Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.prosdent.2019.11.024 http://hdl.handle.net/11449/200037 |
Resumo: | Statement of problem: Changes in physicochemical properties because of implant material aging and natural deterioration in the oral environment can facilitate microbial colonization and disturb the soft-tissue seal between the implant surfaces. Purpose: The purpose of this in vitro study was to investigate the effect of aging time on the physicochemical profile of titanium (Ti) and zirconia (ZrO2) implant materials. Further microbiology and cell analyses were used to provide insights into the physicochemical implications of biological behavior. Material and methods: Disk-shaped specimens of Ti and ZrO2 were submitted to roughness, morphology, and surface free energy (SFE) analyses before nonaging (NA) and after the aging process (A). To simulate natural aging, disks were subjected to low-temperature degradation (LTD) by using an autoclave at 134 ºC and 0.2 MPa pressure for 20 hours. The biological activities of the Ti and ZrO2 surfaces were determined by analyzing Candida albicans (C. albicans) biofilms and human gingival fibroblast (HGF) cell proliferation. For the microbiology assays, a variance analysis method (ANOVA) was used with the Tukey post hoc test. For the evaluation of cellular proliferation, the Kruskal-Wallis test followed by Dunn multiple comparisons were used. Results: Ti nonaging (TNA) and ZrO2 nonaging (ZNA) disks displayed hydrophilic and lipophilic properties, and this effect was sustained after the aging process. Low-temperature degradation resulted in a modest change in intermolecular interaction, with 1.06-fold for TA and 1.10-fold for ZA. No difference in biofilm formation was observed between NA and A disks of the same material. After 48 hours, the viability of the attached HGF cells was very similar to that in the NA and A groups, regardless of the tested material. Conclusion: The changes in the physicochemical properties of Ti and ZrO2 induced by the aging process do not interfere with C. albicans biofilm formation and HGF cell attachment, even after long-term exposure. |
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Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfacesStatement of problem: Changes in physicochemical properties because of implant material aging and natural deterioration in the oral environment can facilitate microbial colonization and disturb the soft-tissue seal between the implant surfaces. Purpose: The purpose of this in vitro study was to investigate the effect of aging time on the physicochemical profile of titanium (Ti) and zirconia (ZrO2) implant materials. Further microbiology and cell analyses were used to provide insights into the physicochemical implications of biological behavior. Material and methods: Disk-shaped specimens of Ti and ZrO2 were submitted to roughness, morphology, and surface free energy (SFE) analyses before nonaging (NA) and after the aging process (A). To simulate natural aging, disks were subjected to low-temperature degradation (LTD) by using an autoclave at 134 ºC and 0.2 MPa pressure for 20 hours. The biological activities of the Ti and ZrO2 surfaces were determined by analyzing Candida albicans (C. albicans) biofilms and human gingival fibroblast (HGF) cell proliferation. For the microbiology assays, a variance analysis method (ANOVA) was used with the Tukey post hoc test. For the evaluation of cellular proliferation, the Kruskal-Wallis test followed by Dunn multiple comparisons were used. Results: Ti nonaging (TNA) and ZrO2 nonaging (ZNA) disks displayed hydrophilic and lipophilic properties, and this effect was sustained after the aging process. Low-temperature degradation resulted in a modest change in intermolecular interaction, with 1.06-fold for TA and 1.10-fold for ZA. No difference in biofilm formation was observed between NA and A disks of the same material. After 48 hours, the viability of the attached HGF cells was very similar to that in the NA and A groups, regardless of the tested material. Conclusion: The changes in the physicochemical properties of Ti and ZrO2 induced by the aging process do not interfere with C. albicans biofilm formation and HGF cell attachment, even after long-term exposure.