Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-021-14974-4 http://hdl.handle.net/11449/229033 |
Resumo: | The sensitivity of anuran to the effects of habitat destruction and contamination has led to a preoccupying global decline in their populations. Morphological biomarkers such as micronuclei and other erythrocyte nuclear abnormalities (ENAs), as well as the occurrence of hepatic melanin, can be used to evaluate the effects of habitat impacts. In the present study, these two parameters were combined for the in situ assessment of the effects of soybean cultivation on the grassfrog, Leptodactylus fuscus. Specimens were also collected from a protected area to provide a reference site (non-agricultural environment). The frequency of some of the nuclear abnormalities in the animals from the soybean plantation was much higher than that recorded at the reference site, in particular micronuclei, which were 3.6 times more frequent in the plantation, lobulated nuclei (3.4 times more frequent), and reniform nuclei, which were four times more common than at the reference site. The combined analysis of all the ENAs together also revealed a frequency approximately 1.4 times higher in the animals from the soybean plantation, in comparison with the protected area. Smaller areas of hepatic melanin were observed in the specimens from the soybean plantation. These results provide further evidence of the sensitivity of anurans to habitat impacts and indicate that animals found in soybean plantations are susceptible to systematic alterations of their cells. |
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Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savannaAgricultureAnuransErythrocytic nuclear abnormalitiesHepatic melaninMicronucleusSoybean cultivationThe sensitivity of anuran to the effects of habitat destruction and contamination has led to a preoccupying global decline in their populations. Morphological biomarkers such as micronuclei and other erythrocyte nuclear abnormalities (ENAs), as well as the occurrence of hepatic melanin, can be used to evaluate the effects of habitat impacts. In the present study, these two parameters were combined for the in situ assessment of the effects of soybean cultivation on the grassfrog, Leptodactylus fuscus. Specimens were also collected from a protected area to provide a reference site (non-agricultural environment). The frequency of some of the nuclear abnormalities in the animals from the soybean plantation was much higher than that recorded at the reference site, in particular micronuclei, which were 3.6 times more frequent in the plantation, lobulated nuclei (3.4 times more frequent), and reniform nuclei, which were four times more common than at the reference site. The combined analysis of all the ENAs together also revealed a frequency approximately 1.4 times higher in the animals from the soybean plantation, in comparison with the protected area. Smaller areas of hepatic melanin were observed in the specimens from the soybean plantation. These results provide further evidence of the sensitivity of anurans to habitat impacts and indicate that animals found in soybean plantations are susceptible to systematic alterations of their cells.Laboratory of Ecotoxicology and Animal Systematics (EcotoxSA) Goiano Federal Institute, Rio VerdeDepartment of Biology Paulista State University “Júlio de Mesquita Filho”, São José do Rio PretoMutagenesis Laboratory Institute of Biological Sciences Federal University of Goiás, GoiâniaUniversity of Rio Verde, Rio VerdeInstitute of Biosciences Federal University of Mato Grosso do Sul, Campo GrandeDepartment of Biology Paulista State University “Júlio de Mesquita Filho”, São José do Rio PretoGoiano Federal InstituteUniversidade Estadual Paulista (UNESP)Universidade Federal de Goiás (UFG)University of Rio VerdeFederal University of Mato Grosso do SulAssis, Rhayane Alves [UNESP]Rezende, Wadson Rodrigues [UNESP]dos Santos, Cirley Gomes AraújoBenvindo-Souza, MarcelinoAmorim, Nathan Pereira LimaBorges, Rinneu EliasFranco-Belussi, Lilian [UNESP]De Oliveira, Classius [UNESP]de Souza Santos, Lia Raquel2022-04-29T08:30:03Z2022-04-29T08:30:03Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11356-021-14974-4Environmental Science and Pollution Research.1614-74990944-1344http://hdl.handle.net/11449/22903310.1007/s11356-021-14974-42-s2.0-85108634229Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2022-04-29T08:30:03Zoai:repositorio.unesp.br:11449/229033Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:06:33.619449Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
