Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.karger.com/Article/FullText/367848 http://hdl.handle.net/11449/129891 |
Resumo: | Background/Aims: There is disagreement regarding the performance of conventional optical microscopy to assess the origin of hematuria. The aim of this study was to determine the optimal cutoff point for dysmorphic cells in order to detect glomerular hematuria by optical and phase-contrast microscopy. Methods: In total, 131 urine samples (66 from patients with glomerulopathies and 65 from nephrolithiasis patients) were evaluated in a blinded fashion. The percentages of doughnut cells and acanthocytes were verified by optical and phase-contrast microscopy. A total of 131 patients were randomly allocated to the derivation (n = 73) and validation (n = 58) groups. Receiver-operating characteristic (ROC) curves were plotted to check the discriminatory power of each group and the best cutoff points were determined by the Youden index in the derivation group and subsequently tested in the validation group. Results: All areas under the ROC curve (AUCs) were statistically significant using both methods (conventional optical and phasecontrast microscopy) and both groups (derivation and validation). AUCs did not differ between different glomerulopathies. The best cutoff point to determine the glomerular origin of hematuria by total dysmorphic cells was 22% using an optical conventional microscope and 40% by phase-contrast microscopy. Conclusion: We determined the best cutoff points to interpret erythrocyte dysmorphism and demonstrated that it is possible to discriminate the origin of hematuria by evaluating erythrocyte dysmorphism in urinalysis using either an optical or a phase-contrast microscope. (C) 2014 S. Karger AG, Basel |
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Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuriaDysmorphic cellsErythrocyte dysmorphismGlomerular hematuriaBackground/Aims: There is disagreement regarding the performance of conventional optical microscopy to assess the origin of hematuria. The aim of this study was to determine the optimal cutoff point for dysmorphic cells in order to detect glomerular hematuria by optical and phase-contrast microscopy. Methods: In total, 131 urine samples (66 from patients with glomerulopathies and 65 from nephrolithiasis patients) were evaluated in a blinded fashion. The percentages of doughnut cells and acanthocytes were verified by optical and phase-contrast microscopy. A total of 131 patients were randomly allocated to the derivation (n = 73) and validation (n = 58) groups. Receiver-operating characteristic (ROC) curves were plotted to check the discriminatory power of each group and the best cutoff points were determined by the Youden index in the derivation group and subsequently tested in the validation group. Results: All areas under the ROC curve (AUCs) were statistically significant using both methods (conventional optical and phasecontrast microscopy) and both groups (derivation and validation). AUCs did not differ between different glomerulopathies. The best cutoff point to determine the glomerular origin of hematuria by total dysmorphic cells was 22% using an optical conventional microscope and 40% by phase-contrast microscopy. Conclusion: We determined the best cutoff points to interpret erythrocyte dysmorphism and demonstrated that it is possible to discriminate the origin of hematuria by evaluating erythrocyte dysmorphism in urinalysis using either an optical or a phase-contrast microscope. (C) 2014 S. Karger AG, BaselFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Botucatu Med Sch, Div Nephrol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Div Clin Pathol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Biostat, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Div Nephrol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Div Clin Pathol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Biostat, Botucatu, SP, BrazilFAPESP: 2010/11591-1FAPESP: 2010/14686-3KargerUniversidade Estadual Paulista (Unesp)Martinez, Marila Gaste [UNESP]Silva, Vanessa dos S. [UNESP]Valle, Adriana P. do [UNESP]Amaro, Carmen R. P. R. [UNESP]Corrente, Jose E. [UNESP]Martin, Luis Cuadrado [UNESP]2015-11-03T15:27:35Z2015-11-03T15:27:35Z2014-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article88-94http://www.karger.com/Article/FullText/367848Nephron Clinical Practice. Basel: Karger, v. 128, n. 1-2, p. 88-94, 2014.1660-2110http://hdl.handle.net/11449/12989110.1159/000367848WOS:000347919800013800393192613746049232031684466150000-0001-5478-49960000-0002-0090-8524Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNephron Clinical Practice1,093info:eu-repo/semantics/openAccess2024-08-14T17:23:33Zoai:repositorio.unesp.br:11449/129891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
