SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.reprotox.2023.108343 http://hdl.handle.net/11449/249667 |
Resumo: | Infertility affects approximately 15% of couples of reproductive age, and 50% of the cases are directly related to men. The evaluation of male fertility is based on analyses of routine seminal parameters and the use of more advanced techniques can help identify fertility biomarkers. SP22 sperm protein is considered a biomarker in murine species since its concentration is highly correlated with sperm fertility. As the role of this protein as a biomarker is already well-established in other species, we hypothesized that this same correlation could apply to human. Thus, the present study aimed to investigate possible correlations between SP22 concentration and sperm parameters in fertile and infertile men. For this, a study was carried out on 21 volunteers’ seminal samples who were grouped according to fertility as fertile (n = 10) or infertile (n = 11). Conventional and functional sperm analyses, membrane protein extraction, quantification and immunolocalization of SP22 were performed. The infertile volunteers showed an increase in the percentage of sperm with abnormalities in head morphology and a decrease in the percentage of sperm with intact plasma membrane and damaged acrosomal membrane. Serum concentration of the hormone SHBG was also decreased in infertile volunteers. The damage to the plasma membrane was positively correlated with the superoxide anion production. Although none of the functional parameters were correlated with SP22 concentration, type D sperm motility was negatively correlated and type A+B sperm motility was positively correlated. This preliminary study opens new paths in the characterization of SP22 as a non-invasive biomarker for predicting fertility/infertility. |
id |
UNSP_c3bc27dd07c9c17ad31a2c365221991a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/249667 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary studyFertility biomarkerHuman spermMale infertilityReproductive toxicitySP22Sperm proteinInfertility affects approximately 15% of couples of reproductive age, and 50% of the cases are directly related to men. The evaluation of male fertility is based on analyses of routine seminal parameters and the use of more advanced techniques can help identify fertility biomarkers. SP22 sperm protein is considered a biomarker in murine species since its concentration is highly correlated with sperm fertility. As the role of this protein as a biomarker is already well-established in other species, we hypothesized that this same correlation could apply to human. Thus, the present study aimed to investigate possible correlations between SP22 concentration and sperm parameters in fertile and infertile men. For this, a study was carried out on 21 volunteers’ seminal samples who were grouped according to fertility as fertile (n = 10) or infertile (n = 11). Conventional and functional sperm analyses, membrane protein extraction, quantification and immunolocalization of SP22 were performed. The infertile volunteers showed an increase in the percentage of sperm with abnormalities in head morphology and a decrease in the percentage of sperm with intact plasma membrane and damaged acrosomal membrane. Serum concentration of the hormone SHBG was also decreased in infertile volunteers. The damage to the plasma membrane was positively correlated with the superoxide anion production. Although none of the functional parameters were correlated with SP22 concentration, type D sperm motility was negatively correlated and type A+B sperm motility was positively correlated. This preliminary study opens new paths in the characterization of SP22 as a non-invasive biomarker for predicting fertility/infertility.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Biosciences Department of Structural and Functional Biology Laboratory of Reproductive and Developmental Biology and Toxicology São Paulo State University (UNESP), São PauloNational Health and Environmental Effects Research Laboratory Office of Research and Development US Environmental Protection Agency Research Triangle ParkInstitute of Biosciences Department of Structural and Functional Biology Laboratory of Reproductive and Developmental Biology and Toxicology São Paulo State University (UNESP), São PauloFAPESP: FAPESP 2015/13941-3Universidade Estadual Paulista (UNESP)Research Triangle ParkRosa, Josiane de Lima [UNESP]Figueiredo, Thamiris Moreira [UNESP]Barros, Jorge Willian Franco [UNESP]dos Santos, Driéle Bretone [UNESP]de Deus, Ramão Souza [UNESP]Borges, Cibele Santos [UNESP]Kempinas, Wilma De Grava [UNESP]Klinefelter, Gary Robert2023-07-29T16:05:57Z2023-07-29T16:05:57Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.reprotox.2023.108343Reproductive Toxicology, v. 117.1873-17080890-6238http://hdl.handle.net/11449/24966710.1016/j.reprotox.2023.1083432-s2.0-85148342265Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Toxicologyinfo:eu-repo/semantics/openAccess2023-07-29T16:05:57Zoai:repositorio.unesp.br:11449/249667Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:08:49.307285Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
