Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/neup.12698 http://hdl.handle.net/11449/206887 |
Resumo: | Pilocytic astrocytoma (PA) is the most frequent solid neoplasm in childhood. It has a good 5-year overall survival (90% in childhood and 52% in adults). However, up to 20% of patients experience residual tumor growth, recurrence, and death. Although the main genetic alteration of PAs, including KIAA1549:BRAF fusion, involves chromosome 7q34, we previously found frequent loss in chr9q34.3 locus in a small subset of these tumors. Among the genes present in this locus, EGFL7 is related to poor prognosis in several tumor types. In this study, we aimed to assess EGFL7 expression through immunohistochemistry, and to evaluate its prognostic value in a series of 64 clinically and molecularly well-characterized pilocytic astrocytomas. We found high expression of EGFL7 in 71.9% of patients. Low EGFL7 expression was associated with older patients, the mean age mainly older than 11 years (P = 0.027). EGFL7 expression was not associated with presence of KIAA1549:BRAF fusion, BRAF mutation, FGFR1 mutation, nor FGFR1 duplication. Moreover, high EGFL7 expression was associated with high FGFR1 (P = 0.037) and 5′-deoxy-5′-methyltioadenosine phosphorylase (MTAP) (P = 0.005) expression, and with unfavorable outcome of patients (P = 0.047). Multivariate analysis revealed low EGFL7 expression related to older patients and high EGFL7 expression related to retained expression of MTAP. In addition, we found a borderline significance of unfavorable outcome and high EGFL7 expression. Finally, EGFL7 expression was not associated with overall or event-free survival of PA patients. Our findings point to EGFL7 expression as a novel candidate prognostic marker in PA, which should be further investigated. |
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Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomasEGFL7FGFR1immunohistochemistryMTAPpilocytic astrocytomaPilocytic astrocytoma (PA) is the most frequent solid neoplasm in childhood. It has a good 5-year overall survival (90% in childhood and 52% in adults). However, up to 20% of patients experience residual tumor growth, recurrence, and death. Although the main genetic alteration of PAs, including KIAA1549:BRAF fusion, involves chromosome 7q34, we previously found frequent loss in chr9q34.3 locus in a small subset of these tumors. Among the genes present in this locus, EGFL7 is related to poor prognosis in several tumor types. In this study, we aimed to assess EGFL7 expression through immunohistochemistry, and to evaluate its prognostic value in a series of 64 clinically and molecularly well-characterized pilocytic astrocytomas. We found high expression of EGFL7 in 71.9% of patients. Low EGFL7 expression was associated with older patients, the mean age mainly older than 11 years (P = 0.027). EGFL7 expression was not associated with presence of KIAA1549:BRAF fusion, BRAF mutation, FGFR1 mutation, nor FGFR1 duplication. Moreover, high EGFL7 expression was associated with high FGFR1 (P = 0.037) and 5′-deoxy-5′-methyltioadenosine phosphorylase (MTAP) (P = 0.005) expression, and with unfavorable outcome of patients (P = 0.047). Multivariate analysis revealed low EGFL7 expression related to older patients and high EGFL7 expression related to retained expression of MTAP. In addition, we found a borderline significance of unfavorable outcome and high EGFL7 expression. Finally, EGFL7 expression was not associated with overall or event-free survival of PA patients. Our findings point to EGFL7 expression as a novel candidate prognostic marker in PA, which should be further investigated.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Molecular Oncology Research Center Barretos Cancer HospitalBarretos School of Health Sciences Dr. Paulo Prata – FACISBDepartment of Radiation Oncology The Ohio State UniversityDepartment of Pathology Botucatu Medical School São Paulo State University-UNESPDepartment of Neurosurgery Barretos Cancer HospitalLife and Health Sciences Research Institute (ICVS) School of Medicine University of MinhoICVS/3B's – PT Government Associate LaboratoryDepartment of Pathology Botucatu Medical School São Paulo State University-UNESPFAPESP: 2012/19590-0FAPESP: 2016/21727-4FAPESP: 2016/23919-8FAPESP: 2017/09749-5CNPq: 472447/2013-0Barretos Cancer HospitalDr. Paulo Prata – FACISBThe Ohio State UniversityUniversidade Estadual Paulista (Unesp)University of MinhoICVS/3B's – PT Government Associate LaboratoryBrunhara, Bruno B.Becker, Aline P.Neder, LucianoGonçalves, Paola G. [UNESP]de Oliveira, Cristiane [UNESP]Clara, Carlos A.Reis, Rui M.Bidinotto, Lucas T. [UNESP]2021-06-25T10:45:30Z2021-06-25T10:45:30Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21-28http://dx.doi.org/10.1111/neup.12698Neuropathology, v. 41, n. 1, p. 21-28, 2021.1440-17890919-6544http://hdl.handle.net/11449/20688710.1111/neup.126982-s2.0-85096770608Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuropathologyinfo:eu-repo/semantics/openAccess2021-10-23T15:33:58Zoai:repositorio.unesp.br:11449/206887Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:33:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