title |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
spellingShingle |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria Martinez, Marila Gaste [UNESP] Dysmorphic cells Erythrocyte dysmorphism Glomerular hematuria |
title_short |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
title_full |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
title_fullStr |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
title_full_unstemmed |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
title_sort |
Comparison of different methods of erythrocyte dysmorphism analysis to determine the origin of hematuria |
author |
Martinez, Marila Gaste [UNESP] |
author_facet |
Martinez, Marila Gaste [UNESP] Silva, Vanessa dos S. [UNESP] Valle, Adriana P. do [UNESP] Amaro, Carmen R. P. R. [UNESP] Corrente, Jose E. [UNESP] Martin, Luis Cuadrado [UNESP] |
author_role |
author |
author2 |
Silva, Vanessa dos S. [UNESP] Valle, Adriana P. do [UNESP] Amaro, Carmen R. P. R. [UNESP] Corrente, Jose E. [UNESP] Martin, Luis Cuadrado [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martinez, Marila Gaste [UNESP] Silva, Vanessa dos S. [UNESP] Valle, Adriana P. do [UNESP] Amaro, Carmen R. P. R. [UNESP] Corrente, Jose E. [UNESP] Martin, Luis Cuadrado [UNESP] |
dc.subject.por.fl_str_mv |
Dysmorphic cells Erythrocyte dysmorphism Glomerular hematuria |
topic |
Dysmorphic cells Erythrocyte dysmorphism Glomerular hematuria |
description |
Background/Aims: There is disagreement regarding the performance of conventional optical microscopy to assess the origin of hematuria. The aim of this study was to determine the optimal cutoff point for dysmorphic cells in order to detect glomerular hematuria by optical and phase-contrast microscopy. Methods: In total, 131 urine samples (66 from patients with glomerulopathies and 65 from nephrolithiasis patients) were evaluated in a blinded fashion. The percentages of doughnut cells and acanthocytes were verified by optical and phase-contrast microscopy. A total of 131 patients were randomly allocated to the derivation (n = 73) and validation (n = 58) groups. Receiver-operating characteristic (ROC) curves were plotted to check the discriminatory power of each group and the best cutoff points were determined by the Youden index in the derivation group and subsequently tested in the validation group. Results: All areas under the ROC curve (AUCs) were statistically significant using both methods (conventional optical and phasecontrast microscopy) and both groups (derivation and validation). AUCs did not differ between different glomerulopathies. The best cutoff point to determine the glomerular origin of hematuria by total dysmorphic cells was 22% using an optical conventional microscope and 40% by phase-contrast microscopy. Conclusion: We determined the best cutoff points to interpret erythrocyte dysmorphism and demonstrated that it is possible to discriminate the origin of hematuria by evaluating erythrocyte dysmorphism in urinalysis using either an optical or a phase-contrast microscope. (C) 2014 S. Karger AG, Basel |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 2015-11-03T15:27:35Z 2015-11-03T15:27:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.karger.com/Article/FullText/367848 Nephron Clinical Practice. Basel: Karger, v. 128, n. 1-2, p. 88-94, 2014. 1660-2110 http://hdl.handle.net/11449/129891 10.1159/000367848 WOS:000347919800013 8003931926137460 4923203168446615 0000-0001-5478-4996 0000-0002-0090-8524 |
url |
http://www.karger.com/Article/FullText/367848 http://hdl.handle.net/11449/129891 |
identifier_str_mv |
Nephron Clinical Practice. Basel: Karger, v. 128, n. 1-2, p. 88-94, 2014. 1660-2110 10.1159/000367848 WOS:000347919800013 8003931926137460 4923203168446615 0000-0001-5478-4996 0000-0002-0090-8524 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nephron Clinical Practice 1,093 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
88-94 |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128168905670656 |