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Masters graduate Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Postdoctoral Research Fellow Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Technical Assistant Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Assistant Professor Department of Dentistry Federal University of Santa Catarina (UFSC)Associate Professor Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Masters graduate Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Postdoctoral Research Fellow Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Technical Assistant Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Associate Professor Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade Federal de Santa Catarina (UFSC)da Rocha, José Francisco S.S. [UNESP]de Avila, Erica D. [UNESP]Rigolin, Maria Sílvia M. [UNESP]Barbugli, Paula A. [UNESP]Marin, Danny O.M.Mollo Junior, Francisco A. [UNESP]Jorge, Janaina H. [UNESP]2020-12-12T01:55:54Z2020-12-12T01:55:54Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.prosdent.2019.11.024Journal of Prosthetic Dentistry.0022-3913http://hdl.handle.net/11449/20003710.1016/j.prosdent.2019.11.0242-s2.0-85079045647Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Prosthetic Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T11:21:24Zoai:repositorio.unesp.br:11449/200037Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:23:10.791069Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
title |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
spellingShingle |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces da Rocha, José Francisco S.S. [UNESP] |
title_short |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
title_full |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
title_fullStr |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
title_full_unstemmed |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
title_sort |
Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces |
author |
da Rocha, José Francisco S.S. [UNESP] |
author_facet |
da Rocha, José Francisco S.S. [UNESP] de Avila, Erica D. [UNESP] Rigolin, Maria Sílvia M. [UNESP] Barbugli, Paula A. [UNESP] Marin, Danny O.M. Mollo Junior, Francisco A. [UNESP] Jorge, Janaina H. [UNESP] |
author_role |
author |
author2 |
de Avila, Erica D. [UNESP] Rigolin, Maria Sílvia M. [UNESP] Barbugli, Paula A. [UNESP] Marin, Danny O.M. Mollo Junior, Francisco A. [UNESP] Jorge, Janaina H. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
da Rocha, José Francisco S.S. [UNESP] de Avila, Erica D. [UNESP] Rigolin, Maria Sílvia M. [UNESP] Barbugli, Paula A. [UNESP] Marin, Danny O.M. Mollo Junior, Francisco A. [UNESP] Jorge, Janaina H. [UNESP] |
description |
Statement of problem: Changes in physicochemical properties because of implant material aging and natural deterioration in the oral environment can facilitate microbial colonization and disturb the soft-tissue seal between the implant surfaces. Purpose: The purpose of this in vitro study was to investigate the effect of aging time on the physicochemical profile of titanium (Ti) and zirconia (ZrO2) implant materials. Further microbiology and cell analyses were used to provide insights into the physicochemical implications of biological behavior. Material and methods: Disk-shaped specimens of Ti and ZrO2 were submitted to roughness, morphology, and surface free energy (SFE) analyses before nonaging (NA) and after the aging process (A). To simulate natural aging, disks were subjected to low-temperature degradation (LTD) by using an autoclave at 134 ºC and 0.2 MPa pressure for 20 hours. The biological activities of the Ti and ZrO2 surfaces were determined by analyzing Candida albicans (C. albicans) biofilms and human gingival fibroblast (HGF) cell proliferation. For the microbiology assays, a variance analysis method (ANOVA) was used with the Tukey post hoc test. For the evaluation of cellular proliferation, the Kruskal-Wallis test followed by Dunn multiple comparisons were used. Results: Ti nonaging (TNA) and ZrO2 nonaging (ZNA) disks displayed hydrophilic and lipophilic properties, and this effect was sustained after the aging process. Low-temperature degradation resulted in a modest change in intermolecular interaction, with 1.06-fold for TA and 1.10-fold for ZA. No difference in biofilm formation was observed between NA and A disks of the same material. After 48 hours, the viability of the attached HGF cells was very similar to that in the NA and A groups, regardless of the tested material. Conclusion: The changes in the physicochemical properties of Ti and ZrO2 induced by the aging process do not interfere with C. albicans biofilm formation and HGF cell attachment, even after long-term exposure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:55:54Z 2020-12-12T01:55:54Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.prosdent.2019.11.024 Journal of Prosthetic Dentistry. 0022-3913 http://hdl.handle.net/11449/200037 10.1016/j.prosdent.2019.11.024 2-s2.0-85079045647 |
url |
http://dx.doi.org/10.1016/j.prosdent.2019.11.024 http://hdl.handle.net/11449/200037 |
identifier_str_mv |
Journal of Prosthetic Dentistry. 0022-3913 10.1016/j.prosdent.2019.11.024 2-s2.0-85079045647 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Prosthetic Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128926155800576 |