Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascihealthsci.v37i1.24348 http://hdl.handle.net/11449/232421 |
Resumo: | This study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-filled canals. Culture methods and 16S rDNA assay were used to identify E. faecalis. The antimicrobial susceptibility of E. faecalis was determined by MIC values using the E test. Cultivable strains of E. faecalis were investigated for virulence factors by PCR technique. E. faecalis were detected by culture (7/30), traditional PCR assay (13/30) and nested PCR (23/30). Both PCR were significantly more effective than culture in detecting E. faecalis (p < 0.05). All tested E. faecalis were highly sensitive to amoxicillin. Some strains of E. faecalis were resistant to antibiotics such as rifampicin (4/12), erythromycin (3/12) and azythromycin (8/12). The genes efaA and ace were detected in all isolates. The other virulence genes were found in 91.6 (gelE), 83.3 (asa), 25 (esp) and 16.6% (cylA). Strains of E. faecalis isolated from root-filled canals showed virulence factors related to adherence. They also showed resistance to some antibiotics commonly used in dentistry. |
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Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methodsInvestigação in vivo de Enterococcus faecalis em retratamento endodôntico por meio de métodos fenotípicos e genotípicosAntibioticsAntimicrobial susceptibilityEndodonticsVirulenceThis study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-filled canals. Culture methods and 16S rDNA assay were used to identify E. faecalis. The antimicrobial susceptibility of E. faecalis was determined by MIC values using the E test. Cultivable strains of E. faecalis were investigated for virulence factors by PCR technique. E. faecalis were detected by culture (7/30), traditional PCR assay (13/30) and nested PCR (23/30). Both PCR were significantly more effective than culture in detecting E. faecalis (p < 0.05). All tested E. faecalis were highly sensitive to amoxicillin. Some strains of E. faecalis were resistant to antibiotics such as rifampicin (4/12), erythromycin (3/12) and azythromycin (8/12). The genes efaA and ace were detected in all isolates. The other virulence genes were found in 91.6 (gelE), 83.3 (asa), 25 (esp) and 16.6% (cylA). Strains of E. faecalis isolated from root-filled canals showed virulence factors related to adherence. They also showed resistance to some antibiotics commonly used in dentistry.Departamento de Odontologia Restauradora Divisão de Endodontia Universidade Estadual de Campinas, Av. Limeira, 901Departamento de Odontologia Universidade Estadual de MaringáDepartamento de Odontologia Restauradora Divisão de Endodontia Universidade Estadual de São PauloDepartamento de Odontologia Restauradora Divisão de Endodontia Universidade Estadual de São PauloUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual de Maringá (UEM)Universidade Estadual Paulista (UNESP)Endo, Marcos SergioSignoretti, Fernanda Graziela CorrêaKitayama, Vivian SayuriMarinho, Ariane Cássia SalustianoMartinho, Frederico Canato [UNESP]Gomes, Brenda Paula F. De Almeida2022-04-29T15:50:17Z2022-04-29T15:50:17Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-103http://dx.doi.org/10.4025/actascihealthsci.v37i1.24348Acta Scientiarum - Health Sciences, v. 37, n. 1, p. 95-103, 2015.1807-86481679-9291http://hdl.handle.net/11449/23242110.4025/actascihealthsci.v37i1.243482-s2.0-84932650655Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum - Health Sciencesinfo:eu-repo/semantics/openAccess2022-04-29T15:50:17Zoai:repositorio.unesp.br:11449/232421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:16.699048Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods Investigação in vivo de Enterococcus faecalis em retratamento endodôntico por meio de métodos fenotípicos e genotípicos |
title |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
spellingShingle |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods Endo, Marcos Sergio Antibiotics Antimicrobial susceptibility Endodontics Virulence |
title_short |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
title_full |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
title_fullStr |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
title_full_unstemmed |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
title_sort |
Investigation in vivo of enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods |
author |
Endo, Marcos Sergio |
author_facet |
Endo, Marcos Sergio Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato [UNESP] Gomes, Brenda Paula F. De Almeida |
author_role |
author |
author2 |
Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato [UNESP] Gomes, Brenda Paula F. De Almeida |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Endo, Marcos Sergio Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato [UNESP] Gomes, Brenda Paula F. De Almeida |
dc.subject.por.fl_str_mv |
Antibiotics Antimicrobial susceptibility Endodontics Virulence |
topic |
Antibiotics Antimicrobial susceptibility Endodontics Virulence |
description |
This study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-filled canals. Culture methods and 16S rDNA assay were used to identify E. faecalis. The antimicrobial susceptibility of E. faecalis was determined by MIC values using the E test. Cultivable strains of E. faecalis were investigated for virulence factors by PCR technique. E. faecalis were detected by culture (7/30), traditional PCR assay (13/30) and nested PCR (23/30). Both PCR were significantly more effective than culture in detecting E. faecalis (p < 0.05). All tested E. faecalis were highly sensitive to amoxicillin. Some strains of E. faecalis were resistant to antibiotics such as rifampicin (4/12), erythromycin (3/12) and azythromycin (8/12). The genes efaA and ace were detected in all isolates. The other virulence genes were found in 91.6 (gelE), 83.3 (asa), 25 (esp) and 16.6% (cylA). Strains of E. faecalis isolated from root-filled canals showed virulence factors related to adherence. They also showed resistance to some antibiotics commonly used in dentistry. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2022-04-29T15:50:17Z 2022-04-29T15:50:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascihealthsci.v37i1.24348 Acta Scientiarum - Health Sciences, v. 37, n. 1, p. 95-103, 2015. 1807-8648 1679-9291 http://hdl.handle.net/11449/232421 10.4025/actascihealthsci.v37i1.24348 2-s2.0-84932650655 |
url |
http://dx.doi.org/10.4025/actascihealthsci.v37i1.24348 http://hdl.handle.net/11449/232421 |
identifier_str_mv |
Acta Scientiarum - Health Sciences, v. 37, n. 1, p. 95-103, 2015. 1807-8648 1679-9291 10.4025/actascihealthsci.v37i1.24348 2-s2.0-84932650655 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum - Health Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
95-103 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129583836299264 |