Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjm.2017.01.012 http://hdl.handle.net/11449/163451 |
Resumo: | Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE Delta motB) or non-flagellated (SE Delta fliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE Delta motB and SE Delta fliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE Delta motB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE Delta fliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. |
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Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chickenMutationFlagellumVirulence factorsPoultryGut colonisationSalmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE Delta motB) or non-flagellated (SE Delta fliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE Delta motB and SE Delta fliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE Delta motB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE Delta fliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP, BrazilUniv Fed Paraiba, Dept Ciencias Vet, Areia, Paraiba, BrazilUniv Nottingham, Sch Vet Med & Sci, Sutton Bonington Campus, Nottingham, Leics, EnglandUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP, BrazilFAPESP: 2014/02014-1FAPESP: 2013/26127-7Soc Brasileira MicrobiologiaUniversidade Estadual Paulista (Unesp)Univ Fed ParaibaUniv NottinghamBarbosa, Fernanda de Oliveira [UNESP]Freitas Neto, Oliveiro Caetano deAlves Batista, Diego Felipe [UNESP]Almeida, Adriana Maria de [UNESP]Rubio, Marcela da Silva [UNESP]Rodrigues Alves, Lucas Bocchini [UNESP]Vasconcelos, Rosemeire de Oliveira [UNESP]Barrow, Paul AndrewBerchieri Junior, Angelo [UNESP]2018-11-26T17:42:05Z2018-11-26T17:42:05Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article754-759application/pdfhttp://dx.doi.org/10.1016/j.bjm.2017.01.012Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 4, p. 754-759, 2017.1517-8382http://hdl.handle.net/11449/16345110.1016/j.bjm.2017.01.012S1517-83822017000400754WOS:000414697800020S1517-83822017000400754.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiology0,630info:eu-repo/semantics/openAccess2024-06-07T13:03:09Zoai:repositorio.unesp.br:11449/163451Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:32:08.688101Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
title |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
spellingShingle |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken Barbosa, Fernanda de Oliveira [UNESP] Mutation Flagellum Virulence factors Poultry Gut colonisation |
title_short |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
title_full |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
title_fullStr |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
title_full_unstemmed |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
title_sort |
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken |
author |
Barbosa, Fernanda de Oliveira [UNESP] |
author_facet |
Barbosa, Fernanda de Oliveira [UNESP] Freitas Neto, Oliveiro Caetano de Alves Batista, Diego Felipe [UNESP] Almeida, Adriana Maria de [UNESP] Rubio, Marcela da Silva [UNESP] Rodrigues Alves, Lucas Bocchini [UNESP] Vasconcelos, Rosemeire de Oliveira [UNESP] Barrow, Paul Andrew Berchieri Junior, Angelo [UNESP] |
author_role |
author |
author2 |
Freitas Neto, Oliveiro Caetano de Alves Batista, Diego Felipe [UNESP] Almeida, Adriana Maria de [UNESP] Rubio, Marcela da Silva [UNESP] Rodrigues Alves, Lucas Bocchini [UNESP] Vasconcelos, Rosemeire de Oliveira [UNESP] Barrow, Paul Andrew Berchieri Junior, Angelo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Fed Paraiba Univ Nottingham |
dc.contributor.author.fl_str_mv |
Barbosa, Fernanda de Oliveira [UNESP] Freitas Neto, Oliveiro Caetano de Alves Batista, Diego Felipe [UNESP] Almeida, Adriana Maria de [UNESP] Rubio, Marcela da Silva [UNESP] Rodrigues Alves, Lucas Bocchini [UNESP] Vasconcelos, Rosemeire de Oliveira [UNESP] Barrow, Paul Andrew Berchieri Junior, Angelo [UNESP] |
dc.subject.por.fl_str_mv |
Mutation Flagellum Virulence factors Poultry Gut colonisation |
topic |
Mutation Flagellum Virulence factors Poultry Gut colonisation |
description |
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE Delta motB) or non-flagellated (SE Delta fliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE Delta motB and SE Delta fliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE Delta motB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE Delta fliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-11-26T17:42:05Z 2018-11-26T17:42:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjm.2017.01.012 Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 4, p. 754-759, 2017. 1517-8382 http://hdl.handle.net/11449/163451 10.1016/j.bjm.2017.01.012 S1517-83822017000400754 WOS:000414697800020 S1517-83822017000400754.pdf |
url |
http://dx.doi.org/10.1016/j.bjm.2017.01.012 http://hdl.handle.net/11449/163451 |
identifier_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 4, p. 754-759, 2017. 1517-8382 10.1016/j.bjm.2017.01.012 S1517-83822017000400754 WOS:000414697800020 S1517-83822017000400754.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
754-759 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129529793740800 |