High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.resp.2018.10.003 http://hdl.handle.net/11449/188214 |
Resumo: | Breathing disorders are commonly observed in association with obesity. Here we tested whether high-fat diet (HFD) impairs the chemoreflex ventilatory response. Male Holtzman rats (300–320 g) were fed with standard chow diet (SD) or HFD for 12 weeks. Then, tidal volume (V T ), respiratory frequency (f R ) and pulmonary ventilation (V E ) were determined in conscious rats during basal condition, hypercapnia (7% or 10% CO 2 ) or hypoxia (7% O 2 ). The mean arterial pressure (MAP), heart rate (HR) and baroreflex sensitivity were also evaluated in conscious rats. A group of anesthetized rats was used for the measurements of the activity of inspiratory (diaphragm) and expiratory (abdominal) muscles under the same gas conditions. Baseline f R , V T and V E were similar between SD and HFD rats. During hypercapnia, the increase of f R was exacerbated in conscious HFD rats (60 ± 3, vs. SD: 47 ± 3 Δ breaths.min −1 , P < 0.05). In anesthetized rats, hypercapnia strongly increased abdominal muscle activity in HFD group (238 ± 27, vs. basal condition: 100 ± 0.3%; P < 0.05), without significant change in SD group (129 ± 2.1, vs. basal condition: 100 ± 0.8%; P = 0.34). The ventilatory responses to hypoxia were similar between groups. In conscious HFD rats, MAP and HR were elevated and the baroreflex function was impaired (P < 0.05). These data demonstrated that 12 weeks of HFD exaggerate the ventilatory response activated by hypercapnia. The mechanisms involved in these responses need more investigation in future studies. |
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High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapniaBaroreflexBlood pressureChemoreflexDiaphragm motor activityHypoxiaSympathetic modulationBreathing disorders are commonly observed in association with obesity. Here we tested whether high-fat diet (HFD) impairs the chemoreflex ventilatory response. Male Holtzman rats (300–320 g) were fed with standard chow diet (SD) or HFD for 12 weeks. Then, tidal volume (V T ), respiratory frequency (f R ) and pulmonary ventilation (V E ) were determined in conscious rats during basal condition, hypercapnia (7% or 10% CO 2 ) or hypoxia (7% O 2 ). The mean arterial pressure (MAP), heart rate (HR) and baroreflex sensitivity were also evaluated in conscious rats. A group of anesthetized rats was used for the measurements of the activity of inspiratory (diaphragm) and expiratory (abdominal) muscles under the same gas conditions. Baseline f R , V T and V E were similar between SD and HFD rats. During hypercapnia, the increase of f R was exacerbated in conscious HFD rats (60 ± 3, vs. SD: 47 ± 3 Δ breaths.min −1 , P < 0.05). In anesthetized rats, hypercapnia strongly increased abdominal muscle activity in HFD group (238 ± 27, vs. basal condition: 100 ± 0.3%; P < 0.05), without significant change in SD group (129 ± 2.1, vs. basal condition: 100 ± 0.8%; P = 0.34). The ventilatory responses to hypoxia were similar between groups. In conscious HFD rats, MAP and HR were elevated and the baroreflex function was impaired (P < 0.05). These data demonstrated that 12 weeks of HFD exaggerate the ventilatory response activated by hypercapnia. The mechanisms involved in these responses need more investigation in future studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)Department of Clinical Analysis School of Pharmaceutical Sciences UNESPDepartment of Physiological Sciences Biological Sciences Center Federal University of Santa Catarina (UFSC), Rua Roberto Sampaio Gonzaga s/n, TrindadeDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)Department of Clinical Analysis School of Pharmaceutical Sciences UNESPFAPESP: 2013/13118-0FAPESP: 2013/14850-6FAPESP: 2013/17251-6CAPES: 20131972FAPESP: 2015/234677CNPq: 304873/2014-4CNPq: 310331/2017-0CNPq: 425586/2016-2Universidade Estadual Paulista (Unesp)Universidade Federal de Santa Catarina (UFSC)Speretta, Guilherme F. [UNESP]Lemes, Eduardo [UNESP]Vendramini, Regina C. [UNESP]Menani, José V. [UNESP]Zoccal, Daniel B. [UNESP]Colombari, Eduardo [UNESP]Colombari, Débora S.A. [UNESP]Bassi, Mirian [UNESP]2019-10-06T16:00:57Z2019-10-06T16:00:57Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article32-39http://dx.doi.org/10.1016/j.resp.2018.10.003Respiratory Physiology and Neurobiology, v. 258, p. 32-39.1878-15191569-9048http://hdl.handle.net/11449/18821410.1016/j.resp.2018.10.0032-s2.0-85054918493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRespiratory Physiology and Neurobiologyinfo:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/188214Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:25:24.912604Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
