Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11033-021-06640-2 http://hdl.handle.net/11449/229358 |
Resumo: | Background: Obesity is considered a chronic inflammatory disease and transforming growth factor beta 1 (TGFβ1) might exert important roles in disease pathogenesis regulating adipocyte differentiation and immune-inflammatory environment. However, the role of this cytokine as a biomarker in obesity is poorly addressed. Therefore, the present study aimed to evaluate the impact of TGFB1 polymorphisms and TGFβ1 plasmatic levels in obesity Methods and results: TGFB1 promoter region polymorphisms (rs1800468, G-800A and rs1800469, C-509 T) were evaluated in 75 obese patients and 45 eutrophic patients through PCR–RFLP and plasmatic TGFβ1 was quantified through ELISA from 37 of the obese patients, and correlations with clinical and biochemical parameters were tested. Despite no association was found between TGFB1 polymorphisms and obesity susceptibility, several correlations with clinical data were noted. Among others, AC haplotype negatively correlated with plasmatic TGFβ1, while plasmatic TGFβ1 negatively correlated with C-reactive protein and positively correlated with liver abnormalities on ultrasound and, specifically, with steatosis presence and degree. Conversely, GT haplotype, which associates with higher TGFβ1 production, was also positively correlated with the same parameters of liver abnormalities. Further, plasmatic vitamin D negatively correlated with TGFβ1, while positively correlated with AC haplotype. Conclusion: Overall, the results indicate that TGFβ1 might exert important roles in obesity pathophysiology and correlate with biochemical and clinical parameters both at systemic protein as well as at genetic level. Importantly, the consistent positive correlation at both levels with steatosis might suggest this cytokine as a biomarker for this hepatic abnormality in obese patients. |
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Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis developmentInflammationObesityOverweightSteatosisTransforming growth factor betaBackground: Obesity is considered a chronic inflammatory disease and transforming growth factor beta 1 (TGFβ1) might exert important roles in disease pathogenesis regulating adipocyte differentiation and immune-inflammatory environment. However, the role of this cytokine as a biomarker in obesity is poorly addressed. Therefore, the present study aimed to evaluate the impact of TGFB1 polymorphisms and TGFβ1 plasmatic levels in obesity Methods and results: TGFB1 promoter region polymorphisms (rs1800468, G-800A and rs1800469, C-509 T) were evaluated in 75 obese patients and 45 eutrophic patients through PCR–RFLP and plasmatic TGFβ1 was quantified through ELISA from 37 of the obese patients, and correlations with clinical and biochemical parameters were tested. Despite no association was found between TGFB1 polymorphisms and obesity susceptibility, several correlations with clinical data were noted. Among others, AC haplotype negatively correlated with plasmatic TGFβ1, while plasmatic TGFβ1 negatively correlated with C-reactive protein and positively correlated with liver abnormalities on ultrasound and, specifically, with steatosis presence and degree. Conversely, GT haplotype, which associates with higher TGFβ1 production, was also positively correlated with the same parameters of liver abnormalities. Further, plasmatic vitamin D negatively correlated with TGFβ1, while positively correlated with AC haplotype. Conclusion: Overall, the results indicate that TGFβ1 might exert important roles in obesity pathophysiology and correlate with biochemical and clinical parameters both at systemic protein as well as at genetic level. Importantly, the consistent positive correlation at both levels with steatosis might suggest this cytokine as a biomarker for this hepatic abnormality in obese patients.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of General Biology Biological Sciences Center Londrina State University (UEL)Department of Pathological Sciences Biological Sciences Center Londrina State University (UEL)Clinical Surgery Department Federal University of Parana (UFPR)School of Medicine Department of Pathology São Paulo State University (UNESP)Laboratory of DNA Polymorphisms and Immunology Department of Pathological Sciences Biological Sciences Center State University of Londrina, PR445, Km 380 Celso Garcia Cid highwaySchool of Medicine Department of Pathology São Paulo State University (UNESP)CNPq: 152170/2019-7CNPq: 306386/2017-8Universidade Estadual de Londrina (UEL)Universidade Federal do Paraná (UFPR)Universidade Estadual Paulista (UNESP)Felicidade, Ingrid [UNESP]Bocchi, MayaraRamos, Marília Rizzon ZaparolliCarlos, Ligia de OliveiraWagner, Nathalia Ramori FarinhaCampos, Antônio Carlos LigockiRibeiro, Lúcia Regina [UNESP]Mantovani, Mário SérgioWatanabe, Maria Angelica EharaVitiello, Glauco Akelinghton Freire2022-04-29T08:32:07Z2022-04-29T08:32:07Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6401-6411http://dx.doi.org/10.1007/s11033-021-06640-2Molecular Biology Reports, v. 48, n. 9, p. 6401-6411, 2021.1573-49780301-4851http://hdl.handle.net/11449/22935810.1007/s11033-021-06640-22-s2.0-85112774014Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Biology Reportsinfo:eu-repo/semantics/openAccess2024-09-03T13:14:30Zoai:repositorio.unesp.br:11449/229358Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