title |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
spellingShingle |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study Rosa, Josiane de Lima [UNESP] Fertility biomarker Human sperm Male infertility Reproductive toxicity SP22 Sperm protein |
title_short |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
title_full |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
title_fullStr |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
title_full_unstemmed |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
title_sort |
SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study |
author |
Rosa, Josiane de Lima [UNESP] |
author_facet |
Rosa, Josiane de Lima [UNESP] Figueiredo, Thamiris Moreira [UNESP] Barros, Jorge Willian Franco [UNESP] dos Santos, Driéle Bretone [UNESP] de Deus, Ramão Souza [UNESP] Borges, Cibele Santos [UNESP] Kempinas, Wilma De Grava [UNESP] Klinefelter, Gary Robert |
author_role |
author |
author2 |
Figueiredo, Thamiris Moreira [UNESP] Barros, Jorge Willian Franco [UNESP] dos Santos, Driéle Bretone [UNESP] de Deus, Ramão Souza [UNESP] Borges, Cibele Santos [UNESP] Kempinas, Wilma De Grava [UNESP] Klinefelter, Gary Robert |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Research Triangle Park |
dc.contributor.author.fl_str_mv |
Rosa, Josiane de Lima [UNESP] Figueiredo, Thamiris Moreira [UNESP] Barros, Jorge Willian Franco [UNESP] dos Santos, Driéle Bretone [UNESP] de Deus, Ramão Souza [UNESP] Borges, Cibele Santos [UNESP] Kempinas, Wilma De Grava [UNESP] Klinefelter, Gary Robert |
dc.subject.por.fl_str_mv |
Fertility biomarker Human sperm Male infertility Reproductive toxicity SP22 Sperm protein |
topic |
Fertility biomarker Human sperm Male infertility Reproductive toxicity SP22 Sperm protein |
description |
Infertility affects approximately 15% of couples of reproductive age, and 50% of the cases are directly related to men. The evaluation of male fertility is based on analyses of routine seminal parameters and the use of more advanced techniques can help identify fertility biomarkers. SP22 sperm protein is considered a biomarker in murine species since its concentration is highly correlated with sperm fertility. As the role of this protein as a biomarker is already well-established in other species, we hypothesized that this same correlation could apply to human. Thus, the present study aimed to investigate possible correlations between SP22 concentration and sperm parameters in fertile and infertile men. For this, a study was carried out on 21 volunteers’ seminal samples who were grouped according to fertility as fertile (n = 10) or infertile (n = 11). Conventional and functional sperm analyses, membrane protein extraction, quantification and immunolocalization of SP22 were performed. The infertile volunteers showed an increase in the percentage of sperm with abnormalities in head morphology and a decrease in the percentage of sperm with intact plasma membrane and damaged acrosomal membrane. Serum concentration of the hormone SHBG was also decreased in infertile volunteers. The damage to the plasma membrane was positively correlated with the superoxide anion production. Although none of the functional parameters were correlated with SP22 concentration, type D sperm motility was negatively correlated and type A+B sperm motility was positively correlated. This preliminary study opens new paths in the characterization of SP22 as a non-invasive biomarker for predicting fertility/infertility. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:05:57Z 2023-07-29T16:05:57Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.reprotox.2023.108343 Reproductive Toxicology, v. 117. 1873-1708 0890-6238 http://hdl.handle.net/11449/249667 10.1016/j.reprotox.2023.108343 2-s2.0-85148342265 |
url |
http://dx.doi.org/10.1016/j.reprotox.2023.108343 http://hdl.handle.net/11449/249667 |
identifier_str_mv |
Reproductive Toxicology, v. 117. 1873-1708 0890-6238 10.1016/j.reprotox.2023.108343 2-s2.0-85148342265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128761623740416 |