title |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
spellingShingle |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia Speretta, Guilherme F. [UNESP] Baroreflex Blood pressure Chemoreflex Diaphragm motor activity Hypoxia Sympathetic modulation |
title_short |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
title_full |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
title_fullStr |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
title_full_unstemmed |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
title_sort |
High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia |
author |
Speretta, Guilherme F. [UNESP] |
author_facet |
Speretta, Guilherme F. [UNESP] Lemes, Eduardo [UNESP] Vendramini, Regina C. [UNESP] Menani, José V. [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Eduardo [UNESP] Colombari, Débora S.A. [UNESP] Bassi, Mirian [UNESP] |
author_role |
author |
author2 |
Lemes, Eduardo [UNESP] Vendramini, Regina C. [UNESP] Menani, José V. [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Eduardo [UNESP] Colombari, Débora S.A. [UNESP] Bassi, Mirian [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
Speretta, Guilherme F. [UNESP] Lemes, Eduardo [UNESP] Vendramini, Regina C. [UNESP] Menani, José V. [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Eduardo [UNESP] Colombari, Débora S.A. [UNESP] Bassi, Mirian [UNESP] |
dc.subject.por.fl_str_mv |
Baroreflex Blood pressure Chemoreflex Diaphragm motor activity Hypoxia Sympathetic modulation |
topic |
Baroreflex Blood pressure Chemoreflex Diaphragm motor activity Hypoxia Sympathetic modulation |
description |
Breathing disorders are commonly observed in association with obesity. Here we tested whether high-fat diet (HFD) impairs the chemoreflex ventilatory response. Male Holtzman rats (300–320 g) were fed with standard chow diet (SD) or HFD for 12 weeks. Then, tidal volume (V T ), respiratory frequency (f R ) and pulmonary ventilation (V E ) were determined in conscious rats during basal condition, hypercapnia (7% or 10% CO 2 ) or hypoxia (7% O 2 ). The mean arterial pressure (MAP), heart rate (HR) and baroreflex sensitivity were also evaluated in conscious rats. A group of anesthetized rats was used for the measurements of the activity of inspiratory (diaphragm) and expiratory (abdominal) muscles under the same gas conditions. Baseline f R , V T and V E were similar between SD and HFD rats. During hypercapnia, the increase of f R was exacerbated in conscious HFD rats (60 ± 3, vs. SD: 47 ± 3 Δ breaths.min −1 , P < 0.05). In anesthetized rats, hypercapnia strongly increased abdominal muscle activity in HFD group (238 ± 27, vs. basal condition: 100 ± 0.3%; P < 0.05), without significant change in SD group (129 ± 2.1, vs. basal condition: 100 ± 0.8%; P = 0.34). The ventilatory responses to hypoxia were similar between groups. In conscious HFD rats, MAP and HR were elevated and the baroreflex function was impaired (P < 0.05). These data demonstrated that 12 weeks of HFD exaggerate the ventilatory response activated by hypercapnia. The mechanisms involved in these responses need more investigation in future studies. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-06T16:00:57Z 2019-10-06T16:00:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.resp.2018.10.003 Respiratory Physiology and Neurobiology, v. 258, p. 32-39. 1878-1519 1569-9048 http://hdl.handle.net/11449/188214 10.1016/j.resp.2018.10.003 2-s2.0-85054918493 |
url |
http://dx.doi.org/10.1016/j.resp.2018.10.003 http://hdl.handle.net/11449/188214 |
identifier_str_mv |
Respiratory Physiology and Neurobiology, v. 258, p. 32-39. 1878-1519 1569-9048 10.1016/j.resp.2018.10.003 2-s2.0-85054918493 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Respiratory Physiology and Neurobiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
32-39 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128511765905408 |