Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1414-431X2020e11073 http://hdl.handle.net/11449/210370 |
Resumo: | The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells. |
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Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cellsOxidative stressPlacental explantsPreeclampsiaVitamin DThe study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Swedish Research CouncilSwedish Heart and Lung FoundationNovoNordiskUniv Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilKarolinska Inst, Dept Physiol & Pharmacol, Stockholm, SwedenUniv Estadual Paulista, Inst Biociencias, Dept Biofis & Farmacol, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biofis & Farmacol, Botucatu, SP, BrazilFAPESP: 2016/23452-2FAPESP: 2019/00234-8Swedish Research Council: 2016-01381Swedish Research Council: 2020-01645Swedish Heart and Lung Foundation: 20170124Swedish Heart and Lung Foundation: 20180568NovoNordisk: 0055026Assoc Bras Divulg CientificaUniversidade Estadual Paulista (Unesp)Karolinska InstNunes, P. R. [UNESP]Gomes, V. J. [UNESP]Sandrim, V. C. [UNESP]Peracoli, J. C. [UNESP]Peracoli, M. T. S. [UNESP]Carlstrom, M.2021-06-25T15:06:18Z2021-06-25T15:06:18Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1590/1414-431X2020e11073Brazilian Journal Of Medical And Biological Research. Ribeirao Preto: Assoc Bras Divulg Cientifica, v. 54, n. 8, 10 p., 2021.0100-879Xhttp://hdl.handle.net/11449/21037010.1590/1414-431X2020e11073S0100-879X2021000800604WOS:000655049800001S0100-879X2021000800604.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Medical And Biological Researchinfo:eu-repo/semantics/openAccess2024-08-16T14:06:43Zoai:repositorio.unesp.br:11449/210370Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
title |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
spellingShingle |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells Nunes, P. R. [UNESP] Oxidative stress Placental explants Preeclampsia Vitamin D |
title_short |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
title_full |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
title_fullStr |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
title_full_unstemmed |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
title_sort |
Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells |
author |
Nunes, P. R. [UNESP] |
author_facet |
Nunes, P. R. [UNESP] Gomes, V. J. [UNESP] Sandrim, V. C. [UNESP] Peracoli, J. C. [UNESP] Peracoli, M. T. S. [UNESP] Carlstrom, M. |
author_role |
author |
author2 |
Gomes, V. J. [UNESP] Sandrim, V. C. [UNESP] Peracoli, J. C. [UNESP] Peracoli, M. T. S. [UNESP] Carlstrom, M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Karolinska Inst |
dc.contributor.author.fl_str_mv |
Nunes, P. R. [UNESP] Gomes, V. J. [UNESP] Sandrim, V. C. [UNESP] Peracoli, J. C. [UNESP] Peracoli, M. T. S. [UNESP] Carlstrom, M. |
dc.subject.por.fl_str_mv |
Oxidative stress Placental explants Preeclampsia Vitamin D |
topic |
Oxidative stress Placental explants Preeclampsia Vitamin D |
description |
The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:06:18Z 2021-06-25T15:06:18Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X2020e11073 Brazilian Journal Of Medical And Biological Research. Ribeirao Preto: Assoc Bras Divulg Cientifica, v. 54, n. 8, 10 p., 2021. 0100-879X http://hdl.handle.net/11449/210370 10.1590/1414-431X2020e11073 S0100-879X2021000800604 WOS:000655049800001 S0100-879X2021000800604.pdf |
url |
http://dx.doi.org/10.1590/1414-431X2020e11073 http://hdl.handle.net/11449/210370 |
identifier_str_mv |
Brazilian Journal Of Medical And Biological Research. Ribeirao Preto: Assoc Bras Divulg Cientifica, v. 54, n. 8, 10 p., 2021. 0100-879X 10.1590/1414-431X2020e11073 S0100-879X2021000800604 WOS:000655049800001 S0100-879X2021000800604.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Medical And Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
publisher.none.fl_str_mv |
Assoc Bras Divulg Cientifica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128125165371392 |