Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jbm.b.35141 http://hdl.handle.net/11449/241500 |
Resumo: | The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol–gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro–Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal–Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering. |
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Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applicationsbioglassbiopolymersbone regenerationcobaltpolylactic acidstrontiumtissue engineeringThe development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol–gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro–Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal–Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.Department of Dental Materials and Prosthodontics Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloTechnological Institute of Aeronautics (ITA) Praça Marechal Eduardo Gomes, São PauloDepartment of Social Dentistry and Children's Clinic Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloDepartment of Dental Materials and Prosthodontics Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloDepartment of Social Dentistry and Children's Clinic Institute of Science and Technology of São José dos Campos São Paulo State University (UNESP), São PauloUniversidade Estadual Paulista (UNESP)Praça Marechal Eduardo Gomesde Souza, Joyce Rodrigues [UNESP]Kukulka, Elisa Camargo [UNESP]Araújo, Juliani Caroline Ribeiro [UNESP]Campos, Tiago Moreira Bastosdo Prado, Renata Falchete [UNESP]de Vasconcellos, Luana Marotta Reis [UNESP]Thin, Gilmar PatrocínioBorges, Alexandre Luiz Souto [UNESP]2023-03-01T21:06:43Z2023-03-01T21:06:43Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jbm.b.35141Journal of Biomedical Materials Research - Part B Applied Biomaterials.1552-49811552-4973http://hdl.handle.net/11449/24150010.1002/jbm.b.351412-s2.0-85135804651Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biomedical Materials Research - Part B Applied Biomaterialsinfo:eu-repo/semantics/openAccess2023-03-01T21:06:44Zoai:repositorio.unesp.br:11449/241500Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:25:58.990186Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
title |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
spellingShingle |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications de Souza, Joyce Rodrigues [UNESP] bioglass biopolymers bone regeneration cobalt polylactic acid strontium tissue engineering |
title_short |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
title_full |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
title_fullStr |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
title_full_unstemmed |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
title_sort |
Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications |
author |
de Souza, Joyce Rodrigues [UNESP] |
author_facet |
de Souza, Joyce Rodrigues [UNESP] Kukulka, Elisa Camargo [UNESP] Araújo, Juliani Caroline Ribeiro [UNESP] Campos, Tiago Moreira Bastos do Prado, Renata Falchete [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Thin, Gilmar Patrocínio Borges, Alexandre Luiz Souto [UNESP] |
author_role |
author |
author2 |
Kukulka, Elisa Camargo [UNESP] Araújo, Juliani Caroline Ribeiro [UNESP] Campos, Tiago Moreira Bastos do Prado, Renata Falchete [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Thin, Gilmar Patrocínio Borges, Alexandre Luiz Souto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Praça Marechal Eduardo Gomes |
dc.contributor.author.fl_str_mv |
de Souza, Joyce Rodrigues [UNESP] Kukulka, Elisa Camargo [UNESP] Araújo, Juliani Caroline Ribeiro [UNESP] Campos, Tiago Moreira Bastos do Prado, Renata Falchete [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Thin, Gilmar Patrocínio Borges, Alexandre Luiz Souto [UNESP] |
dc.subject.por.fl_str_mv |
bioglass biopolymers bone regeneration cobalt polylactic acid strontium tissue engineering |
topic |
bioglass biopolymers bone regeneration cobalt polylactic acid strontium tissue engineering |
description |
The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol–gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro–Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal–Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T21:06:43Z 2023-03-01T21:06:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jbm.b.35141 Journal of Biomedical Materials Research - Part B Applied Biomaterials. 1552-4981 1552-4973 http://hdl.handle.net/11449/241500 10.1002/jbm.b.35141 2-s2.0-85135804651 |
url |
http://dx.doi.org/10.1002/jbm.b.35141 http://hdl.handle.net/11449/241500 |
identifier_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials. 1552-4981 1552-4973 10.1002/jbm.b.35141 2-s2.0-85135804651 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129067742920704 |