Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.archoralbio.2017.06.031 http://hdl.handle.net/11449/178980 |
Summary: | Objectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48 h and exposed for 5 min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5 min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24 h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey's Test (P ≤ 0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity. |
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Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicityAnti-inflammatory activityAntimicrobial activityBiofilmsCytotoxicityGenotoxicityThymus vulgaris lObjectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48 h and exposed for 5 min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5 min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24 h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey's Test (P ≤ 0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Institute of Science and Technology Department of Biosciences and Oral DiagnosisUniversidade do Vale do Paraíba (UNIVAP) Institute of Research and DevelopmentSão Paulo State University (UNESP) Institute of Science and Technology Department of Biosciences and Oral DiagnosisFAPESP: 2015/08776-3Universidade Estadual Paulista (Unesp)Institute of Research and DevelopmentOliveira, Jonatas Rafael de [UNESP]de Jesus Viegas, Daiane [UNESP]Martins, Ana Paula Réquia [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Soares, Cristina PachecoCamargo, Samira Esteves Afonso [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]de Oliveira, Luciane Dias [UNESP]2018-12-11T17:33:01Z2018-12-11T17:33:01Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article271-279application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.06.031Archives of Oral Biology, v. 82, p. 271-279.1879-15060003-9969http://hdl.handle.net/11449/17898010.1016/j.archoralbio.2017.06.0312-s2.0-850216655192-s2.0-85021665519.pdf93046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2023-10-17T06:07:11Zoai:repositorio.unesp.br:11449/178980Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:35.580881Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
title |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
spellingShingle |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity Oliveira, Jonatas Rafael de [UNESP] Anti-inflammatory activity Antimicrobial activity Biofilms Cytotoxicity Genotoxicity Thymus vulgaris l |
title_short |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
title_full |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
title_fullStr |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
title_full_unstemmed |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
title_sort |
Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity |
author |
Oliveira, Jonatas Rafael de [UNESP] |
author_facet |
Oliveira, Jonatas Rafael de [UNESP] de Jesus Viegas, Daiane [UNESP] Martins, Ana Paula Réquia [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Soares, Cristina Pacheco Camargo, Samira Esteves Afonso [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
de Jesus Viegas, Daiane [UNESP] Martins, Ana Paula Réquia [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Soares, Cristina Pacheco Camargo, Samira Esteves Afonso [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute of Research and Development |
dc.contributor.author.fl_str_mv |
Oliveira, Jonatas Rafael de [UNESP] de Jesus Viegas, Daiane [UNESP] Martins, Ana Paula Réquia [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Soares, Cristina Pacheco Camargo, Samira Esteves Afonso [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] de Oliveira, Luciane Dias [UNESP] |
dc.subject.por.fl_str_mv |
Anti-inflammatory activity Antimicrobial activity Biofilms Cytotoxicity Genotoxicity Thymus vulgaris l |
topic |
Anti-inflammatory activity Antimicrobial activity Biofilms Cytotoxicity Genotoxicity Thymus vulgaris l |
description |
Objectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48 h and exposed for 5 min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5 min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24 h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey's Test (P ≤ 0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:33:01Z 2018-12-11T17:33:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2017.06.031 Archives of Oral Biology, v. 82, p. 271-279. 1879-1506 0003-9969 http://hdl.handle.net/11449/178980 10.1016/j.archoralbio.2017.06.031 2-s2.0-85021665519 2-s2.0-85021665519.pdf 9304600166583100 0000-0003-0987-5594 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2017.06.031 http://hdl.handle.net/11449/178980 |
identifier_str_mv |
Archives of Oral Biology, v. 82, p. 271-279. 1879-1506 0003-9969 10.1016/j.archoralbio.2017.06.031 2-s2.0-85021665519 2-s2.0-85021665519.pdf 9304600166583100 0000-0003-0987-5594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
271-279 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128474475397120 |