Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0239171 http://hdl.handle.net/11449/208362 |
Resumo: | rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum. |
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Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantumrIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.Oswaldo Cruz Foundation Gonçalo Moniz Research Center Laboratory of Structural and MolecularPathology (LAPEM) Tissue Engineering and Immunopharmacology Laboratory (LETI) or Pathology andMolecular Biology Laboratory (LPBM)Department of Clinical Medicine Surgeryand Animal Reproduction São Paulo State University (UNESP) School of Veterinary MedicineDepartment of Clinical Medicine Surgeryand Animal Reproduction São Paulo State University (UNESP) School of Veterinary MedicineTissue Engineering and Immunopharmacology Laboratory (LETI) or Pathology andMolecular Biology Laboratory (LPBM)Universidade Estadual Paulista (Unesp)Santos, Catiule de OliveiraCosta, Sidnei Ferro [UNESP]Souza, Fabiana SantanaMendes, Jessica Mariane FerreiraPinheiro, Cristiane Garboggini Melo deMoreira, Diogo Rodrigo de MagalhãesSilva, Luciano KalabricLima, Valeria Marçal Felix de [UNESP]Oliveira, Geraldo Gileno de Sá2021-06-25T11:10:54Z2021-06-25T11:10:54Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0239171PLoS ONE, v. 16, n. 1 January, 2021.1932-6203http://hdl.handle.net/11449/20836210.1371/journal.pone.02391712-s2.0-85100230340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-09-04T18:03:58Zoai:repositorio.unesp.br:11449/208362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
title |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
spellingShingle |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum Santos, Catiule de Oliveira |
title_short |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
title_full |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
title_fullStr |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
title_full_unstemmed |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
title_sort |
Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum |
author |
Santos, Catiule de Oliveira |
author_facet |
Santos, Catiule de Oliveira Costa, Sidnei Ferro [UNESP] Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de [UNESP] Oliveira, Geraldo Gileno de Sá |
author_role |
author |
author2 |
Costa, Sidnei Ferro [UNESP] Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de [UNESP] Oliveira, Geraldo Gileno de Sá |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Tissue Engineering and Immunopharmacology Laboratory (LETI) or Pathology andMolecular Biology Laboratory (LPBM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Catiule de Oliveira Costa, Sidnei Ferro [UNESP] Souza, Fabiana Santana Mendes, Jessica Mariane Ferreira Pinheiro, Cristiane Garboggini Melo de Moreira, Diogo Rodrigo de Magalhães Silva, Luciano Kalabric Lima, Valeria Marçal Felix de [UNESP] Oliveira, Geraldo Gileno de Sá |
description |
rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:10:54Z 2021-06-25T11:10:54Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0239171 PLoS ONE, v. 16, n. 1 January, 2021. 1932-6203 http://hdl.handle.net/11449/208362 10.1371/journal.pone.0239171 2-s2.0-85100230340 |
url |
http://dx.doi.org/10.1371/journal.pone.0239171 http://hdl.handle.net/11449/208362 |
identifier_str_mv |
PLoS ONE, v. 16, n. 1 January, 2021. 1932-6203 10.1371/journal.pone.0239171 2-s2.0-85100230340 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021396173029376 |