title |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
spellingShingle |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development Felicidade, Ingrid [UNESP] Inflammation Obesity Overweight Steatosis Transforming growth factor beta |
title_short |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
title_full |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
title_fullStr |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
title_full_unstemmed |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
title_sort |
Transforming growth factor beta 1 (TGFβ1) plasmatic levels and haplotype structures in obesity: a role for TGFβ1 in steatosis development |
author |
Felicidade, Ingrid [UNESP] |
author_facet |
Felicidade, Ingrid [UNESP] Bocchi, Mayara Ramos, Marília Rizzon Zaparolli Carlos, Ligia de Oliveira Wagner, Nathalia Ramori Farinha Campos, Antônio Carlos Ligocki Ribeiro, Lúcia Regina [UNESP] Mantovani, Mário Sérgio Watanabe, Maria Angelica Ehara Vitiello, Glauco Akelinghton Freire |
author_role |
author |
author2 |
Bocchi, Mayara Ramos, Marília Rizzon Zaparolli Carlos, Ligia de Oliveira Wagner, Nathalia Ramori Farinha Campos, Antônio Carlos Ligocki Ribeiro, Lúcia Regina [UNESP] Mantovani, Mário Sérgio Watanabe, Maria Angelica Ehara Vitiello, Glauco Akelinghton Freire |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Felicidade, Ingrid [UNESP] Bocchi, Mayara Ramos, Marília Rizzon Zaparolli Carlos, Ligia de Oliveira Wagner, Nathalia Ramori Farinha Campos, Antônio Carlos Ligocki Ribeiro, Lúcia Regina [UNESP] Mantovani, Mário Sérgio Watanabe, Maria Angelica Ehara Vitiello, Glauco Akelinghton Freire |
dc.subject.por.fl_str_mv |
Inflammation Obesity Overweight Steatosis Transforming growth factor beta |
topic |
Inflammation Obesity Overweight Steatosis Transforming growth factor beta |
description |
Background: Obesity is considered a chronic inflammatory disease and transforming growth factor beta 1 (TGFβ1) might exert important roles in disease pathogenesis regulating adipocyte differentiation and immune-inflammatory environment. However, the role of this cytokine as a biomarker in obesity is poorly addressed. Therefore, the present study aimed to evaluate the impact of TGFB1 polymorphisms and TGFβ1 plasmatic levels in obesity Methods and results: TGFB1 promoter region polymorphisms (rs1800468, G-800A and rs1800469, C-509 T) were evaluated in 75 obese patients and 45 eutrophic patients through PCR–RFLP and plasmatic TGFβ1 was quantified through ELISA from 37 of the obese patients, and correlations with clinical and biochemical parameters were tested. Despite no association was found between TGFB1 polymorphisms and obesity susceptibility, several correlations with clinical data were noted. Among others, AC haplotype negatively correlated with plasmatic TGFβ1, while plasmatic TGFβ1 negatively correlated with C-reactive protein and positively correlated with liver abnormalities on ultrasound and, specifically, with steatosis presence and degree. Conversely, GT haplotype, which associates with higher TGFβ1 production, was also positively correlated with the same parameters of liver abnormalities. Further, plasmatic vitamin D negatively correlated with TGFβ1, while positively correlated with AC haplotype. Conclusion: Overall, the results indicate that TGFβ1 might exert important roles in obesity pathophysiology and correlate with biochemical and clinical parameters both at systemic protein as well as at genetic level. Importantly, the consistent positive correlation at both levels with steatosis might suggest this cytokine as a biomarker for this hepatic abnormality in obese patients. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-01 2022-04-29T08:32:07Z 2022-04-29T08:32:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11033-021-06640-2 Molecular Biology Reports, v. 48, n. 9, p. 6401-6411, 2021. 1573-4978 0301-4851 http://hdl.handle.net/11449/229358 10.1007/s11033-021-06640-2 2-s2.0-85112774014 |
url |
http://dx.doi.org/10.1007/s11033-021-06640-2 http://hdl.handle.net/11449/229358 |
identifier_str_mv |
Molecular Biology Reports, v. 48, n. 9, p. 6401-6411, 2021. 1573-4978 0301-4851 10.1007/s11033-021-06640-2 2-s2.0-85112774014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Molecular Biology Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6401-6411 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021360937730048 |