title |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
spellingShingle |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas Brunhara, Bruno B. EGFL7 FGFR1 immunohistochemistry MTAP pilocytic astrocytoma |
title_short |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
title_full |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
title_fullStr |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
title_full_unstemmed |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
title_sort |
Evaluation of the prognostic potential of EGFL7 in pilocytic astrocytomas |
author |
Brunhara, Bruno B. |
author_facet |
Brunhara, Bruno B. Becker, Aline P. Neder, Luciano Gonçalves, Paola G. [UNESP] de Oliveira, Cristiane [UNESP] Clara, Carlos A. Reis, Rui M. Bidinotto, Lucas T. [UNESP] |
author_role |
author |
author2 |
Becker, Aline P. Neder, Luciano Gonçalves, Paola G. [UNESP] de Oliveira, Cristiane [UNESP] Clara, Carlos A. Reis, Rui M. Bidinotto, Lucas T. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Barretos Cancer Hospital Dr. Paulo Prata – FACISB The Ohio State University Universidade Estadual Paulista (Unesp) University of Minho ICVS/3B's – PT Government Associate Laboratory |
dc.contributor.author.fl_str_mv |
Brunhara, Bruno B. Becker, Aline P. Neder, Luciano Gonçalves, Paola G. [UNESP] de Oliveira, Cristiane [UNESP] Clara, Carlos A. Reis, Rui M. Bidinotto, Lucas T. [UNESP] |
dc.subject.por.fl_str_mv |
EGFL7 FGFR1 immunohistochemistry MTAP pilocytic astrocytoma |
topic |
EGFL7 FGFR1 immunohistochemistry MTAP pilocytic astrocytoma |
description |
Pilocytic astrocytoma (PA) is the most frequent solid neoplasm in childhood. It has a good 5-year overall survival (90% in childhood and 52% in adults). However, up to 20% of patients experience residual tumor growth, recurrence, and death. Although the main genetic alteration of PAs, including KIAA1549:BRAF fusion, involves chromosome 7q34, we previously found frequent loss in chr9q34.3 locus in a small subset of these tumors. Among the genes present in this locus, EGFL7 is related to poor prognosis in several tumor types. In this study, we aimed to assess EGFL7 expression through immunohistochemistry, and to evaluate its prognostic value in a series of 64 clinically and molecularly well-characterized pilocytic astrocytomas. We found high expression of EGFL7 in 71.9% of patients. Low EGFL7 expression was associated with older patients, the mean age mainly older than 11 years (P = 0.027). EGFL7 expression was not associated with presence of KIAA1549:BRAF fusion, BRAF mutation, FGFR1 mutation, nor FGFR1 duplication. Moreover, high EGFL7 expression was associated with high FGFR1 (P = 0.037) and 5′-deoxy-5′-methyltioadenosine phosphorylase (MTAP) (P = 0.005) expression, and with unfavorable outcome of patients (P = 0.047). Multivariate analysis revealed low EGFL7 expression related to older patients and high EGFL7 expression related to retained expression of MTAP. In addition, we found a borderline significance of unfavorable outcome and high EGFL7 expression. Finally, EGFL7 expression was not associated with overall or event-free survival of PA patients. Our findings point to EGFL7 expression as a novel candidate prognostic marker in PA, which should be further investigated. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:45:30Z 2021-06-25T10:45:30Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/neup.12698 Neuropathology, v. 41, n. 1, p. 21-28, 2021. 1440-1789 0919-6544 http://hdl.handle.net/11449/206887 10.1111/neup.12698 2-s2.0-85096770608 |
url |
http://dx.doi.org/10.1111/neup.12698 http://hdl.handle.net/11449/206887 |
identifier_str_mv |
Neuropathology, v. 41, n. 1, p. 21-28, 2021. 1440-1789 0919-6544 10.1111/neup.12698 2-s2.0-85096770608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neuropathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
21-28 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792962448818438144 |