In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/cbdv.202100948 http://hdl.handle.net/11449/230184 |
Resumo: | Schistosomiasis, a tropical disease caused by flatworms, may affect the liver, spleen, bladder, and intestine. Casearia sylvestris Swartz, a medicinal plant, displays antiprotozoal, antimicrobial, antifungal, and antiulcer activities. We have evaluated the in vitro schistosomicidal activity of two C. sylvestris varieties against Schistosoma mansoni adult worms at concentrations between 12.5 and 200 μg/mL. At 100 and 200 μg/mL, the ethanolic C. sylvestris var. sylvestris leaf extract enriched in casearin-like diterpenes eliminated 100 % of the parasites after incubation for 72 h and 48 h, respectively, whilst the same extract at 200 μg/mL eliminated 96 %, 100 %, and 100 % of the parasites after incubation for 24, 48, and 72 h, respectively. On the other hand, the hydroalcoholic C. sylvestris var. lingua leaf extract at 200 μg/mL eliminated 60.4 and 66.7 % of the parasites after incubation for 48 and 72 h, respectively. The presence of casearin-like diterpenes and glycosylated flavonoids was confirmed based on chromatographic techniques and mass spectrometry data. |
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In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris VarietiesCasearia sylvestriscasearinflavonoidsneglected diseasesSchistosoma mansoniSchistosomiasis, a tropical disease caused by flatworms, may affect the liver, spleen, bladder, and intestine. Casearia sylvestris Swartz, a medicinal plant, displays antiprotozoal, antimicrobial, antifungal, and antiulcer activities. We have evaluated the in vitro schistosomicidal activity of two C. sylvestris varieties against Schistosoma mansoni adult worms at concentrations between 12.5 and 200 μg/mL. At 100 and 200 μg/mL, the ethanolic C. sylvestris var. sylvestris leaf extract enriched in casearin-like diterpenes eliminated 100 % of the parasites after incubation for 72 h and 48 h, respectively, whilst the same extract at 200 μg/mL eliminated 96 %, 100 %, and 100 % of the parasites after incubation for 24, 48, and 72 h, respectively. On the other hand, the hydroalcoholic C. sylvestris var. lingua leaf extract at 200 μg/mL eliminated 60.4 and 66.7 % of the parasites after incubation for 48 and 72 h, respectively. The presence of casearin-like diterpenes and glycosylated flavonoids was confirmed based on chromatographic techniques and mass spectrometry data.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Drugs and Medicines São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara-Jaú Road, Km 01, s/n, 14.801-903, SPResearch Group on Natural Products Center for Research in Sciences and Technology University of Franca, São PauloDepartment of Chemistry University of São Paulo (USP) Faculty of Philosophy, Sciences and Letters, Av. do Café, s/n, 14.040-903, SPDepartment of Drugs and Medicines São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara-Jaú Road, Km 01, s/n, 14.801-903, SPUniversidade Estadual Paulista (UNESP)University of FrancaUniversidade de São Paulo (USP)Carvalho, Flávio A. [UNESP]da Silva, Breno C.Oda, Fernando B. [UNESP]Junior, Júlio C. P. [UNESP]Crevelin, Eduardo J.Crotti, Antônio E. M.Caldas, Lizandra G. M.dos Santos, André G. [UNESP]2022-04-29T08:38:18Z2022-04-29T08:38:18Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/cbdv.202100948Chemistry and Biodiversity, v. 19, n. 2, 2022.1612-18801612-1872http://hdl.handle.net/11449/23018410.1002/cbdv.2021009482-s2.0-85122443189Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemistry and Biodiversityinfo:eu-repo/semantics/openAccess2024-06-24T13:45:28Zoai:repositorio.unesp.br:11449/230184Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:57:30.585205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
title |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
spellingShingle |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties Carvalho, Flávio A. [UNESP] Casearia sylvestris casearin flavonoids neglected diseases Schistosoma mansoni |
title_short |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
title_full |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
title_fullStr |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
title_full_unstemmed |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
title_sort |
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties |
author |
Carvalho, Flávio A. [UNESP] |
author_facet |
Carvalho, Flávio A. [UNESP] da Silva, Breno C. Oda, Fernando B. [UNESP] Junior, Júlio C. P. [UNESP] Crevelin, Eduardo J. Crotti, Antônio E. M. Caldas, Lizandra G. M. dos Santos, André G. [UNESP] |
author_role |
author |
author2 |
da Silva, Breno C. Oda, Fernando B. [UNESP] Junior, Júlio C. P. [UNESP] Crevelin, Eduardo J. Crotti, Antônio E. M. Caldas, Lizandra G. M. dos Santos, André G. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Franca Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Carvalho, Flávio A. [UNESP] da Silva, Breno C. Oda, Fernando B. [UNESP] Junior, Júlio C. P. [UNESP] Crevelin, Eduardo J. Crotti, Antônio E. M. Caldas, Lizandra G. M. dos Santos, André G. [UNESP] |
dc.subject.por.fl_str_mv |
Casearia sylvestris casearin flavonoids neglected diseases Schistosoma mansoni |
topic |
Casearia sylvestris casearin flavonoids neglected diseases Schistosoma mansoni |
description |
Schistosomiasis, a tropical disease caused by flatworms, may affect the liver, spleen, bladder, and intestine. Casearia sylvestris Swartz, a medicinal plant, displays antiprotozoal, antimicrobial, antifungal, and antiulcer activities. We have evaluated the in vitro schistosomicidal activity of two C. sylvestris varieties against Schistosoma mansoni adult worms at concentrations between 12.5 and 200 μg/mL. At 100 and 200 μg/mL, the ethanolic C. sylvestris var. sylvestris leaf extract enriched in casearin-like diterpenes eliminated 100 % of the parasites after incubation for 72 h and 48 h, respectively, whilst the same extract at 200 μg/mL eliminated 96 %, 100 %, and 100 % of the parasites after incubation for 24, 48, and 72 h, respectively. On the other hand, the hydroalcoholic C. sylvestris var. lingua leaf extract at 200 μg/mL eliminated 60.4 and 66.7 % of the parasites after incubation for 48 and 72 h, respectively. The presence of casearin-like diterpenes and glycosylated flavonoids was confirmed based on chromatographic techniques and mass spectrometry data. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:18Z 2022-04-29T08:38:18Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/cbdv.202100948 Chemistry and Biodiversity, v. 19, n. 2, 2022. 1612-1880 1612-1872 http://hdl.handle.net/11449/230184 10.1002/cbdv.202100948 2-s2.0-85122443189 |
url |
http://dx.doi.org/10.1002/cbdv.202100948 http://hdl.handle.net/11449/230184 |
identifier_str_mv |
Chemistry and Biodiversity, v. 19, n. 2, 2022. 1612-1880 1612-1872 10.1002/cbdv.202100948 2-s2.0-85122443189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemistry and Biodiversity |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128586698194944 |