Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study

Detalhes bibliográficos
Autor(a) principal: Peres, Alessandra
Data de Publicação: 2022
Outros Autores: Branchini, Gisele, Marmett, Bruna, Nunes, Fernanda Bordignon, Romão, Pedro R.T., Olean-Oliveira, Tiago [UNESP], Minuzzi, Luciele [UNESP], Cavalcante, Mateus, Elsner, Viviane, Lira, Fabio Santos [UNESP], Dorneles, Gilson Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fphys.2022.855133
http://hdl.handle.net/11449/241377
Resumo: Aim: To evaluate the impact of exercise training plasma on in vitro prostate cancer cell viability and proliferation. Methods: PC3 prostate cancer cells were incubated with plasma obtained from young men with high and low physical fitness (PF) (high PF, n = 5; low PF, n = 5) and with the plasma collected from institutionalized older adults (n = 8) before and after multimodal exercise training. Cell viability and proliferation, mitochondria membrane polarization, reactive oxygen species (ROS) generation, and apoptosis were evaluated after the cell treatment with plasma. Systemic cytokines were evaluated in the plasma of institutionalized older adults submitted to an exercise training protocol. Results: Plasma from high-PF men lowers both cell viability and proliferation after the incubation time. PC3 cells also presented lower cell viability and diminished rates of cell proliferation after the incubation with post-training plasma samples of the older adults. The incubation of PC3 cells with post-training plasma of older adults depolarized the mitochondrial membrane potential and increased mitochondrial reactive oxygen species production. Post-training plasma did not change apoptosis or necrosis rates in the PC3 cell line. Multimodal exercise training increased the plasma levels of IL-2, IL-10, IFN-α, and FGF-1 and decreased TNF-α concentrations in institutionalized older adults. Conclusion: Adaptations in blood factors of institutionalized older adults may alter cell viability and proliferation by targeting mitochondrial ROS in a prostate cancer cell line.
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spelling Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Studyagingexercise trainingimmune responseinflammationmitochondriaprostate cancerAim: To evaluate the impact of exercise training plasma on in vitro prostate cancer cell viability and proliferation. Methods: PC3 prostate cancer cells were incubated with plasma obtained from young men with high and low physical fitness (PF) (high PF, n = 5; low PF, n = 5) and with the plasma collected from institutionalized older adults (n = 8) before and after multimodal exercise training. Cell viability and proliferation, mitochondria membrane polarization, reactive oxygen species (ROS) generation, and apoptosis were evaluated after the cell treatment with plasma. Systemic cytokines were evaluated in the plasma of institutionalized older adults submitted to an exercise training protocol. Results: Plasma from high-PF men lowers both cell viability and proliferation after the incubation time. PC3 cells also presented lower cell viability and diminished rates of cell proliferation after the incubation with post-training plasma samples of the older adults. The incubation of PC3 cells with post-training plasma of older adults depolarized the mitochondrial membrane potential and increased mitochondrial reactive oxygen species production. Post-training plasma did not change apoptosis or necrosis rates in the PC3 cell line. Multimodal exercise training increased the plasma levels of IL-2, IL-10, IFN-α, and FGF-1 and decreased TNF-α concentrations in institutionalized older adults. Conclusion: Adaptations in blood factors of institutionalized older adults may alter cell viability and proliferation by targeting mitochondrial ROS in a prostate cancer cell line.Laboratório de Imunologia Celular e Molecular Departamento de Ciências Básicas da Saúde Universidade Federal de Ciências da Saúde de Porto AlegrePrograma de Pós-graduação em Patologia Universidade Federal de Ciências da Saúde de Porto Alegre—UFCSPAExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP), Presidente PrudenteFaculty of Sports Science and Physical Education Research Center for Sports and Physical Activity University of CoimbraPrograma de Pós-graduação em Fisiologia Universidade Federal do Rio Grande do SulExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP), Presidente PrudenteUniversidade Federal de Ciências da Saúde de Porto AlegreUniversidade Federal de Ciências da Saúde de Porto Alegre—UFCSPAUniversidade Estadual Paulista (UNESP)University of CoimbraUniversidade Federal do Rio Grande do SulPeres, AlessandraBranchini, GiseleMarmett, BrunaNunes, Fernanda BordignonRomão, Pedro R.T.Olean-Oliveira, Tiago [UNESP]Minuzzi, Luciele [UNESP]Cavalcante, MateusElsner, VivianeLira, Fabio Santos [UNESP]Dorneles, Gilson Pires2023-03-01T20:59:17Z2023-03-01T20:59:17Z2022-07-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2022.855133Frontiers in Physiology, v. 13.1664-042Xhttp://hdl.handle.net/11449/24137710.3389/fphys.2022.8551332-s2.0-85134569640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-06-18T17:42:40Zoai:repositorio.unesp.br:11449/241377Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:44.773208Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
title Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
spellingShingle Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
Peres, Alessandra
aging
exercise training
immune response
inflammation
mitochondria
prostate cancer
title_short Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
title_full Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
title_fullStr Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
title_full_unstemmed Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
title_sort Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
author Peres, Alessandra
author_facet Peres, Alessandra
Branchini, Gisele
Marmett, Bruna
Nunes, Fernanda Bordignon
Romão, Pedro R.T.
