Effect of pre-analytical treatments on bovine milk acute phase proteins
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12917-016-0769-6 http://hdl.handle.net/11449/173287 |
Resumo: | Background: Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory. This is particularly important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible. Methods: Milk samples were treated by heating at 56 °C for 30 min or preserved by addition of either potassium dichromate or bronopol respectively. Haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were measured in the various treatment groups and in control samples which were not treated, using enzyme linked immunoassays. The concentrations of each APP were compared between treated and non-treated groups using the Wilcoxon signed ranks tests. Results: Heat treatment of samples was found to have a significant lowering effect on milk M-SAA3 and CRP but not Hp. The use of potassium dichromate and bronopol as preservatives in milk had no significant effects on milk Hp and M-SAA3 concentration but lowered milk CRP values compared to controls. Conclusions: The observed effects of heating and preservative use on milk APP should be taken into consideration when assaying samples which have undergone heat treatment as a result of international transfer regulations involving biological samples or samples needing chemical preservation prior to transport to laboratory. |
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Effect of pre-analytical treatments on bovine milk acute phase proteinsC-reactive proteinHaptoglobinMammary associated serum amyloid A3Milk preservativesThermal stabilityBackground: Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory. This is particularly important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible. Methods: Milk samples were treated by heating at 56 °C for 30 min or preserved by addition of either potassium dichromate or bronopol respectively. Haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were measured in the various treatment groups and in control samples which were not treated, using enzyme linked immunoassays. The concentrations of each APP were compared between treated and non-treated groups using the Wilcoxon signed ranks tests. Results: Heat treatment of samples was found to have a significant lowering effect on milk M-SAA3 and CRP but not Hp. The use of potassium dichromate and bronopol as preservatives in milk had no significant effects on milk Hp and M-SAA3 concentration but lowered milk CRP values compared to controls. Conclusions: The observed effects of heating and preservative use on milk APP should be taken into consideration when assaying samples which have undergone heat treatment as a result of international transfer regulations involving biological samples or samples needing chemical preservation prior to transport to laboratory.Institute of Biodiversity Animal Health and Comparative Medicine University of Glasgow, Bearsden RoadFCAV UNESP Department of Veterinary Clinics and SurgeryGBRC University Place University of Glasgow Institute of Infection Immunity and InflammationUniversity of Glasgow Veterinary Diagnostic Services School of Veterinary Medicine, Bearsden RoadDepartment of Veterinary Physiology and Pharmacology College of Veterinary Medicine Federal University of AgricultureFCAV UNESP Department of Veterinary Clinics and SurgeryUniversity of GlasgowUniversidade Estadual Paulista (Unesp)Immunity and InflammationSchool of Veterinary MedicineFederal University of AgricultureThomas, Funmilola C.Santana, Andre M. [UNESP]Waterston, MaryHaining, HayleyEckersall, Peter David2018-12-11T17:04:30Z2018-12-11T17:04:30Z2016-07-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12917-016-0769-6BMC Veterinary Research, v. 12, n. 1, 2016.1746-6148http://hdl.handle.net/11449/17328710.1186/s12917-016-0769-62-s2.0-849792556472-s2.0-84979255647.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research0,934info:eu-repo/semantics/openAccess2024-06-06T14:10:34Zoai:repositorio.unesp.br:11449/173287Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:33:23.886038Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
title |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
spellingShingle |
Effect of pre-analytical treatments on bovine milk acute phase proteins Thomas, Funmilola C. C-reactive protein Haptoglobin Mammary associated serum amyloid A3 Milk preservatives Thermal stability |
title_short |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
title_full |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
title_fullStr |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
title_full_unstemmed |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
title_sort |
Effect of pre-analytical treatments on bovine milk acute phase proteins |
author |
Thomas, Funmilola C. |
author_facet |
Thomas, Funmilola C. Santana, Andre M. [UNESP] Waterston, Mary Haining, Hayley Eckersall, Peter David |
author_role |
author |
author2 |
Santana, Andre M. [UNESP] Waterston, Mary Haining, Hayley Eckersall, Peter David |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of Glasgow Universidade Estadual Paulista (Unesp) Immunity and Inflammation School of Veterinary Medicine Federal University of Agriculture |
dc.contributor.author.fl_str_mv |
Thomas, Funmilola C. Santana, Andre M. [UNESP] Waterston, Mary Haining, Hayley Eckersall, Peter David |
dc.subject.por.fl_str_mv |
C-reactive protein Haptoglobin Mammary associated serum amyloid A3 Milk preservatives Thermal stability |
topic |
C-reactive protein Haptoglobin Mammary associated serum amyloid A3 Milk preservatives Thermal stability |
description |
Background: Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory. This is particularly important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible. Methods: Milk samples were treated by heating at 56 °C for 30 min or preserved by addition of either potassium dichromate or bronopol respectively. Haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were measured in the various treatment groups and in control samples which were not treated, using enzyme linked immunoassays. The concentrations of each APP were compared between treated and non-treated groups using the Wilcoxon signed ranks tests. Results: Heat treatment of samples was found to have a significant lowering effect on milk M-SAA3 and CRP but not Hp. The use of potassium dichromate and bronopol as preservatives in milk had no significant effects on milk Hp and M-SAA3 concentration but lowered milk CRP values compared to controls. Conclusions: The observed effects of heating and preservative use on milk APP should be taken into consideration when assaying samples which have undergone heat treatment as a result of international transfer regulations involving biological samples or samples needing chemical preservation prior to transport to laboratory. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-25 2018-12-11T17:04:30Z 2018-12-11T17:04:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12917-016-0769-6 BMC Veterinary Research, v. 12, n. 1, 2016. 1746-6148 http://hdl.handle.net/11449/173287 10.1186/s12917-016-0769-6 2-s2.0-84979255647 2-s2.0-84979255647.pdf |
url |
http://dx.doi.org/10.1186/s12917-016-0769-6 http://hdl.handle.net/11449/173287 |
identifier_str_mv |
BMC Veterinary Research, v. 12, n. 1, 2016. 1746-6148 10.1186/s12917-016-0769-6 2-s2.0-84979255647 2-s2.0-84979255647.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Veterinary Research 0,934 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129220185948160 |