Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Detalhes bibliográficos
Autor(a) principal: Cabriela Alexandre de Santis, Ana Claudia [UNESP]
Data de Publicação: 2014
Outros Autores: Herrera, Heitor Miraglia, Marques de Sousa, Keyla Cartens [UNESP], Gonçalves, Luiz Ricardo [UNESP], Baccarim Denardi, Nathani Cristina [UNESP], Domingos, Iara Helena, Vilela Campos, João Bosco, Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200231&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/130283
Resumo: Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.
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spelling Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, BrazilDetecção molecular de micoplasmas hemotróficos em gatos domiciliados e errantes em Campo Grande, estado do Mato Grosso do Sul, BrasilFeline hemoplasmosisHemoplasmasMolecular diagnosisCentral-western BrazilHemoplasmose felinaHemoplasmasDiagnóstico molecularCentro-oeste brasileiroHemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.Hemoplasmas são bactérias encontradas aderidas aos eritrócitos de felinos. A hemoplasmose felina está frequentemente associada a gatos velhos machos, sem raça definida e com acesso à rua. O presente estudo objetivou realizar a detecção molecular de espécies de hemoplasmas em gatos domiciliados e errantes em Campo Grande, estado do Mato Grosso do Sul (MS), Brasil. Entre janeiro/2013 e abril/2013, amostras de sangue foram colhidas de 151 gatos domésticos (65 errantes e 86 domiciliados) e avaliadas por PCR frente à presença de sequências do gene do 16S rRNA de hemoplasmas, seguidas de sequenciamento, análise pelo BLAST e análise filogenética. Os resultados deste estudo mostraram uma ocorrência de 36,4%. Vinte e três (15,2%) gatos mostraram-se positivos na PCR para ‘Candidatus Mycoplasma haemominutum’, 17 (11,2%) para Mycoplasma haemofelis, e 15 (9,9%) para ‘Candidatus Mycoplasma turicensis’. A co-infecção por dois ou três hemoplasmas ocorreu em 25 gatos (16,6%). Não foi observada diferença estatística significativa entre sexo e estilo de vida dos gatos amostrados e a presença de hemoplasmas. O estudo mostrou que diferentes espécies de hemoplasmas circulam na população de gatos (domiciliados e errantes) na cidade de Campo Grande, MS, Brasil. Sugere-se o diagnóstico diferencial para hemoplasmose felina em gatos que apresentam sinais clínicos inespecíficos de doença com manifestação sistêmica.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Católica Dom Bosco – UCDB, Campo Grande, MS, BrazilCentro de Controle de Zoonoses – CCZ, Campo Grande, MS, BrazilUniversidade Estadual Paulista – UNESP, Jaboticabal, SP, BrazilFAPESP: 2013/06016-6FAPESP: 2013/09889-0Brazilian Coll Veterinary ParasitologyUniversidade Estadual Paulista (Unesp)Universidade Católica Dom Bosco (UCDB)Centro de Controle de Zoonoses (CCZ)Cabriela Alexandre de Santis, Ana Claudia [UNESP]Herrera, Heitor MiragliaMarques de Sousa, Keyla Cartens [UNESP]Gonçalves, Luiz Ricardo [UNESP]Baccarim Denardi, Nathani Cristina [UNESP]Domingos, Iara HelenaVilela Campos, João BoscoMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2015-11-03T18:06:08Z2015-11-03T18:06:08Z2014-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-236application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200231&lng=en&nrm=iso&tlng=enRevista Brasileira De Parasitologia Veterinaria. Sao Paulo: Brazilian Coll Veterinary Parasitology, v. 23, n. 2, p. 231-236, 2014.1984-2961http://hdl.handle.net/11449/13028310.1590/S1984-29612014039S1984-29612014000200231WOS:000342986900017S1984-29612014000200231.pdf3254990612451836Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira De Parasitologia Veterinaria1.090info:eu-repo/semantics/openAccess2024-06-07T13:02:39Zoai:repositorio.unesp.br:11449/130283Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:29:56.329246Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
Detecção molecular de micoplasmas hemotróficos em gatos domiciliados e errantes em Campo Grande, estado do Mato Grosso do Sul, Brasil
title Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
spellingShingle Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
Cabriela Alexandre de Santis, Ana Claudia [UNESP]
Feline hemoplasmosis
Hemoplasmas
Molecular diagnosis
Central-western Brazil
Hemoplasmose felina
Hemoplasmas
Diagnóstico molecular
Centro-oeste brasileiro
title_short Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
title_full Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
title_fullStr Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
title_full_unstemmed Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
title_sort Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil
author Cabriela Alexandre de Santis, Ana Claudia [UNESP]
author_facet Cabriela Alexandre de Santis, Ana Claudia [UNESP]
Herrera, Heitor Miraglia
Marques de Sousa, Keyla Cartens [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Baccarim Denardi, Nathani Cristina [UNESP]
Domingos, Iara Helena
Vilela Campos, João Bosco
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 Herrera, Heitor Miraglia
Marques de Sousa, Keyla Cartens [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Baccarim Denardi, Nathani Cristina [UNESP]
Domingos, Iara Helena
Vilela Campos, João Bosco
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Católica Dom Bosco (UCDB)
Centro de Controle de Zoonoses (CCZ)
dc.contributor.author.fl_str_mv Cabriela Alexandre de Santis, Ana Claudia [UNESP]
Herrera, Heitor Miraglia
Marques de Sousa, Keyla Cartens [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Baccarim Denardi, Nathani Cristina [UNESP]
Domingos, Iara Helena
Vilela Campos, João Bosco
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv Feline hemoplasmosis
Hemoplasmas
Molecular diagnosis
Central-western Brazil
Hemoplasmose felina
Hemoplasmas
Diagnóstico molecular
Centro-oeste brasileiro
topic Feline hemoplasmosis
Hemoplasmas
Molecular diagnosis
Central-western Brazil
Hemoplasmose felina
Hemoplasmas
Diagnóstico molecular
Centro-oeste brasileiro
description Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
2015-11-03T18:06:08Z
2015-11-03T18:06:08Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200231&lng=en&nrm=iso&tlng=en
Revista Brasileira De Parasitologia Veterinaria. Sao Paulo: Brazilian Coll Veterinary Parasitology, v. 23, n. 2, p. 231-236, 2014.
1984-2961
http://hdl.handle.net/11449/130283
10.1590/S1984-29612014039
S1984-29612014000200231
WOS:000342986900017
S1984-29612014000200231.pdf
3254990612451836
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200231&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/130283
identifier_str_mv Revista Brasileira De Parasitologia Veterinaria. Sao Paulo: Brazilian Coll Veterinary Parasitology, v. 23, n. 2, p. 231-236, 2014.
1984-2961
10.1590/S1984-29612014039
S1984-29612014000200231
WOS:000342986900017
S1984-29612014000200231.pdf
3254990612451836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira De Parasitologia Veterinaria
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dc.format.none.fl_str_mv 231-236
application/pdf
dc.publisher.none.fl_str_mv Brazilian Coll Veterinary Parasitology
publisher.none.fl_str_mv Brazilian Coll Veterinary Parasitology
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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