Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells

Detalhes bibliográficos
Autor(a) principal: Constantino, Flávia Bessi [UNESP]
Data de Publicação: 2021
Outros Autores: Cury, Sarah Santiloni [UNESP], Nogueira, Celia Regina [UNESP], Carvalho, Robson Francisco [UNESP], Justulin, Luis Antonio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmolb.2021.614728
http://hdl.handle.net/11449/233826
Resumo: The SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell entry mediators are expressed at low levels in placental cells, the receptors for viruses that cause congenital infections such as the cytomegalovirus and Zika virus are highly expressed in these cells. Here we analyzed the transcriptional profile (microarray and single-cell RNA-Seq) of proteins potentially interacting with coronaviruses to identify non- canonical mediators of SARS-CoV-2 infection and replication in the placenta. Despite low levels of the canonical cell entry mediators ACE2 and TMPRSS2, we show that cells of the syncytiotrophoblast, villous cytotrophoblast, and extravillous trophoblast co-express high levels of the potential non-canonical cell-entry mediators DPP4 and CTSL. We also found changes in the expression of DAAM1 and PAICS genes during pregnancy, which are translated into proteins also predicted to interact with coronaviruses proteins. These results provide new insight into the interaction between SARS-CoV-2 and host proteins that may act as non-canonical routes for SARS-CoV-2 infection and replication in the placenta cells.
id UNSP_d4b9308d66562c0103e36e689c269944
oai_identifier_str oai:repositorio.unesp.br:11449/233826
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta CellsCOVID-19CTSLDAAM1DPP4PAICSplacentaSARS-CoV-2virus entry mediatorThe SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell entry mediators are expressed at low levels in placental cells, the receptors for viruses that cause congenital infections such as the cytomegalovirus and Zika virus are highly expressed in these cells. Here we analyzed the transcriptional profile (microarray and single-cell RNA-Seq) of proteins potentially interacting with coronaviruses to identify non- canonical mediators of SARS-CoV-2 infection and replication in the placenta. Despite low levels of the canonical cell entry mediators ACE2 and TMPRSS2, we show that cells of the syncytiotrophoblast, villous cytotrophoblast, and extravillous trophoblast co-express high levels of the potential non-canonical cell-entry mediators DPP4 and CTSL. We also found changes in the expression of DAAM1 and PAICS genes during pregnancy, which are translated into proteins also predicted to interact with coronaviruses proteins. These results provide new insight into the interaction between SARS-CoV-2 and host proteins that may act as non-canonical routes for SARS-CoV-2 infection and replication in the placenta cells.Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Internal Clinic Botucatu Medicine School São Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Internal Clinic Botucatu Medicine School São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Constantino, Flávia Bessi [UNESP]Cury, Sarah Santiloni [UNESP]Nogueira, Celia Regina [UNESP]Carvalho, Robson Francisco [UNESP]Justulin, Luis Antonio [UNESP]2022-05-01T10:35:04Z2022-05-01T10:35:04Z2021-11-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmolb.2021.614728Frontiers in Molecular Biosciences, v. 8.2296-889Xhttp://hdl.handle.net/11449/23382610.3389/fmolb.2021.6147282-s2.0-85119595602Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Molecular Biosciencesinfo:eu-repo/semantics/openAccess2024-08-14T17:22:59Zoai:repositorio.unesp.br:11449/233826Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
spellingShingle Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
Constantino, Flávia Bessi [UNESP]
COVID-19
CTSL
DAAM1
DPP4
PAICS
placenta
SARS-CoV-2
virus entry mediator
title_short Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_full Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_fullStr Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_full_unstemmed Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
title_sort Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells
author Constantino, Flávia Bessi [UNESP]
author_facet Constantino, Flávia Bessi [UNESP]
Cury, Sarah Santiloni [UNESP]
Nogueira, Celia Regina [UNESP]
Carvalho, Robson Francisco [UNESP]
Justulin, Luis Antonio [UNESP]
author_role author
author2 Cury, Sarah Santiloni [UNESP]
Nogueira, Celia Regina [UNESP]
Carvalho, Robson Francisco [UNESP]
Justulin, Luis Antonio [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Constantino, Flávia Bessi [UNESP]
Cury, Sarah Santiloni [UNESP]
Nogueira, Celia Regina [UNESP]
Carvalho, Robson Francisco [UNESP]
Justulin, Luis Antonio [UNESP]
dc.subject.por.fl_str_mv COVID-19
CTSL
DAAM1
DPP4
PAICS
placenta
SARS-CoV-2
virus entry mediator
topic COVID-19
CTSL
DAAM1
DPP4
PAICS
placenta
SARS-CoV-2
virus entry mediator
description The SARS-CoV-2 is the causative agent of the COVID-19 pandemic. The data available about COVID-19 during pregnancy have demonstrated placental infection; however, the mechanisms associated with intrauterine transmission of SARS-CoV-2 is still debated. Intriguingly, while canonical SARS-CoV-2 cell entry mediators are expressed at low levels in placental cells, the receptors for viruses that cause congenital infections such as the cytomegalovirus and Zika virus are highly expressed in these cells. Here we analyzed the transcriptional profile (microarray and single-cell RNA-Seq) of proteins potentially interacting with coronaviruses to identify non- canonical mediators of SARS-CoV-2 infection and replication in the placenta. Despite low levels of the canonical cell entry mediators ACE2 and TMPRSS2, we show that cells of the syncytiotrophoblast, villous cytotrophoblast, and extravillous trophoblast co-express high levels of the potential non-canonical cell-entry mediators DPP4 and CTSL. We also found changes in the expression of DAAM1 and PAICS genes during pregnancy, which are translated into proteins also predicted to interact with coronaviruses proteins. These results provide new insight into the interaction between SARS-CoV-2 and host proteins that may act as non-canonical routes for SARS-CoV-2 infection and replication in the placenta cells.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-08
2022-05-01T10:35:04Z
2022-05-01T10:35:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmolb.2021.614728
Frontiers in Molecular Biosciences, v. 8.
2296-889X
http://hdl.handle.net/11449/233826
10.3389/fmolb.2021.614728
2-s2.0-85119595602
url http://dx.doi.org/10.3389/fmolb.2021.614728
http://hdl.handle.net/11449/233826
identifier_str_mv Frontiers in Molecular Biosciences, v. 8.
2296-889X
10.3389/fmolb.2021.614728
2-s2.0-85119595602
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Molecular Biosciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128143273230336