Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots

Detalhes bibliográficos
Autor(a) principal: Birolli, Willian Garcia
Data de Publicação: 2020
Outros Autores: da Silva, Bianca Ferreira [UNESP], Rodrigues-Filho, Edson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.scitotenv.2020.140968
http://hdl.handle.net/11449/200840
Resumo: The pesticides belonging the strobilurin group are among the most common contaminants in the environment. In this work, biodegradation studies of the strobilurin fungicide Pyraclostrobin by bacteria from orange cultivation plots were performed aiming to contribute with the development of a bioremediation method. Experiments were performed in triplicate with validated methods, and optimization was performed by Central Composite Design and Response Surface Methodology. The strains were evaluated in liquid nutrient medium containing 100 mg L−1 of Pyraclostrobin, and decreased concentrations of 61.5 to 100.5 mg L−1 were determined after 5 days at 37 °C and 130 rpm, showing the importance of strain selection. When the five most efficient strains (Bacillus sp. CSA-13, Paenibacillus alvei CBMAI2221, Bacillus sp. CBMAI2222, Bacillus safensis CBMAI2220 and Bacillus aryabhattai CBMAI2223) were used in consortia, synergistic and antagonistic effects were observed accordingly to the employed combination of bacteria, resulting in 64.2 ± 3.9 to 95.4 ± 4.9 mg L−1 residual Pyraclostrobin. In addition, the formation of 1-(4-chlorophenyl)-1H-pyrazol-3-ol was quantified (0.59–0.01 mg L−1), and a new biodegradation pathway was proposed with 15 identified metabolites. Experiments were also performed in soil under controlled conditions (30 °C, 0–28 days, 100 mg kg−1 pesticide), and the native microbiome reduced the pesticide concentration to 70.4 ± 2.3 mg L−1, whereas the inoculation of an efficient bacterial consortium promoted clearly better results, 57.2 ± 3.9 mg L−1 residual Pyraclostrobin. This suggests that the introduction of these strains in soil in a bioaugmentation process increases decontamination. However, the native microbiome is important for a more efficient bioremediation.
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spelling Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots1-(4-Chlorophenyl)-1H-pyrazol-3-olBiodegradation pathwayBioremediationConsortiumNative microbiomePesticideThe pesticides belonging the strobilurin group are among the most common contaminants in the environment. In this work, biodegradation studies of the strobilurin fungicide Pyraclostrobin by bacteria from orange cultivation plots were performed aiming to contribute with the development of a bioremediation method. Experiments were performed in triplicate with validated methods, and optimization was performed by Central Composite Design and Response Surface Methodology. The strains were evaluated in liquid nutrient medium containing 100 mg L−1 of Pyraclostrobin, and decreased concentrations of 61.5 to 100.5 mg L−1 were determined after 5 days at 37 °C and 130 rpm, showing the importance of strain selection. When the five most efficient strains (Bacillus sp. CSA-13, Paenibacillus alvei CBMAI2221, Bacillus sp. CBMAI2222, Bacillus safensis CBMAI2220 and Bacillus aryabhattai CBMAI2223) were used in consortia, synergistic and antagonistic effects were observed accordingly to the employed combination of bacteria, resulting in 64.2 ± 3.9 to 95.4 ± 4.9 mg L−1 residual Pyraclostrobin. In addition, the formation of 1-(4-chlorophenyl)-1H-pyrazol-3-ol was quantified (0.59–0.01 mg L−1), and a new biodegradation pathway was proposed with 15 identified metabolites. Experiments were also performed in soil under controlled conditions (30 °C, 0–28 days, 100 mg kg−1 pesticide), and the native microbiome reduced the pesticide concentration to 70.4 ± 2.3 mg L−1, whereas the inoculation of an efficient bacterial consortium promoted clearly better results, 57.2 ± 3.9 mg L−1 residual Pyraclostrobin. This suggests that the introduction of these strains in soil in a bioaugmentation process increases decontamination. However, the native microbiome is important for a more efficient bioremediation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundo de Defesa da CitriculturaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Micromolecular Biochemistry of Microorganisms (LaBioMMi) Center for Exact Sciences and Technology Federal University of São Carlos, Via Washington Luiz, km 235, P.O. Box 676Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), P.O. Box 355Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), P.O. Box 