Olean-Oliveira, Tiago [UNESP]
Minuzzi, Luciele [UNESP]
Cavalcante, Mateus
Elsner, Viviane
Lira, Fabio Santos [UNESP]
Dorneles, Gilson Pires
author_role author
author2 Branchini, Gisele
Marmett, Bruna
Nunes, Fernanda Bordignon
Romão, Pedro R.T.
Olean-Oliveira, Tiago [UNESP]
Minuzzi, Luciele [UNESP]
Cavalcante, Mateus
Elsner, Viviane
Lira, Fabio Santos [UNESP]
Dorneles, Gilson Pires
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Ciências da Saúde de Porto Alegre
Universidade Federal de Ciências da Saúde de Porto Alegre—UFCSPA
Universidade Estadual Paulista (UNESP)
University of Coimbra
Universidade Federal do Rio Grande do Sul
dc.contributor.author.fl_str_mv Peres, Alessandra
Branchini, Gisele
Marmett, Bruna
Nunes, Fernanda Bordignon
Romão, Pedro R.T.
Olean-Oliveira, Tiago [UNESP]
Minuzzi, Luciele [UNESP]
Cavalcante, Mateus
Elsner, Viviane
Lira, Fabio Santos [UNESP]
Dorneles, Gilson Pires
dc.subject.por.fl_str_mv aging
exercise training
immune response
inflammation
mitochondria
prostate cancer
topic aging
exercise training
immune response
inflammation
mitochondria
prostate cancer
description Aim: To evaluate the impact of exercise training plasma on in vitro prostate cancer cell viability and proliferation. Methods: PC3 prostate cancer cells were incubated with plasma obtained from young men with high and low physical fitness (PF) (high PF, n = 5; low PF, n = 5) and with the plasma collected from institutionalized older adults (n = 8) before and after multimodal exercise training. Cell viability and proliferation, mitochondria membrane polarization, reactive oxygen species (ROS) generation, and apoptosis were evaluated after the cell treatment with plasma. Systemic cytokines were evaluated in the plasma of institutionalized older adults submitted to an exercise training protocol. Results: Plasma from high-PF men lowers both cell viability and proliferation after the incubation time. PC3 cells also presented lower cell viability and diminished rates of cell proliferation after the incubation with post-training plasma samples of the older adults. The incubation of PC3 cells with post-training plasma of older adults depolarized the mitochondrial membrane potential and increased mitochondrial reactive oxygen species production. Post-training plasma did not change apoptosis or necrosis rates in the PC3 cell line. Multimodal exercise training increased the plasma levels of IL-2, IL-10, IFN-α, and FGF-1 and decreased TNF-α concentrations in institutionalized older adults. Conclusion: Adaptations in blood factors of institutionalized older adults may alter cell viability and proliferation by targeting mitochondrial ROS in a prostate cancer cell line.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-06
2023-03-01T20:59:17Z
2023-03-01T20:59:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fphys.2022.855133
Frontiers in Physiology, v. 13.
1664-042X
http://hdl.handle.net/11449/241377
10.3389/fphys.2022.855133
2-s2.0-85134569640
url http://dx.doi.org/10.3389/fphys.2022.855133
http://hdl.handle.net/11449/241377
identifier_str_mv Frontiers in Physiology, v. 13.
1664-042X
10.3389/fphys.2022.855133
2-s2.0-85134569640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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