The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0716-97602013000100008 http://hdl.handle.net/11449/75674 |
Resumo: | The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (FST) proved to be high among the population of Marmeleiro-PR and the three populations of SP (FST > 0.288); genetic dissimilarity was related to the geographical distance. The ISSR proved to be efficient and useful molecular markers in comparison with other markers most widely used for preliminary diagnosis of genetic diversity in populations of amphibians, and could be applied as a tool for future conservation projects, since they could identify potential ESUs and influence decisions on the preservation of fragments. |
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The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)ESUFSTGene flowGenetic structureMolecular markerThe greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (FST) proved to be high among the population of Marmeleiro-PR and the three populations of SP (FST > 0.288); genetic dissimilarity was related to the geographical distance. The ISSR proved to be efficient and useful molecular markers in comparison with other markers most widely used for preliminary diagnosis of genetic diversity in populations of amphibians, and could be applied as a tool for future conservation projects, since they could identify potential ESUs and influence decisions on the preservation of fragments.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp) Departamento de Biologia, Rua Cristóvão Colombo, 2265 - Jardim Nazareth, CEP 15054-000, Sao Jose do Rio Preto, Sao PauloUniversidade Tecnológica Federal do Paraná (UTFPR) Ciências Biológicas, Estrada para Boa Esperanca, Comunidade Sao Cristovao, CEP 85660-000, Dois Vizinhos, ParanáUniversidade Estadual do Oeste do Paraná (Unioeste) Centro de Ciências Biológicas e a Saúde, Rua Universitária, 2069 - Jardim Universitario, CEP 85819-110, Cascavel, ParanáUniversidade Estadual Paulista (Unesp) Departamento de Biologia, Rua Cristóvão Colombo, 2265 - Jardim Nazareth, CEP 15054-000, Sao Jose do Rio Preto, Sao PauloCNPq: 474596/2010-9Universidade Estadual Paulista (Unesp)Ciências BiológicasCentro de Ciências Biológicas e a SaúdeMoresco, Rafaela M. [UNESP]Maniglia, Thiago C.De Oliveira, Classius [UNESP]Margarido, Vladimir P.2014-05-27T11:29:42Z2014-05-27T11:29:42Z2013-06-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article53-57application/pdfhttp://dx.doi.org/10.4067/S0716-97602013000100008Biological Research, v. 46, n. 1, p. 53-57, 2013.0716-97600717-6287http://hdl.handle.net/11449/7567410.4067/S0716-97602013000100008S0716-97602013000100008WOS:0003175413000082-s2.0-848788896802-s2.0-84878889680.pdf57318566502178590000-0002-2029-7900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Research2.3570,6540,654info:eu-repo/semantics/openAccess2023-10-27T06:05:38Zoai:repositorio.unesp.br:11449/75674Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:04:51.268727Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
title |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
spellingShingle |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) Moresco, Rafaela M. [UNESP] ESU FST Gene flow Genetic structure Molecular marker |
title_short |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
title_full |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
title_fullStr |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
title_full_unstemmed |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
title_sort |
The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: Preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) |
author |
Moresco, Rafaela M. [UNESP] |
author_facet |
Moresco, Rafaela M. [UNESP] Maniglia, Thiago C. De Oliveira, Classius [UNESP] Margarido, Vladimir P. |
author_role |
author |
author2 |
Maniglia, Thiago C. De Oliveira, Classius [UNESP] Margarido, Vladimir P. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ciências Biológicas Centro de Ciências Biológicas e a Saúde |
dc.contributor.author.fl_str_mv |
Moresco, Rafaela M. [UNESP] Maniglia, Thiago C. De Oliveira, Classius [UNESP] Margarido, Vladimir P. |
dc.subject.por.fl_str_mv |
ESU FST Gene flow Genetic structure Molecular marker |
topic |
ESU FST Gene flow Genetic structure Molecular marker |
description |
The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (FST) proved to be high among the population of Marmeleiro-PR and the three populations of SP (FST > 0.288); genetic dissimilarity was related to the geographical distance. The ISSR proved to be efficient and useful molecular markers in comparison with other markers most widely used for preliminary diagnosis of genetic diversity in populations of amphibians, and could be applied as a tool for future conservation projects, since they could identify potential ESUs and influence decisions on the preservation of fragments. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-17 2014-05-27T11:29:42Z 2014-05-27T11:29:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0716-97602013000100008 Biological Research, v. 46, n. 1, p. 53-57, 2013. 0716-9760 0717-6287 http://hdl.handle.net/11449/75674 10.4067/S0716-97602013000100008 S0716-97602013000100008 WOS:000317541300008 2-s2.0-84878889680 2-s2.0-84878889680.pdf 5731856650217859 0000-0002-2029-7900 |
url |
http://dx.doi.org/10.4067/S0716-97602013000100008 http://hdl.handle.net/11449/75674 |
identifier_str_mv |
Biological Research, v. 46, n. 1, p. 53-57, 2013. 0716-9760 0717-6287 10.4067/S0716-97602013000100008 S0716-97602013000100008 WOS:000317541300008 2-s2.0-84878889680 2-s2.0-84878889680.pdf 5731856650217859 0000-0002-2029-7900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Research 2.357 0,654 0,654 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
53-57 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128605087072256 |