Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/imb.12684 http://hdl.handle.net/11449/208230 |
Resumo: | Termites are well recognized by their complex development trajectories, involving dynamic differentiation process between non-reproductive castes, workers and soldiers. These insects are associated with endosymbiotic microorganisms, which help in lignocellulose digestion and nitrogen metabolism. Aiming to identify genes harbouring biotechnological potential, we analyzed workers and soldiers RNA-Seq data of three neotropical termites: Heterotermes tenuis (Isoptera: Rhinotermitidae), Velocitermes heteropterus (Isoptera: Termitidae) and Cornitermes cumulans (Isoptera: Termitidae). We observed differences in the microbiota associated with each termite family, and found protists' genes in both Termitidae species. We found an opposite pattern of caste-biased gene expression between H. tenuis and the termitids studied. Moreover, the two termitids are considerably different concerning the number of differentially expressed genes (DEGs). Functional annotation indicated considerable differences in caste-biased gene content between V. heteropterus and C. cumulans, even though they share similar diet and biological niche. Among the most DEGs, we highlighted those involved in caste differentiation and cellulose digestion, which are attractive targets for studying more efficient technologies for termite control, biomass digestion and other biotechnological applications. |
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Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targetscellulolytic genesmeta-transcriptomesRhinotermitidaeTermitidaeTermites are well recognized by their complex development trajectories, involving dynamic differentiation process between non-reproductive castes, workers and soldiers. These insects are associated with endosymbiotic microorganisms, which help in lignocellulose digestion and nitrogen metabolism. Aiming to identify genes harbouring biotechnological potential, we analyzed workers and soldiers RNA-Seq data of three neotropical termites: Heterotermes tenuis (Isoptera: Rhinotermitidae), Velocitermes heteropterus (Isoptera: Termitidae) and Cornitermes cumulans (Isoptera: Termitidae). We observed differences in the microbiota associated with each termite family, and found protists' genes in both Termitidae species. We found an opposite pattern of caste-biased gene expression between H. tenuis and the termitids studied. Moreover, the two termitids are considerably different concerning the number of differentially expressed genes (DEGs). Functional annotation indicated considerable differences in caste-biased gene content between V. heteropterus and C. cumulans, even though they share similar diet and biological niche. Among the most DEGs, we highlighted those involved in caste differentiation and cellulose digestion, which are attractive targets for studying more efficient technologies for termite control, biomass digestion and other biotechnological applications.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Genética e Evolução Centro de Ciências Biológicas e da Saúde Universidade Federal de São CarlosDepartamento de Genética Evolução Microbiologia e Imunologia Instituto de Biologia Universidade Estadual de CampinasLaboratório de Cupins Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) campus de Rio ClaroLaboratório de Cupins Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) campus de Rio ClaroCNPq: CNPq: 139192/2015-8CAPES: DS-CAPES 88882.426720/2019-01CAPES: DS-CAPES 88882.426727/2019-01CAPES: Finance Code 001CNPq: PIBIC: 2743Universidade Federal de São Carlos (UFSCar)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Campanini, E. B.Pedrino, M.Martins, L. A.Athaide Neta, O. S.Carazzolle, M. F.Ciancaglini, I.Malavazi, I.Costa-Leonardo, A. M. [UNESP]de Melo Freire, C. C.Nunes, F. M.F.da Cunha, A. F.2021-06-25T11:08:38Z2021-06-25T11:08:38Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article152-164http://dx.doi.org/10.1111/imb.12684Insect Molecular Biology, v. 30, n. 2, p. 152-164, 2021.1365-25830962-1075http://hdl.handle.net/11449/20823010.1111/imb.126842-s2.0-85097499770Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInsect Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-23T18:56:55Zoai:repositorio.unesp.br:11449/208230Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:18.461090Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
title |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
spellingShingle |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets Campanini, E. B. cellulolytic genes meta-transcriptomes Rhinotermitidae Termitidae |
title_short |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
title_full |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
title_fullStr |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
title_full_unstemmed |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
title_sort |
Expression profiles of neotropical termites reveal microbiota-associated, caste-biased genes and biotechnological targets |
author |
Campanini, E. B. |
author_facet |
Campanini, E. B. Pedrino, M. Martins, L. A. Athaide Neta, O. S. Carazzolle, M. F. Ciancaglini, I. Malavazi, I. Costa-Leonardo, A. M. [UNESP] de Melo Freire, C. C. Nunes, F. M.F. da Cunha, A. F. |
author_role |
author |
author2 |
Pedrino, M. Martins, L. A. Athaide Neta, O. S. Carazzolle, M. F. Ciancaglini, I. Malavazi, I. Costa-Leonardo, A. M. [UNESP] de Melo Freire, C. C. Nunes, F. M.F. da Cunha, A. F. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Campanini, E. B. Pedrino, M. Martins, L. A. Athaide Neta, O. S. Carazzolle, M. F. Ciancaglini, I. Malavazi, I. Costa-Leonardo, A. M. [UNESP] de Melo Freire, C. C. Nunes, F. M.F. da Cunha, A. F. |
dc.subject.por.fl_str_mv |
cellulolytic genes meta-transcriptomes Rhinotermitidae Termitidae |
topic |
cellulolytic genes meta-transcriptomes Rhinotermitidae Termitidae |
description |
Termites are well recognized by their complex development trajectories, involving dynamic differentiation process between non-reproductive castes, workers and soldiers. These insects are associated with endosymbiotic microorganisms, which help in lignocellulose digestion and nitrogen metabolism. Aiming to identify genes harbouring biotechnological potential, we analyzed workers and soldiers RNA-Seq data of three neotropical termites: Heterotermes tenuis (Isoptera: Rhinotermitidae), Velocitermes heteropterus (Isoptera: Termitidae) and Cornitermes cumulans (Isoptera: Termitidae). We observed differences in the microbiota associated with each termite family, and found protists' genes in both Termitidae species. We found an opposite pattern of caste-biased gene expression between H. tenuis and the termitids studied. Moreover, the two termitids are considerably different concerning the number of differentially expressed genes (DEGs). Functional annotation indicated considerable differences in caste-biased gene content between V. heteropterus and C. cumulans, even though they share similar diet and biological niche. Among the most DEGs, we highlighted those involved in caste differentiation and cellulose digestion, which are attractive targets for studying more efficient technologies for termite control, biomass digestion and other biotechnological applications. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:08:38Z 2021-06-25T11:08:38Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/imb.12684 Insect Molecular Biology, v. 30, n. 2, p. 152-164, 2021. 1365-2583 0962-1075 http://hdl.handle.net/11449/208230 10.1111/imb.12684 2-s2.0-85097499770 |
url |
http://dx.doi.org/10.1111/imb.12684 http://hdl.handle.net/11449/208230 |
identifier_str_mv |
Insect Molecular Biology, v. 30, n. 2, p. 152-164, 2021. 1365-2583 0962-1075 10.1111/imb.12684 2-s2.0-85097499770 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Insect Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
152-164 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129334869753856 |