Efeitos do FK-506 na regeneração de nervos após neurorrafia láteo-terminal: estudo em ratos

• Autor(a) principal: Dolfini, Maria Inês Meira [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/124136; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/12-06-2015/000831507.pdf
• topic: Musculos - Regeneração; Nervos perifericos; Sistema nervoso - Cirurgia; Muscles - Regeneration; Nervous system - Diseases

The latero-terminal neurorrhaphy without injury to the donor nerve, represented great advances in microsurgery of peripheral nerves. The receptor nerve distal stump was sutured on the lateral side of the donor nerve, without harming it. Since 1994, FK-506, an immunosupressive drug, has been used to prevent allograft rejection in organ transplants and presented, in some studies, as a secondary action, positive effects on peripheral nerve regeneration. The aim of this work was to investigate whether the administration of FK-506 would benefit nerve regeneration after a latero-terminal neurorrhaphy. We used 80 Wistar rats, divided into four experimental groups. Each rat had its left fibular nerve sectioned and the distal stump sutured to the lateral of the tibial nerve. The animals of group I, II and III were subjected to the administration of FK-506 in an amount of 1.0, 0.5 to 0.25mg/K/day, respectively, for two months, while the aminals of the control group received no drug. Two months after surgery the rats were submitted to the walking test, eletrophysiological study and analysis of muscle and nerve fibers. The group II (GII) showed cranial tibial muscle (MTC) with lower mass (p < 0.05) and higher amplitude (p = 0.019). The results by the total number of nerve fibers was higher for GII (p < 0.001) . There was no significant difference between groups for functional indices. Based on our experimental model, we could conclude that the administration of 0.5 mg/Kg/dia of FK-506 decreased body mass gain and mass of MTC and increased regeneration of nerve fibers, although not able to change the functional response