de Periodontia |
dc.contributor.author.fl_str_mv |
Aroni, Mauricio Andrés Tinajero Costa Neto, Paulo Firmino Da Oliveira, Guilherme José Pimentel Lopes De Marcantonio, Rosemary Adriana Chiérici Marcantonio Junior, Elcio |
dc.subject.por.fl_str_mv |
Histology x-ray microtomography bone regeneration bone substitutes Histologia microtomografia regeneração óssea substitutos ósseos |
topic |
Histology x-ray microtomography bone regeneration bone substitutes Histologia microtomografia regeneração óssea substitutos ósseos |
description |
AbstractIntroduction The use of bone substitutes in grafting procedures as an alternative of the use of autogenous bone graft has been indicated, however, the direct comparison between these biomaterials has been little explored.Objective To evaluate the effect of different osteoconductive bone substitutes on the bone repair in critical-sized defects (CSDs) in rat calvaria.Material and method One CSD with an 8 mm diameter was made in each of the 40 rats used in this study. The animals were randomly allocated into 5 groups (n=8), according to the type of bone substitute used to fill the CSD: COA (Coagulum); AUT (autogenous bone); DBB (deproteinized bovine bone graft); HA/TCP (biphasic ceramic composed of hydroxyapatite and β-phosphate tricalcium); and TCP (β-phosphate tricalcium). A microtomographic analysis was performed to evaluate the remaining defect linear length (DLL) of the CSD and the volume of the mineralized tissues (MT) within the CSD at 3, 7, 15 and 30 days after the surgical procedure. In addition, a histometric analysis was performed to evaluate the composition of the repaired bone tissue (% Bone and % Biomaterial) at the 30-day period.Result It was shown that the COA had the lowest DLL and MT within the CSD. In addition, the COA presented the highest % of bone in CSD. The DBB had a higher MT and a higher % of bone substitute particles in the CSD than the AUT and TCP groups. The DBB and AUT groups presented higher % of bone in the CSD than the TCP group.Conclusion The use of the DBB promoted a better pattern of bone volume gain and formation compared to TCP and HA / TCP but was biologically inferior to the AUT. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03T17:32:03Z 2019-10-03T17:32:03Z 2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-2577.04119 Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019. 1807-2577 http://hdl.handle.net/11449/183868 10.1590/1807-2577.04119 S1807-25772019000100410 S1807-25772019000100410.pdf 6100859465871929 0000-0003-1294-2305 |
url |
http://dx.doi.org/10.1590/1807-2577.04119 http://hdl.handle.net/11449/183868 |
identifier_str_mv |
Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019. 1807-2577 10.1590/1807-2577.04119 S1807-25772019000100410 S1807-25772019000100410.pdf 6100859465871929 0000-0003-1294-2305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Odontologia da UNESP |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129599766265856 |