In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-1627-2013 http://hdl.handle.net/11449/30801 |
Resumo: | Introduction ,,,,,Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. ,,,, ,,,, ,,,,,Methods ,,,,,Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. ,,,, ,,,, ,,,,,Results ,,,,,After incubation in the presence of virus, all samples of platelets showed HCV RNA. ,,,, ,,,, ,,,,,Conclusions ,,,,,The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association. |
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In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virusHCVPlateletsCD81Introduction ,,,,,Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. ,,,, ,,,, ,,,,,Methods ,,,,,Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. ,,,, ,,,, ,,,,,Results ,,,,,After incubation in the presence of virus, all samples of platelets showed HCV RNA. ,,,, ,,,, ,,,,,Conclusions ,,,,,The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association.Universidade Estadual Paulista -UNESP Faculdade de Medicina de Botucatu Laboratório de Biologia Molecular do Hemocentro de BotucatuUniversidade Federal da Bahia (UFBA). Hospital Universitário Prof. Edgard Santos Laboratório de Pesquisa em Doenças InfecciosasUniversidade Estadual Paulista - UNESP Faculdade de Medicina de Botucatu Departamento de Clínica MédicaUniversidade Estadual Paulista -UNESP Faculdade de Medicina de Botucatu Laboratório de Biologia Molecular do Hemocentro de BotucatuUniversidade Estadual Paulista - UNESP Faculdade de Medicina de Botucatu Departamento de Clínica MédicaSociedade Brasileira de Medicina Tropical - SBMTUniversidade Estadual Paulista (Unesp)Universidade Federal da Bahia (UFBA)Padovani, Juliana LaraCorvino, Silvia MariaDrexler, Jan FelixSilva, Giovanni Faria [UNESP]Pardini, Maria Ines de Moura Campos [UNESP]Grotto, Rejane Maria Tommasini [UNESP]2014-05-20T15:18:14Z2014-05-20T15:18:14Z2013-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article154-155application/pdfhttp://dx.doi.org/10.1590/0037-8682-1627-2013Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 154-155, 2013.0037-8682http://hdl.handle.net/11449/3080110.1590/0037-8682-1627-2013S0037-86822013000200154WOS:000319625400006S0037-86822013000200154.pdf6322604200510676461958833458208477884485643265850000-0002-4035-9486SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2024-08-14T17:21:45Zoai:repositorio.unesp.br:11449/30801Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:21:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
spellingShingle |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus Padovani, Juliana Lara HCV Platelets CD81 |
title_short |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_full |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_fullStr |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_full_unstemmed |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_sort |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
author |
Padovani, Juliana Lara |
author_facet |
Padovani, Juliana Lara Corvino, Silvia Maria Drexler, Jan Felix Silva, Giovanni Faria [UNESP] Pardini, Maria Ines de Moura Campos [UNESP] Grotto, Rejane Maria Tommasini [UNESP] |
author_role |
author |
author2 |
Corvino, Silvia Maria Drexler, Jan Felix Silva, Giovanni Faria [UNESP] Pardini, Maria Ines de Moura Campos [UNESP] Grotto, Rejane Maria Tommasini [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal da Bahia (UFBA) |
dc.contributor.author.fl_str_mv |
Padovani, Juliana Lara Corvino, Silvia Maria Drexler, Jan Felix Silva, Giovanni Faria [UNESP] Pardini, Maria Ines de Moura Campos [UNESP] Grotto, Rejane Maria Tommasini [UNESP] |
dc.subject.por.fl_str_mv |
HCV Platelets CD81 |
topic |
HCV Platelets CD81 |
description |
Introduction ,,,,,Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. ,,,, ,,,, ,,,,,Methods ,,,,,Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. ,,,, ,,,, ,,,,,Results ,,,,,After incubation in the presence of virus, all samples of platelets showed HCV RNA. ,,,, ,,,, ,,,,,Conclusions ,,,,,The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-02 2014-05-20T15:18:14Z 2014-05-20T15:18:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-1627-2013 Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 154-155, 2013. 0037-8682 http://hdl.handle.net/11449/30801 10.1590/0037-8682-1627-2013 S0037-86822013000200154 WOS:000319625400006 S0037-86822013000200154.pdf 6322604200510676 4619588334582084 7788448564326585 0000-0002-4035-9486 |
url |
http://dx.doi.org/10.1590/0037-8682-1627-2013 http://hdl.handle.net/11449/30801 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 154-155, 2013. 0037-8682 10.1590/0037-8682-1627-2013 S0037-86822013000200154 WOS:000319625400006 S0037-86822013000200154.pdf 6322604200510676 4619588334582084 7788448564326585 0000-0002-4035-9486 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical 1.358 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
154-155 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128100239671296 |