Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.reprotox.2016.06.019 http://hdl.handle.net/11449/173192 |
Resumo: | Exposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0 μg of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation. |
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Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation studyDioxinEpididymisFertilitySperm qualityTransgenerational effectExposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0 μg of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Reproductive and Developmental Biology and Toxicology Department of Morphology Institute of Biosciences of Botucatu UNESP—Univ Estadual, BotucatuDepartment of Morphology Stomatology and Physiology School of Dentistry USP—University of São Paulo, Ribeirão PretoLaboratory of Reproductive and Developmental Biology and Toxicology Department of Morphology Institute of Biosciences of Botucatu UNESP—Univ Estadual, BotucatuFAPESP: 2012/00365-6Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Sanabria, Marciana [UNESP]Cucielo, Maira Smaniotto [UNESP]Guerra, Marina Trevizan [UNESP]dos Santos Borges, Cibele [UNESP]Banzato, Thais Petrochelli [UNESP]Perobelli, Juliana Elaine [UNESP]Leite, Gabriel Adan Araújo [UNESP]Anselmo-Franci, Janete AparecidaDe Grava Kempinas, Wilma [UNESP]2018-12-11T17:04:04Z2018-12-11T17:04:04Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29-38application/pdfhttp://dx.doi.org/10.1016/j.reprotox.2016.06.019Reproductive Toxicology, v. 65, p. 29-38.1873-17080890-6238http://hdl.handle.net/11449/17319210.1016/j.reprotox.2016.06.0192-s2.0-849776140312-s2.0-84977614031.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Toxicology0,846info:eu-repo/semantics/openAccess2023-10-22T06:08:26Zoai:repositorio.unesp.br:11449/173192Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:37:35.547125Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
title |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
spellingShingle |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study Sanabria, Marciana [UNESP] Dioxin Epididymis Fertility Sperm quality Transgenerational effect |
title_short |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
title_full |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
title_fullStr |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
title_full_unstemmed |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
title_sort |
Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study |
author |
Sanabria, Marciana [UNESP] |
author_facet |
Sanabria, Marciana [UNESP] Cucielo, Maira Smaniotto [UNESP] Guerra, Marina Trevizan [UNESP] dos Santos Borges, Cibele [UNESP] Banzato, Thais Petrochelli [UNESP] Perobelli, Juliana Elaine [UNESP] Leite, Gabriel Adan Araújo [UNESP] Anselmo-Franci, Janete Aparecida De Grava Kempinas, Wilma [UNESP] |
author_role |
author |
author2 |
Cucielo, Maira Smaniotto [UNESP] Guerra, Marina Trevizan [UNESP] dos Santos Borges, Cibele [UNESP] Banzato, Thais Petrochelli [UNESP] Perobelli, Juliana Elaine [UNESP] Leite, Gabriel Adan Araújo [UNESP] Anselmo-Franci, Janete Aparecida De Grava Kempinas, Wilma [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sanabria, Marciana [UNESP] Cucielo, Maira Smaniotto [UNESP] Guerra, Marina Trevizan [UNESP] dos Santos Borges, Cibele [UNESP] Banzato, Thais Petrochelli [UNESP] Perobelli, Juliana Elaine [UNESP] Leite, Gabriel Adan Araújo [UNESP] Anselmo-Franci, Janete Aparecida De Grava Kempinas, Wilma [UNESP] |
dc.subject.por.fl_str_mv |
Dioxin Epididymis Fertility Sperm quality Transgenerational effect |
topic |
Dioxin Epididymis Fertility Sperm quality Transgenerational effect |
description |
Exposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0 μg of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-12-11T17:04:04Z 2018-12-11T17:04:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.reprotox.2016.06.019 Reproductive Toxicology, v. 65, p. 29-38. 1873-1708 0890-6238 http://hdl.handle.net/11449/173192 10.1016/j.reprotox.2016.06.019 2-s2.0-84977614031 2-s2.0-84977614031.pdf |
url |
http://dx.doi.org/10.1016/j.reprotox.2016.06.019 http://hdl.handle.net/11449/173192 |
identifier_str_mv |
Reproductive Toxicology, v. 65, p. 29-38. 1873-1708 0890-6238 10.1016/j.reprotox.2016.06.019 2-s2.0-84977614031 2-s2.0-84977614031.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Toxicology 0,846 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
29-38 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128541161684992 |