355CAPES: 001FAPESP: 2008/10449-7FAPESP: 2016/09690-8FAPESP: 2017/19721-0FAPESP: 304867/2017-9Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Birolli, Willian Garciada Silva, Bianca Ferreira [UNESP]Rodrigues-Filho, Edson2020-12-12T02:17:30Z2020-12-12T02:17:30Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.scitotenv.2020.140968Science of the Total Environment, v. 746.1879-10260048-9697http://hdl.handle.net/11449/20084010.1016/j.scitotenv.2020.1409682-s2.0-85088918497Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScience of the Total Environmentinfo:eu-repo/semantics/openAccess2021-10-23T15:25:00Zoai:repositorio.unesp.br:11449/200840Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:43:55.194851Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
title Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
spellingShingle Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
Birolli, Willian Garcia
1-(4-Chlorophenyl)-1H-pyrazol-3-ol
Biodegradation pathway
Bioremediation
Consortium
Native microbiome
Pesticide
title_short Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
title_full Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
title_fullStr Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
title_full_unstemmed Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
title_sort Biodegradation of the fungicide Pyraclostrobin by bacteria from orange cultivation plots
author Birolli, Willian Garcia
author_facet Birolli, Willian Garcia
da Silva, Bianca Ferreira [UNESP]
Rodrigues-Filho, Edson
author_role author
author2 da Silva, Bianca Ferreira [UNESP]
Rodrigues-Filho, Edson
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Birolli, Willian Garcia
da Silva, Bianca Ferreira [UNESP]
Rodrigues-Filho, Edson
dc.subject.por.fl_str_mv 1-(4-Chlorophenyl)-1H-pyrazol-3-ol
Biodegradation pathway
Bioremediation
Consortium
Native microbiome
Pesticide
topic 1-(4-Chlorophenyl)-1H-pyrazol-3-ol
Biodegradation pathway
Bioremediation
Consortium
Native microbiome
Pesticide
description The pesticides belonging the strobilurin group are among the most common contaminants in the environment. In this work, biodegradation studies of the strobilurin fungicide Pyraclostrobin by bacteria from orange cultivation plots were performed aiming to contribute with the development of a bioremediation method. Experiments were performed in triplicate with validated methods, and optimization was performed by Central Composite Design and Response Surface Methodology. The strains were evaluated in liquid nutrient medium containing 100 mg L−1 of Pyraclostrobin, and decreased concentrations of 61.5 to 100.5 mg L−1 were determined after 5 days at 37 °C and 130 rpm, showing the importance of strain selection. When the five most efficient strains (Bacillus sp. CSA-13, Paenibacillus alvei CBMAI2221, Bacillus sp. CBMAI2222, Bacillus safensis CBMAI2220 and Bacillus aryabhattai CBMAI2223) were used in consortia, synergistic and antagonistic effects were observed accordingly to the employed combination of bacteria, resulting in 64.2 ± 3.9 to 95.4 ± 4.9 mg L−1 residual Pyraclostrobin. In addition, the formation of 1-(4-chlorophenyl)-1H-pyrazol-3-ol was quantified (0.59–0.01 mg L−1), and a new biodegradation pathway was proposed with 15 identified metabolites. Experiments were also performed in soil under controlled conditions (30 °C, 0–28 days, 100 mg kg−1 pesticide), and the native microbiome reduced the pesticide concentration to 70.4 ± 2.3 mg L−1, whereas the inoculation of an efficient bacterial consortium promoted clearly better results, 57.2 ± 3.9 mg L−1 residual Pyraclostrobin. This suggests that the introduction of these strains in soil in a bioaugmentation process increases decontamination. However, the native microbiome is important for a more efficient bioremediation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:17:30Z
2020-12-12T02:17:30Z
2020-12-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.scitotenv.2020.140968
Science of the Total Environment, v. 746.
1879-1026
0048-9697
http://hdl.handle.net/11449/200840
10.1016/j.scitotenv.2020.140968
2-s2.0-85088918497
url http://dx.doi.org/10.1016/j.scitotenv.2020.140968
http://hdl.handle.net/11449/200840
identifier_str_mv Science of the Total Environment, v. 746.
1879-1026
0048-9697
10.1016/j.scitotenv.2020.140968
2-s2.0-85088918497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Science of the Total Environment
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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