title |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
spellingShingle |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna Assis, Rhayane Alves [UNESP] Agriculture Anurans Erythrocytic nuclear abnormalities Hepatic melanin Micronucleus Soybean cultivation |
title_short |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
title_full |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
title_fullStr |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
title_full_unstemmed |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
title_sort |
Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna |
author |
Assis, Rhayane Alves [UNESP] |
author_facet |
Assis, Rhayane Alves [UNESP] Rezende, Wadson Rodrigues [UNESP] dos Santos, Cirley Gomes Araújo Benvindo-Souza, Marcelino Amorim, Nathan Pereira Lima Borges, Rinneu Elias Franco-Belussi, Lilian [UNESP] De Oliveira, Classius [UNESP] de Souza Santos, Lia Raquel |
author_role |
author |
author2 |
Rezende, Wadson Rodrigues [UNESP] dos Santos, Cirley Gomes Araújo Benvindo-Souza, Marcelino Amorim, Nathan Pereira Lima Borges, Rinneu Elias Franco-Belussi, Lilian [UNESP] De Oliveira, Classius [UNESP] de Souza Santos, Lia Raquel |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Goiano Federal Institute Universidade Estadual Paulista (UNESP) Universidade Federal de Goiás (UFG) University of Rio Verde Federal University of Mato Grosso do Sul |
dc.contributor.author.fl_str_mv |
Assis, Rhayane Alves [UNESP] Rezende, Wadson Rodrigues [UNESP] dos Santos, Cirley Gomes Araújo Benvindo-Souza, Marcelino Amorim, Nathan Pereira Lima Borges, Rinneu Elias Franco-Belussi, Lilian [UNESP] De Oliveira, Classius [UNESP] de Souza Santos, Lia Raquel |
dc.subject.por.fl_str_mv |
Agriculture Anurans Erythrocytic nuclear abnormalities Hepatic melanin Micronucleus Soybean cultivation |
topic |
Agriculture Anurans Erythrocytic nuclear abnormalities Hepatic melanin Micronucleus Soybean cultivation |
description |
The sensitivity of anuran to the effects of habitat destruction and contamination has led to a preoccupying global decline in their populations. Morphological biomarkers such as micronuclei and other erythrocyte nuclear abnormalities (ENAs), as well as the occurrence of hepatic melanin, can be used to evaluate the effects of habitat impacts. In the present study, these two parameters were combined for the in situ assessment of the effects of soybean cultivation on the grassfrog, Leptodactylus fuscus. Specimens were also collected from a protected area to provide a reference site (non-agricultural environment). The frequency of some of the nuclear abnormalities in the animals from the soybean plantation was much higher than that recorded at the reference site, in particular micronuclei, which were 3.6 times more frequent in the plantation, lobulated nuclei (3.4 times more frequent), and reniform nuclei, which were four times more common than at the reference site. The combined analysis of all the ENAs together also revealed a frequency approximately 1.4 times higher in the animals from the soybean plantation, in comparison with the protected area. Smaller areas of hepatic melanin were observed in the specimens from the soybean plantation. These results provide further evidence of the sensitivity of anurans to habitat impacts and indicate that animals found in soybean plantations are susceptible to systematic alterations of their cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:30:03Z 2022-04-29T08:30:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-021-14974-4 Environmental Science and Pollution Research. 1614-7499 0944-1344 http://hdl.handle.net/11449/229033 10.1007/s11356-021-14974-4 2-s2.0-85108634229 |
url |
http://dx.doi.org/10.1007/s11356-021-14974-4 http://hdl.handle.net/11449/229033 |
identifier_str_mv |
Environmental Science and Pollution Research. 1614-7499 0944-1344 10.1007/s11356-021-14974-4 2-s2.0-85108634229 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128609605386240 |