Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/128009 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/16-09-2015/000848868.pdf |
Resumo: | In humans, the immunosuppression observed in uremic patients is associated with the neutrophil dysfunction caused by uremic toxins. P-cresol accumulates in the blood of patients with chronic renal failure (CRF) promoting the inhibition of neutrophil oxidative metabolism. This study aimed to measure the plasma concentrations of p-cresol in dogs with CRF and test the hypothesis that free p-cresol causes neutrophil dysfunction. To this end, plasma concentration of p-cresol was determined using high pressure liquid chromatography (HPLC) in dogs presenting stage IV of CRF. The in vitro specific effect of p-cresol on the oxidative metabolism and apoptosis rate of neutrophils from healthy dogs was also evaluated, considering the highest plasma concentration of this toxin observed on the in vivo measurement of dogs with CRF. Isolated neutrophils from 20 healthy dogs were incubated in RPMI medium alone, supplemented with p-cresol or plasma from dogs with CRF. Neutrophil oxidative metabolism was assessed by capillary flow cytometry using the probes hydroethidine and 2',7'- dichlorofluorescein diacetate with or without stimulus with phorbol myristate acetate and N-formyl-methionyl-leucylphenylalanine. Apoptosis and viability of neutrophils were quantified also in capillary flow cytometry using Annexin V-PE system with or without stimulus with camptothecin. Dogs with CRF presented oxidative stress and increased plasma levels of p-cresol, the higher concentration of p-cresol caused in vitro neutrophil dysfunction. It can be concluded that p-cresol may be involved with in vivo oxidative stress that its higher concentration decreases the viability and lead to a reduced production of reactive oxygen species |
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Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cãesUremiaMorte celularLeucócitosCãoMetabolismoImunossupressãorespiratory burstIn humans, the immunosuppression observed in uremic patients is associated with the neutrophil dysfunction caused by uremic toxins. P-cresol accumulates in the blood of patients with chronic renal failure (CRF) promoting the inhibition of neutrophil oxidative metabolism. This study aimed to measure the plasma concentrations of p-cresol in dogs with CRF and test the hypothesis that free p-cresol causes neutrophil dysfunction. To this end, plasma concentration of p-cresol was determined using high pressure liquid chromatography (HPLC) in dogs presenting stage IV of CRF. The in vitro specific effect of p-cresol on the oxidative metabolism and apoptosis rate of neutrophils from healthy dogs was also evaluated, considering the highest plasma concentration of this toxin observed on the in vivo measurement of dogs with CRF. Isolated neutrophils from 20 healthy dogs were incubated in RPMI medium alone, supplemented with p-cresol or plasma from dogs with CRF. Neutrophil oxidative metabolism was assessed by capillary flow cytometry using the probes hydroethidine and 2',7'- dichlorofluorescein diacetate with or without stimulus with phorbol myristate acetate and N-formyl-methionyl-leucylphenylalanine. Apoptosis and viability of neutrophils were quantified also in capillary flow cytometry using Annexin V-PE system with or without stimulus with camptothecin. Dogs with CRF presented oxidative stress and increased plasma levels of p-cresol, the higher concentration of p-cresol caused in vitro neutrophil dysfunction. It can be concluded that p-cresol may be involved with in vivo oxidative stress that its higher concentration decreases the viability and lead to a reduced production of reactive oxygen speciesFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Ciarlini, Paulo Cesar [UNESP]Universidade Estadual Paulista (Unesp)Bosco, Anelise Maria [UNESP]2015-10-06T13:02:50Z2015-10-06T13:02:50Z2014-03-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis58 f.application/pdfBOSCO, Anelise Maria. Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães. 2014. 58 f. , 2014.http://hdl.handle.net/11449/128009000848868http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/16-09-2015/000848868.pdf33004021075P836139400182995000000-0003-1480-5208Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-18T06:27:31Zoai:repositorio.unesp.br:11449/128009Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:21:48.735515Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
title |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
spellingShingle |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães Bosco, Anelise Maria [UNESP] Uremia Morte celular Leucócitos Cão Metabolismo Imunossupressão respiratory burst |
title_short |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
title_full |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
title_fullStr |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
title_full_unstemmed |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
title_sort |
Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães |
author |
Bosco, Anelise Maria [UNESP] |
author_facet |
Bosco, Anelise Maria [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ciarlini, Paulo Cesar [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bosco, Anelise Maria [UNESP] |
dc.subject.por.fl_str_mv |
Uremia Morte celular Leucócitos Cão Metabolismo Imunossupressão respiratory burst |
topic |
Uremia Morte celular Leucócitos Cão Metabolismo Imunossupressão respiratory burst |
description |
In humans, the immunosuppression observed in uremic patients is associated with the neutrophil dysfunction caused by uremic toxins. P-cresol accumulates in the blood of patients with chronic renal failure (CRF) promoting the inhibition of neutrophil oxidative metabolism. This study aimed to measure the plasma concentrations of p-cresol in dogs with CRF and test the hypothesis that free p-cresol causes neutrophil dysfunction. To this end, plasma concentration of p-cresol was determined using high pressure liquid chromatography (HPLC) in dogs presenting stage IV of CRF. The in vitro specific effect of p-cresol on the oxidative metabolism and apoptosis rate of neutrophils from healthy dogs was also evaluated, considering the highest plasma concentration of this toxin observed on the in vivo measurement of dogs with CRF. Isolated neutrophils from 20 healthy dogs were incubated in RPMI medium alone, supplemented with p-cresol or plasma from dogs with CRF. Neutrophil oxidative metabolism was assessed by capillary flow cytometry using the probes hydroethidine and 2',7'- dichlorofluorescein diacetate with or without stimulus with phorbol myristate acetate and N-formyl-methionyl-leucylphenylalanine. Apoptosis and viability of neutrophils were quantified also in capillary flow cytometry using Annexin V-PE system with or without stimulus with camptothecin. Dogs with CRF presented oxidative stress and increased plasma levels of p-cresol, the higher concentration of p-cresol caused in vitro neutrophil dysfunction. It can be concluded that p-cresol may be involved with in vivo oxidative stress that its higher concentration decreases the viability and lead to a reduced production of reactive oxygen species |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-21 2015-10-06T13:02:50Z 2015-10-06T13:02:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BOSCO, Anelise Maria. Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães. 2014. 58 f. , 2014. http://hdl.handle.net/11449/128009 000848868 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/16-09-2015/000848868.pdf 33004021075P8 3613940018299500 0000-0003-1480-5208 |
identifier_str_mv |
BOSCO, Anelise Maria. Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães. 2014. 58 f. , 2014. 000848868 33004021075P8 3613940018299500 0000-0003-1480-5208 |
url |
http://hdl.handle.net/11449/128009 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/16-09-2015/000848868.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
58 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129510373064704 |