Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12155-020-10187-8 http://hdl.handle.net/11449/202055 |
Resumo: | The sugarcane bagasse is a heterogeneous material and needs a pretreatment to breakdown its complex structure to make cellulose accessible to enzyme action. This study aimed to evaluate pseudo-lignin formation, enzymatic hydrolysis of sugarcane fractions (leaf, external fraction, internode, and node), and bagasse after partial delignification and acid pretreatment. The leaf and external fraction presented the highest content of lignin, and external fraction was the most recalcitrant material resulting in lower glucose release. Pretreatment with diluted sulfuric acid (20% m/m or 2% m/v) generated 5 g/L of acetic acid and 2.07 g/L of 5-hydroxymethylfurfural (external fraction in natura and leaf extractive-free, respectively). Furfural ranged between 0.11 g/L (node delignified) and 0.06 g/L (leaf, external fraction, and node in natura). A decrease was observed in pseudo-lignin formed with extractive-free and delignified biomasses, with different structure compared with non-delignified samples. The biomass partial delignification and subsequent pretreatment with dilute acid generate a material with fragmented anatomical structure, with improved cellulose accessibility, favoring enzymatic hydrolysis achieving more than 90% of glucose yield (up to 12 g/L). This study has shown strategies to decrease material heterogeneity and avoid pseudo-lignin formation as it results in lower recalcitrance and better efficiency of the enzymatic hydrolysis. |
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Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic DigestibilityCelluloseDiluted acid pretreatmentExtractivesGlucoseSugarcane fractionThe sugarcane bagasse is a heterogeneous material and needs a pretreatment to breakdown its complex structure to make cellulose accessible to enzyme action. This study aimed to evaluate pseudo-lignin formation, enzymatic hydrolysis of sugarcane fractions (leaf, external fraction, internode, and node), and bagasse after partial delignification and acid pretreatment. The leaf and external fraction presented the highest content of lignin, and external fraction was the most recalcitrant material resulting in lower glucose release. Pretreatment with diluted sulfuric acid (20% m/m or 2% m/v) generated 5 g/L of acetic acid and 2.07 g/L of 5-hydroxymethylfurfural (external fraction in natura and leaf extractive-free, respectively). Furfural ranged between 0.11 g/L (node delignified) and 0.06 g/L (leaf, external fraction, and node in natura). A decrease was observed in pseudo-lignin formed with extractive-free and delignified biomasses, with different structure compared with non-delignified samples. The biomass partial delignification and subsequent pretreatment with dilute acid generate a material with fragmented anatomical structure, with improved cellulose accessibility, favoring enzymatic hydrolysis achieving more than 90% of glucose yield (up to 12 g/L). This study has shown strategies to decrease material heterogeneity and avoid pseudo-lignin formation as it results in lower recalcitrance and better efficiency of the enzymatic hydrolysis.Institute for Research in Bioenergy (IPBEN) São Paulo State University (UNESP)Laboratory of Microscopy Applied to Life Science(Lamav) National Institute of Metrology Quality and Technology (Inmetro)Institute for Research in Bioenergy (IPBEN) São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Quality and Technology (Inmetro)Schmatz, Alison Andrei [UNESP]Salazar-Bryam, Ana Maria [UNESP]Contiero, Jonas [UNESP]Sant’Anna, CelsoBrienzo, Michel [UNESP]2020-12-12T02:48:42Z2020-12-12T02:48:42Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12155-020-10187-8Bioenergy Research.1939-12421939-1234http://hdl.handle.net/11449/20205510.1007/s12155-020-10187-82-s2.0-850899898808251885707409794Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioenergy Researchinfo:eu-repo/semantics/openAccess2021-10-23T06:52:13Zoai:repositorio.unesp.br:11449/202055Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:58.641001Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
title |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
spellingShingle |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility Schmatz, Alison Andrei [UNESP] Cellulose Diluted acid pretreatment Extractives Glucose Sugarcane fraction |
title_short |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
title_full |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
title_fullStr |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
title_full_unstemmed |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
title_sort |
Pseudo-Lignin Content Decreased with Hemicellulose and Lignin Removal, Improving Cellulose Accessibility, and Enzymatic Digestibility |
author |
Schmatz, Alison Andrei [UNESP] |
author_facet |
Schmatz, Alison Andrei [UNESP] Salazar-Bryam, Ana Maria [UNESP] Contiero, Jonas [UNESP] Sant’Anna, Celso Brienzo, Michel [UNESP] |
author_role |
author |
author2 |
Salazar-Bryam, Ana Maria [UNESP] Contiero, Jonas [UNESP] Sant’Anna, Celso Brienzo, Michel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Quality and Technology (Inmetro) |
dc.contributor.author.fl_str_mv |
Schmatz, Alison Andrei [UNESP] Salazar-Bryam, Ana Maria [UNESP] Contiero, Jonas [UNESP] Sant’Anna, Celso Brienzo, Michel [UNESP] |
dc.subject.por.fl_str_mv |
Cellulose Diluted acid pretreatment Extractives Glucose Sugarcane fraction |
topic |
Cellulose Diluted acid pretreatment Extractives Glucose Sugarcane fraction |
description |
The sugarcane bagasse is a heterogeneous material and needs a pretreatment to breakdown its complex structure to make cellulose accessible to enzyme action. This study aimed to evaluate pseudo-lignin formation, enzymatic hydrolysis of sugarcane fractions (leaf, external fraction, internode, and node), and bagasse after partial delignification and acid pretreatment. The leaf and external fraction presented the highest content of lignin, and external fraction was the most recalcitrant material resulting in lower glucose release. Pretreatment with diluted sulfuric acid (20% m/m or 2% m/v) generated 5 g/L of acetic acid and 2.07 g/L of 5-hydroxymethylfurfural (external fraction in natura and leaf extractive-free, respectively). Furfural ranged between 0.11 g/L (node delignified) and 0.06 g/L (leaf, external fraction, and node in natura). A decrease was observed in pseudo-lignin formed with extractive-free and delignified biomasses, with different structure compared with non-delignified samples. The biomass partial delignification and subsequent pretreatment with dilute acid generate a material with fragmented anatomical structure, with improved cellulose accessibility, favoring enzymatic hydrolysis achieving more than 90% of glucose yield (up to 12 g/L). This study has shown strategies to decrease material heterogeneity and avoid pseudo-lignin formation as it results in lower recalcitrance and better efficiency of the enzymatic hydrolysis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:48:42Z 2020-12-12T02:48:42Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12155-020-10187-8 Bioenergy Research. 1939-1242 1939-1234 http://hdl.handle.net/11449/202055 10.1007/s12155-020-10187-8 2-s2.0-85089989880 8251885707409794 |
url |
http://dx.doi.org/10.1007/s12155-020-10187-8 http://hdl.handle.net/11449/202055 |
identifier_str_mv |
Bioenergy Research. 1939-1242 1939-1234 10.1007/s12155-020-10187-8 2-s2.0-85089989880 8251885707409794 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioenergy Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129104875094016 |