A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis

Detalhes bibliográficos
Autor(a) principal: Hudari, Felipe Fantinato [UNESP]
Data de Publicação: 2017
Outros Autores: Zanoni, Maria Valnice Boldrin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00604-017-2443-5
http://hdl.handle.net/11449/179137
Resumo: The dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample.
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spelling A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysisBumetanideDiureticsDopingStripping voltammetryUrine sampleVoltammetric sensorThe dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample.Universidade Estadual Paulista (Unesp) Instituto de Química Araraquara, Av. Prof. Francisco Degni, 55Universidade Estadual Paulista (Unesp) Instituto de Química Araraquara, Av. Prof. Francisco Degni, 55Universidade Estadual Paulista (Unesp)Hudari, Felipe Fantinato [UNESP]Zanoni, Maria Valnice Boldrin [UNESP]2018-12-11T17:33:54Z2018-12-11T17:33:54Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4117-4124application/pdfhttp://dx.doi.org/10.1007/s00604-017-2443-5Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017.1436-50730026-3672http://hdl.handle.net/11449/17913710.1007/s00604-017-2443-52-s2.0-850285418132-s2.0-85028541813.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochimica Acta1,2401,240info:eu-repo/semantics/openAccess2023-10-28T06:08:38Zoai:repositorio.unesp.br:11449/179137Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:15:39.526366Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
title A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
spellingShingle A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
Hudari, Felipe Fantinato [UNESP]
Bumetanide
Diuretics
Doping
Stripping voltammetry
Urine sample
Voltammetric sensor
title_short A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
title_full A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
title_fullStr A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
title_full_unstemmed A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
title_sort A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
author Hudari, Felipe Fantinato [UNESP]
author_facet Hudari, Felipe Fantinato [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
author_role author
author2 Zanoni, Maria Valnice Boldrin [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Hudari, Felipe Fantinato [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
dc.subject.por.fl_str_mv Bumetanide
Diuretics
Doping
Stripping voltammetry
Urine sample
Voltammetric sensor
topic Bumetanide
Diuretics
Doping
Stripping voltammetry
Urine sample
Voltammetric sensor
description The dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
2018-12-11T17:33:54Z
2018-12-11T17:33:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00604-017-2443-5
Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017.
1436-5073
0026-3672
http://hdl.handle.net/11449/179137
10.1007/s00604-017-2443-5
2-s2.0-85028541813
2-s2.0-85028541813.pdf
url http://dx.doi.org/10.1007/s00604-017-2443-5
http://hdl.handle.net/11449/179137
identifier_str_mv Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017.
1436-5073
0026-3672
10.1007/s00604-017-2443-5
2-s2.0-85028541813
2-s2.0-85028541813.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microchimica Acta
1,240
1,240
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4117-4124
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128625750310912

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