The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2020/3026893 http://hdl.handle.net/11449/205295 |
Resumo: | There is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano®). To better understand the biological behavior of osteoblasts grown on HAnano® surface, the set of data was compared with SLActive®, a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano® displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive®-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression of integrin β1, Fak, and Src, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating of Cdk2, Cdk4, and Cdk6 genes) in response to HAnano®. Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activating Runx2, Osterix, and Alp genes. Although both surfaces promoted Rankl gene expression, Opg gene expression was higher in SLActive® and this difference reflected on the Rankl/Opg ratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano® and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration. |
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The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic SurfacesThere is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano®). To better understand the biological behavior of osteoblasts grown on HAnano® surface, the set of data was compared with SLActive®, a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano® displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive®-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression of integrin β1, Fak, and Src, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating of Cdk2, Cdk4, and Cdk6 genes) in response to HAnano®. Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activating Runx2, Osterix, and Alp genes. Although both surfaces promoted Rankl gene expression, Opg gene expression was higher in SLActive® and this difference reflected on the Rankl/Opg ratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano® and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration.Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State UniversitySchool of Dentistry University of TaubatéProgram in Environmental and Experimental Pathology Paulista UniversityLab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State UniversityUniversidade Estadual Paulista (Unesp)University of TaubatéPaulista UniversityDa Silva, Rodrigo A. [UNESP]Da Silva Feltran, Geórgia [UNESP]Ferreira, Marcel Rodrigues [UNESP]Wood, Patrícia Fretes [UNESP]Bezerra, Fabio [UNESP]Zambuzzi, Willian F. [UNESP]2021-06-25T10:13:01Z2021-06-25T10:13:01Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2020/3026893BioMed Research International, v. 2020.2314-61412314-6133http://hdl.handle.net/11449/20529510.1155/2020/30268932-s2.0-85092463358Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioMed Research Internationalinfo:eu-repo/semantics/openAccess2021-10-23T12:31:17Zoai:repositorio.unesp.br:11449/205295Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:49.808186Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
title |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
spellingShingle |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces Da Silva, Rodrigo A. [UNESP] |
title_short |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
title_full |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
title_fullStr |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
title_full_unstemmed |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
title_sort |
The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An in Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces |
author |
Da Silva, Rodrigo A. [UNESP] |
author_facet |
Da Silva, Rodrigo A. [UNESP] Da Silva Feltran, Geórgia [UNESP] Ferreira, Marcel Rodrigues [UNESP] Wood, Patrícia Fretes [UNESP] Bezerra, Fabio [UNESP] Zambuzzi, Willian F. [UNESP] |
author_role |
author |
author2 |
Da Silva Feltran, Geórgia [UNESP] Ferreira, Marcel Rodrigues [UNESP] Wood, Patrícia Fretes [UNESP] Bezerra, Fabio [UNESP] Zambuzzi, Willian F. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Taubaté Paulista University |
dc.contributor.author.fl_str_mv |
Da Silva, Rodrigo A. [UNESP] Da Silva Feltran, Geórgia [UNESP] Ferreira, Marcel Rodrigues [UNESP] Wood, Patrícia Fretes [UNESP] Bezerra, Fabio [UNESP] Zambuzzi, Willian F. [UNESP] |
description |
There is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano®). To better understand the biological behavior of osteoblasts grown on HAnano® surface, the set of data was compared with SLActive®, a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano® displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive®-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression of integrin β1, Fak, and Src, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating of Cdk2, Cdk4, and Cdk6 genes) in response to HAnano®. Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activating Runx2, Osterix, and Alp genes. Although both surfaces promoted Rankl gene expression, Opg gene expression was higher in SLActive® and this difference reflected on the Rankl/Opg ratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano® and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:13:01Z 2021-06-25T10:13:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2020/3026893 BioMed Research International, v. 2020. 2314-6141 2314-6133 http://hdl.handle.net/11449/205295 10.1155/2020/3026893 2-s2.0-85092463358 |
url |
http://dx.doi.org/10.1155/2020/3026893 http://hdl.handle.net/11449/205295 |
identifier_str_mv |
BioMed Research International, v. 2020. 2314-6141 2314-6133 10.1155/2020/3026893 2-s2.0-85092463358 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BioMed Research International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128832183468032 |