Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3732/apps.1300084 http://hdl.handle.net/11449/117525 |
Resumo: | Premise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies.Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana |
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Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)conservation geneticsGenipa americanamicrosatellite markerspopulation geneticsRubiaceaetree speciesPremise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies.Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americanaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, BrazilInst Florestal Sao Paulo, BR-01059970 Sao Paulo, BrazilUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, BrazilUniv Estadual Paulista, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, BrazilFAPESP: 10/19613-4CNPq: 473677/2010-5FAPESP: 11/01518-8Botanical Soc Amer IncUniversidade Estadual Paulista (Unesp)Inst Florestal Sao PauloUniversidade Estadual de Campinas (UNICAMP)Manoel, Ricardo de Oliveira [UNESP]Freitas, Miguel L. M.Barreto, Mariana A.Moraes, Mario Luis Teixeira de [UNESP]Souza, Anete P.Sebbenn, Alexandre M.2015-03-18T15:56:21Z2015-03-18T15:56:21Z2014-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3application/pdfhttp://dx.doi.org/10.3732/apps.1300084Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 3 p., 2014.2168-0450http://hdl.handle.net/11449/11752510.3732/apps.1300084WOS:000342559000005WOS000342559000005.pdf9803426672221802Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplications In Plant Sciences1.1870,746info:eu-repo/semantics/openAccess2024-07-05T19:08:04Zoai:repositorio.unesp.br:11449/117525Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:33.066962Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
title |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
spellingShingle |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) Manoel, Ricardo de Oliveira [UNESP] conservation genetics Genipa americana microsatellite markers population genetics Rubiaceae tree species |
title_short |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
title_full |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
title_fullStr |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
title_full_unstemmed |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
title_sort |
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae) |
author |
Manoel, Ricardo de Oliveira [UNESP] |
author_facet |
Manoel, Ricardo de Oliveira [UNESP] Freitas, Miguel L. M. Barreto, Mariana A. Moraes, Mario Luis Teixeira de [UNESP] Souza, Anete P. Sebbenn, Alexandre M. |
author_role |
author |
author2 |
Freitas, Miguel L. M. Barreto, Mariana A. Moraes, Mario Luis Teixeira de [UNESP] Souza, Anete P. Sebbenn, Alexandre M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Inst Florestal Sao Paulo Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Manoel, Ricardo de Oliveira [UNESP] Freitas, Miguel L. M. Barreto, Mariana A. Moraes, Mario Luis Teixeira de [UNESP] Souza, Anete P. Sebbenn, Alexandre M. |
dc.subject.por.fl_str_mv |
conservation genetics Genipa americana microsatellite markers population genetics Rubiaceae tree species |
topic |
conservation genetics Genipa americana microsatellite markers population genetics Rubiaceae tree species |
description |
Premise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies.Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-01 2015-03-18T15:56:21Z 2015-03-18T15:56:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3732/apps.1300084 Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 3 p., 2014. 2168-0450 http://hdl.handle.net/11449/117525 10.3732/apps.1300084 WOS:000342559000005 WOS000342559000005.pdf 9803426672221802 |
url |
http://dx.doi.org/10.3732/apps.1300084 http://hdl.handle.net/11449/117525 |
identifier_str_mv |
Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 3 p., 2014. 2168-0450 10.3732/apps.1300084 WOS:000342559000005 WOS000342559000005.pdf 9803426672221802 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applications In Plant Sciences 1.187 0,746 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3 application/pdf |
dc.publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128476807430144 |