Streptococcus mutans attachment on a cast titanium surface

• Autor(a) principal: da Rocha, Sicknan Soares
• Data de Publicação: 2009
• Outros Autores: Bernardi, Adilson César Abreu [UNESP], Pizzolitto, Antônio Carlos [UNESP], Adabo, Gelson Luis [UNESP], Pizzolitto, Elisabeth Loshchagin [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1516-14392009000100003; http://hdl.handle.net/11449/71108
• topic: Bacterial adhesion; Biofilm; Surface roughness; Titanium; Alumina paste; Biofilm formation; Commercially Pure titaniums; Culture medium; Mueller; Rematitan systems; S. mutans; SEM; Streptococcus mutans; Surface corrosion; Titanium surfaces; Adhesion; Bacteriology; Biofilms; Biofilters; Corrosion; Metal recovery; Scanning electron microscopy; Surface properties

This study examined by means of scanning electron microscopy (SEM), the attachment of Streptococcus mutans and the corrosion of cast commercially pure titanium, used in dental dentures. The sample discs were cast in commercially pure titanium using the vacuum-pressure machine (Rematitan System). The surfaces of each metal were ground and polished with sandpaper (#300-4000) and alumina paste (0.3 μm). The roughness of the surface (Ra) was measured using the Surfcorder rugosimeter SE 1700. Four coupons were inserted separately into Falcon tubes contained Mueller Hinton broth inoculated with S. mutans ATCC 25175 (109 cuf) and incubated at 37 °C. The culture medium was changed every three days during a 365-day period, after which the falcons were prepared for observations by SEM. The mean Ra value of CP Ti was 0.1527 μm. After S. mutans biofilm removal, pits of corrosion were observed. Despite the low roughness, S. mutans attachment and biofilm formation was observed, which induced a surface corrosion of the cast pure titanium.