CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2002001100011 http://hdl.handle.net/11449/7963 |
Resumo: | The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The frequency of the CCR5 mutant allele (delta32ccr5) in this population was 0.032; however, no delta32ccr5 homozygote was detected. These results could be related to the intense ethnic admixture of the Brazilian population. There was no correlation between circulating ß-chemokines (MIP-1alpha, RANTES) and viral load in HIV-infected individuals. RANTES concentrations in plasma samples from HIV+ patients carrying the homozygous CCR5 allele (CCR5/CCR5) (28.23 ng/ml) were higher than in the control samples (16.07 ng/ml; P<0.05); however, this HIV+ patient group (mean 26.23 pg/ml) had significantly lower concentrations of MIP-1alpha than those observed in control samples (mean 31.20 pg/ml; P<0.05). Both HIV-1-infected and uninfected individuals heterozygous for the delta32ccr5 allele had significantly lower concentrations of circulating RANTES (mean 16.07 and 6.11 ng/ml, respectively) than CCR5/CCR5 individuals (mean 28.23 and 16.07 ng/ml, respectively; P<0.05). These findings suggest that the CCR5 allele and ß-chemokine production may affect the immunopathogenesis of HIV-1. |
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CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individualsHIV-1CC chemokine receptor 5delta32ccr5 Frequency alleleChemokinesThe 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The frequency of the CCR5 mutant allele (delta32ccr5) in this population was 0.032; however, no delta32ccr5 homozygote was detected. These results could be related to the intense ethnic admixture of the Brazilian population. There was no correlation between circulating ß-chemokines (MIP-1alpha, RANTES) and viral load in HIV-infected individuals. RANTES concentrations in plasma samples from HIV+ patients carrying the homozygous CCR5 allele (CCR5/CCR5) (28.23 ng/ml) were higher than in the control samples (16.07 ng/ml; P<0.05); however, this HIV+ patient group (mean 26.23 pg/ml) had significantly lower concentrations of MIP-1alpha than those observed in control samples (mean 31.20 pg/ml; P<0.05). Both HIV-1-infected and uninfected individuals heterozygous for the delta32ccr5 allele had significantly lower concentrations of circulating RANTES (mean 16.07 and 6.11 ng/ml, respectively) than CCR5/CCR5 individuals (mean 28.23 and 16.07 ng/ml, respectively; P<0.05). These findings suggest that the CCR5 allele and ß-chemokine production may affect the immunopathogenesis of HIV-1.Universidade Estadual Paulista Instituto de Química Pós-Graduação em BiotecnologiaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasUniversidade Estadual Paulista Instituto de Química Pós-Graduação em BiotecnologiaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Mikawa, A. Y. [UNESP]Tagliavini, S. A. [UNESP]Costa, P. I. [UNESP]2014-05-20T13:25:10Z2014-05-20T13:25:10Z2002-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1333-1337application/pdfhttp://dx.doi.org/10.1590/S0100-879X2002001100011Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 11, p. 1333-1337, 2002.0100-879Xhttp://hdl.handle.net/11449/796310.1590/S0100-879X2002001100011S0100-879X2002001100011WOS:000179717100011S0100-879X2002001100011.pdf67202237159173810000-0002-3350-8308SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/7963Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:38.186191Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
title |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
spellingShingle |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals Mikawa, A. Y. [UNESP] HIV-1 CC chemokine receptor 5 delta32ccr5 Frequency allele Chemokines |
title_short |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
title_full |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
title_fullStr |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
title_full_unstemmed |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
title_sort |
CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
author |
Mikawa, A. Y. [UNESP] |
author_facet |
Mikawa, A. Y. [UNESP] Tagliavini, S. A. [UNESP] Costa, P. I. [UNESP] |
author_role |
author |
author2 |
Tagliavini, S. A. [UNESP] Costa, P. I. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mikawa, A. Y. [UNESP] Tagliavini, S. A. [UNESP] Costa, P. I. [UNESP] |
dc.subject.por.fl_str_mv |
HIV-1 CC chemokine receptor 5 delta32ccr5 Frequency allele Chemokines |
topic |
HIV-1 CC chemokine receptor 5 delta32ccr5 Frequency allele Chemokines |
description |
The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The frequency of the CCR5 mutant allele (delta32ccr5) in this population was 0.032; however, no delta32ccr5 homozygote was detected. These results could be related to the intense ethnic admixture of the Brazilian population. There was no correlation between circulating ß-chemokines (MIP-1alpha, RANTES) and viral load in HIV-infected individuals. RANTES concentrations in plasma samples from HIV+ patients carrying the homozygous CCR5 allele (CCR5/CCR5) (28.23 ng/ml) were higher than in the control samples (16.07 ng/ml; P<0.05); however, this HIV+ patient group (mean 26.23 pg/ml) had significantly lower concentrations of MIP-1alpha than those observed in control samples (mean 31.20 pg/ml; P<0.05). Both HIV-1-infected and uninfected individuals heterozygous for the delta32ccr5 allele had significantly lower concentrations of circulating RANTES (mean 16.07 and 6.11 ng/ml, respectively) than CCR5/CCR5 individuals (mean 28.23 and 16.07 ng/ml, respectively; P<0.05). These findings suggest that the CCR5 allele and ß-chemokine production may affect the immunopathogenesis of HIV-1. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-11-01 2014-05-20T13:25:10Z 2014-05-20T13:25:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2002001100011 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 11, p. 1333-1337, 2002. 0100-879X http://hdl.handle.net/11449/7963 10.1590/S0100-879X2002001100011 S0100-879X2002001100011 WOS:000179717100011 S0100-879X2002001100011.pdf 6720223715917381 0000-0002-3350-8308 |
url |
http://dx.doi.org/10.1590/S0100-879X2002001100011 http://hdl.handle.net/11449/7963 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 11, p. 1333-1337, 2002. 0100-879X 10.1590/S0100-879X2002001100011 S0100-879X2002001100011 WOS:000179717100011 S0100-879X2002001100011.pdf 6720223715917381 0000-0002-3350-8308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research 1.492 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1333-1337 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128477723885568 |