Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts

• Autor(a) principal: Hata Viveiros, Magda Massae [UNESP]
• Data de Publicação: 2022
• Outros Autores: Silva, Márcia Guimarães [UNESP], da Costa, José Galberto Martins, de Oliveira, Anselmo Gomes [UNESP], Rubio, Carolina [UNESP], Padovani, Carlos Roberto [UNESP], Rainho, Cláudia Aparecida [UNESP], Schellini, Silvana Artioli [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.18240/ijo.2022.12.02; http://hdl.handle.net/11449/247999
• topic: cytokines; fibroblasts; pterygium; α-humulene; β-caryophyllene

• AIM: To investigate the anti-inflammatory effects of the sesquiterpenes α-humulene and β-caryophyllene on pterygium fibroblasts. • METHODS: Primary cultures of pterygium fibroblasts were established. Third passage pterygium fibroblasts were exposed to α-humulene and β-caryophyllene separately and together. The cell viability was assessed by 3-(4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide (MTT) assay at 12, 24, 48, and 72h after exposure. The levels of the inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and IL-10 in the conditioned culture medium were assessed by enzyme-linked immunosorbent assay (ELISA) at 12, 24 and 48h after exposure. Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks. • RESulTS: The 25 μmol/L β-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure (P=0.041). The levels of IL-1β, IL-8, IL-10, and TNF-α were very low and had no statistically significant variations after exposure to α-humulene, β-caryophyllene, or both compounds together. • COnCluSIOn:Theexposureto25μmol/Lofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h. This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.