Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity

Detalhes bibliográficos
Autor(a) principal: dos Santos, Verônica Ribeiro
Data de Publicação: 2023
Outros Autores: Vera, Samara Domingues, de Cena, Gabrielle Lupeti, Silva, Adrielle de Paula, Lemes, Ana Paula, da Conceição, Kátia, Tada, Dayane Batista, Borges, Alexandre Luiz Souto [UNESP], Trichês, Eliandra de Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0104-1428.20220088
http://hdl.handle.net/11449/248826
Resumo: Aiming to produce an antimicrobial dressing for wound healing applications, in this work Tea Tree oil (TTO) was incorporated into PHBV nanofibers by absorption. It was observed increase in the nanofiber diameter due to 5% TTO absorption efficiency, which also led to a 54% decrease in the contact angle. The releasing assay indicates a 6.8% oil release in the first 24 h – being probably the oil deposited at the polymer surface – followed by a minimal release at 48 h. The set of antimicrobial assays performed suggests the incorporation of TTO optimized the antimicrobial activity of the polymer for E. coli and C. albicans, while against S. aureus no significant difference was observed. The MTT assay showed no cytotoxicity of PHBV, but the incubation of L929 fibroblast cells with PHBV-TTO reduced cell viability. Overall, the PHBV nanofibers containing TTO present great potential as an antimicrobial dressing.
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spelling Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activityantimicrobial activitynanofiberPHBVtea tree oilwound healingAiming to produce an antimicrobial dressing for wound healing applications, in this work Tea Tree oil (TTO) was incorporated into PHBV nanofibers by absorption. It was observed increase in the nanofiber diameter due to 5% TTO absorption efficiency, which also led to a 54% decrease in the contact angle. The releasing assay indicates a 6.8% oil release in the first 24 h – being probably the oil deposited at the polymer surface – followed by a minimal release at 48 h. The set of antimicrobial assays performed suggests the incorporation of TTO optimized the antimicrobial activity of the polymer for E. coli and C. albicans, while against S. aureus no significant difference was observed. The MTT assay showed no cytotoxicity of PHBV, but the incubation of L929 fibroblast cells with PHBV-TTO reduced cell viability. Overall, the PHBV nanofibers containing TTO present great potential as an antimicrobial dressing.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Biocerâmicas Instituto de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESP, SPLaboratório de Bioquímica de Pepitídeos Instituto de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESPLaboratório de Nanomateriais e Nanotoxicologia Instituto de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESP, SPLaboratório de Tecnologia em Polímeros e Biopolímeros Instituto de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESP, SPDepartamento de Materiais Dentários e Prostodontia Instituto de Ciência e Tecnologia Universidade Estadual Paulista – UNESP, SPDepartamento de Materiais Dentários e Prostodontia Instituto de Ciência e Tecnologia Universidade Estadual Paulista – UNESP, SPFAPESP: 2019-19594-4FAPESP: 2019/10877-3FAPESP: 2020/04455-6Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (UNESP)dos Santos, Verônica RibeiroVera, Samara Dominguesde Cena, Gabrielle LupetiSilva, Adrielle de PaulaLemes, Ana Paulada Conceição, KátiaTada, Dayane BatistaBorges, Alexandre Luiz Souto [UNESP]Trichês, Eliandra de Sousa2023-07-29T13:54:47Z2023-07-29T13:54:47Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0104-1428.20220088Polimeros, v. 33, n. 1, 2023.1678-51690104-1428http://hdl.handle.net/11449/24882610.1590/0104-1428.202200882-s2.0-85159169798Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolimerosinfo:eu-repo/semantics/openAccess2023-07-29T13:54:47Zoai:repositorio.unesp.br:11449/248826Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:47:20.906705Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
title Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
spellingShingle Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
dos Santos, Verônica Ribeiro
antimicrobial activity
nanofiber
PHBV
tea tree oil
wound healing
title_short Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
title_full Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
title_fullStr Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
title_full_unstemmed Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
title_sort Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity
author dos Santos, Verônica Ribeiro
author_facet dos Santos, Verônica Ribeiro
Vera, Samara Domingues
de Cena, Gabrielle Lupeti
Silva, Adrielle de Paula
Lemes, Ana Paula
da Conceição, Kátia
Tada, Dayane Batista
Borges, Alexandre Luiz Souto [UNESP]
Trichês, Eliandra de Sousa
author_role author
author2 Vera, Samara Domingues
de Cena, Gabrielle Lupeti
Silva, Adrielle de Paula
Lemes, Ana Paula
da Conceição, Kátia
Tada, Dayane Batista
Borges, Alexandre Luiz Souto [UNESP]
Trichês, Eliandra de Sousa
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv dos Santos, Verônica Ribeiro
Vera, Samara Domingues
de Cena, Gabrielle Lupeti
Silva, Adrielle de Paula
Lemes, Ana Paula
da Conceição, Kátia
Tada, Dayane Batista
Borges, Alexandre Luiz Souto [UNESP]
Trichês, Eliandra de Sousa
dc.subject.por.fl_str_mv antimicrobial activity
nanofiber
PHBV
tea tree oil
wound healing
topic antimicrobial activity
nanofiber
PHBV
tea tree oil
wound healing
description Aiming to produce an antimicrobial dressing for wound healing applications, in this work Tea Tree oil (TTO) was incorporated into PHBV nanofibers by absorption. It was observed increase in the nanofiber diameter due to 5% TTO absorption efficiency, which also led to a 54% decrease in the contact angle. The releasing assay indicates a 6.8% oil release in the first 24 h – being probably the oil deposited at the polymer surface – followed by a minimal release at 48 h. The set of antimicrobial assays performed suggests the incorporation of TTO optimized the antimicrobial activity of the polymer for E. coli and C. albicans, while against S. aureus no significant difference was observed. The MTT assay showed no cytotoxicity of PHBV, but the incubation of L929 fibroblast cells with PHBV-TTO reduced cell viability. Overall, the PHBV nanofibers containing TTO present great potential as an antimicrobial dressing.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:54:47Z
2023-07-29T13:54:47Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0104-1428.20220088
Polimeros, v. 33, n. 1, 2023.
1678-5169
0104-1428
http://hdl.handle.net/11449/248826
10.1590/0104-1428.20220088
2-s2.0-85159169798
url http://dx.doi.org/10.1590/0104-1428.20220088
http://hdl.handle.net/11449/248826
identifier_str_mv Polimeros, v. 33, n. 1, 2023.
1678-5169
0104-1428
10.1590/0104-1428.20220088
2-s2.0-85159169798
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Polimeros
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129551942811648