Latex–collagen membrane: an alternative treatment for tibial bone defects
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10853-022-08009-7 http://hdl.handle.net/11449/249012 |
Resumo: | Due to the difficulty of consolidation, the risk of fractures, and the high cost of therapy, the orthopedic field views the restoration of bony defects or post-traumatic diseases as a significant issue. In recent years, efforts have been made toward guided bone regeneration as a viable solution. In this study, natural rubber latex (NRL) and latex–collagen (NRL-COL) membranes were developed and characterized, and their effects on bone regeneration in rat tibial lesions were assessed. According to the physicochemical findings, the hydrolyzed COL was effectively incorporated into the NRL membrane to produce a more robust membrane (348% increase in stress) and showed good biocompatibility for human dermal fibroblast cells and in hemolytic activity assay. According to bone analysis using computed microtomography, a substantial difference in bone volume (BV) was found among NRL, control (CONT), and NRL-COL. NRL-COL exhibited the greatest value for the bone volume/total volume ratio (BV/TV). The presence of NRL and COL was significant for the analysis of BV and BV/TV, respectively. All the experimental groups demonstrated a higher bone quality and fracture resistance, as shown by the mechanical properties of the bone. In the COL group, both neoformation of bone and total collagen levels were greater, indicating that treated defects had been closed and mature bone cells were present. Due to its angiogenic properties and the low cost of NRL membrane production, the NRL-COL membrane could be a good substitute for upcoming bone regeneration applications. Graphical Abstract: [Figure not available: see fulltext.] |
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Latex–collagen membrane: an alternative treatment for tibial bone defectsDue to the difficulty of consolidation, the risk of fractures, and the high cost of therapy, the orthopedic field views the restoration of bony defects or post-traumatic diseases as a significant issue. In recent years, efforts have been made toward guided bone regeneration as a viable solution. In this study, natural rubber latex (NRL) and latex–collagen (NRL-COL) membranes were developed and characterized, and their effects on bone regeneration in rat tibial lesions were assessed. According to the physicochemical findings, the hydrolyzed COL was effectively incorporated into the NRL membrane to produce a more robust membrane (348% increase in stress) and showed good biocompatibility for human dermal fibroblast cells and in hemolytic activity assay. According to bone analysis using computed microtomography, a substantial difference in bone volume (BV) was found among NRL, control (CONT), and NRL-COL. NRL-COL exhibited the greatest value for the bone volume/total volume ratio (BV/TV). The presence of NRL and COL was significant for the analysis of BV and BV/TV, respectively. All the experimental groups demonstrated a higher bone quality and fracture resistance, as shown by the mechanical properties of the bone. In the COL group, both neoformation of bone and total collagen levels were greater, indicating that treated defects had been closed and mature bone cells were present. Due to its angiogenic properties and the low cost of NRL membrane production, the NRL-COL membrane could be a good substitute for upcoming bone regeneration applications. Graphical Abstract: [Figure not available: see fulltext.]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Foundation for the National Institutes of HealthFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Bioengineering Laboratory Ribeirão Preto Medical School São Paulo University (USP), SPDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), SPDepartment of Dental Materials and Prosthesis School of Dentistry of Ribeirão Preto São Paulo University (USP), SPDepartment of Morphology Physiology and Basic Pathology School of Dentistry of Ribeirão Preto São Paulo University (USP), SPTerasaki Institute for Biomedical InnovationBotucatu Medical School São Paulo State University (UNESP), SPNational Heart and Lung Institute Imperial College LondonUniversity Center of Hermínio Ometto Foundation (FHO), SPDivision of Dermatology Department of Internal Medicine Ribeirão Preto Medical School São Paulo University (USP), SPAutonomy Research Center for STEAHM (ARCS) California State UniversityDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), SPBotucatu Medical School São Paulo State University (UNESP), SPCAPES: 1713685Foundation for the National Institutes of Health: 1R01GM126571-01Foundation for the National Institutes of Health: 1U01CA214411-01A1FAPESP: 2011/17411-8FAPESP: 2014/17526-8)FAPESP: 2017/19603-8FAPESP: 2017/20051-0CNPq: 317203/2021-5Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Terasaki Institute for Biomedical InnovationImperial College LondonUniversity Center of Hermínio Ometto Foundation (FHO)California State Universityde Paiva, Marcela BrittoPegorin Brasil, Giovana Sant’Ana [UNESP]Chagas, Ana Laura Destro [UNESP]Macedo, Ana PaulaRamos, JúniaIssa, João Paulo MardeganGangrade, AnkitFloriano, Juliana Ferreira [UNESP]Caetano, Guilherme FerreiraLi, BingbingFarhadi, NedaMandal, KalpanaDokmeci, Mehmet RemziJucaud, VadimHerculano, Rondinelli Donizetti [UNESP]Shimano, Antonio Carlos2023-07-29T13:59:58Z2023-07-29T13:59:58Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22019-22041http://dx.doi.org/10.1007/s10853-022-08009-7Journal of Materials Science, v. 57, n. 48, p. 22019-22041, 2022.1573-48030022-2461http://hdl.handle.net/11449/24901210.1007/s10853-022-08009-72-s2.0-85143614000Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Scienceinfo:eu-repo/semantics/openAccess2024-08-14T17:22:37Zoai:repositorio.unesp.br:11449/249012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
title |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
spellingShingle |
Latex–collagen membrane: an alternative treatment for tibial bone defects de Paiva, Marcela Britto |
title_short |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
title_full |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
title_fullStr |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
title_full_unstemmed |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
title_sort |
Latex–collagen membrane: an alternative treatment for tibial bone defects |
author |
de Paiva, Marcela Britto |
author_facet |
de Paiva, Marcela Britto Pegorin Brasil, Giovana Sant’Ana [UNESP] Chagas, Ana Laura Destro [UNESP] Macedo, Ana Paula Ramos, Júnia Issa, João Paulo Mardegan Gangrade, Ankit Floriano, Juliana Ferreira [UNESP] Caetano, Guilherme Ferreira Li, Bingbing Farhadi, Neda Mandal, Kalpana Dokmeci, Mehmet Remzi Jucaud, Vadim Herculano, Rondinelli Donizetti [UNESP] Shimano, Antonio Carlos |
author_role |
author |
author2 |
Pegorin Brasil, Giovana Sant’Ana [UNESP] Chagas, Ana Laura Destro [UNESP] Macedo, Ana Paula Ramos, Júnia Issa, João Paulo Mardegan Gangrade, Ankit Floriano, Juliana Ferreira [UNESP] Caetano, Guilherme Ferreira Li, Bingbing Farhadi, Neda Mandal, Kalpana Dokmeci, Mehmet Remzi Jucaud, Vadim Herculano, Rondinelli Donizetti [UNESP] Shimano, Antonio Carlos |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Terasaki Institute for Biomedical Innovation Imperial College London University Center of Hermínio Ometto Foundation (FHO) California State University |
dc.contributor.author.fl_str_mv |
de Paiva, Marcela Britto Pegorin Brasil, Giovana Sant’Ana [UNESP] Chagas, Ana Laura Destro [UNESP] Macedo, Ana Paula Ramos, Júnia Issa, João Paulo Mardegan Gangrade, Ankit Floriano, Juliana Ferreira [UNESP] Caetano, Guilherme Ferreira Li, Bingbing Farhadi, Neda Mandal, Kalpana Dokmeci, Mehmet Remzi Jucaud, Vadim Herculano, Rondinelli Donizetti [UNESP] Shimano, Antonio Carlos |
description |
Due to the difficulty of consolidation, the risk of fractures, and the high cost of therapy, the orthopedic field views the restoration of bony defects or post-traumatic diseases as a significant issue. In recent years, efforts have been made toward guided bone regeneration as a viable solution. In this study, natural rubber latex (NRL) and latex–collagen (NRL-COL) membranes were developed and characterized, and their effects on bone regeneration in rat tibial lesions were assessed. According to the physicochemical findings, the hydrolyzed COL was effectively incorporated into the NRL membrane to produce a more robust membrane (348% increase in stress) and showed good biocompatibility for human dermal fibroblast cells and in hemolytic activity assay. According to bone analysis using computed microtomography, a substantial difference in bone volume (BV) was found among NRL, control (CONT), and NRL-COL. NRL-COL exhibited the greatest value for the bone volume/total volume ratio (BV/TV). The presence of NRL and COL was significant for the analysis of BV and BV/TV, respectively. All the experimental groups demonstrated a higher bone quality and fracture resistance, as shown by the mechanical properties of the bone. In the COL group, both neoformation of bone and total collagen levels were greater, indicating that treated defects had been closed and mature bone cells were present. Due to its angiogenic properties and the low cost of NRL membrane production, the NRL-COL membrane could be a good substitute for upcoming bone regeneration applications. Graphical Abstract: [Figure not available: see fulltext.] |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:59:58Z 2023-07-29T13:59:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10853-022-08009-7 Journal of Materials Science, v. 57, n. 48, p. 22019-22041, 2022. 1573-4803 0022-2461 http://hdl.handle.net/11449/249012 10.1007/s10853-022-08009-7 2-s2.0-85143614000 |
url |
http://dx.doi.org/10.1007/s10853-022-08009-7 http://hdl.handle.net/11449/249012 |
identifier_str_mv |
Journal of Materials Science, v. 57, n. 48, p. 22019-22041, 2022. 1573-4803 0022-2461 10.1007/s10853-022-08009-7 2-s2.0-85143614000 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Journal of Materials Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22019-22041 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128131973775360 |