Structural associations between organelle membranes in nectary parenchyma cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00425-018-2844-7 http://hdl.handle.net/11449/175753 |
Resumo: | Main conclusion: The close association between membranes and organelles, and the intense chloroplast remodeling in parenchyma cells of extrafloral nectaries occurred only at the secretion time and suggest a relationship with the nectar secretion. Associations between membranes and organelles have been well documented in different tissues and cells of plants, but poorly explored in secretory cells. Here, we described the close physical juxtaposition between membranes and organelles, mainly with chloroplasts, in parenchyma cells of Citharexylum myrianthum (Verbenaeceae) extrafloral nectaries under transmission electron microscopy, using conventional and microwave fixation. At the time of nectar secretion, nectary parenchyma cells exhibit a multitude of different organelle and membrane associations as mitochondria–mitochondria, mitochondria–endoplasmic reticulum, mitochondria–chloroplast, chloroplast–nuclear envelope, mitochondria–nuclear envelope, chloroplast–plasmalemma, chloroplast–chloroplast, chloroplast–tonoplast, chloroplast–peroxisome, and mitochondria–peroxisome. These associations were visualized as amorphous electron-dense material, a network of dense fibrillar material and/or dense bridges. Chloroplasts exhibited protrusions variable in shape and extension, which bring them closer to each other and to plasmalemma, tonoplast, and nuclear envelope. Parenchyma cells in the pre- and post-secretory stages did not exhibit any association or juxtaposition of membranes and organelles, and chloroplast protrusions were absent. Chloroplasts had peripheral reticulum that was more developed in the secretory stage. We propose that such subcellular phenomena during the time of nectar secretion optimize the movement of signaling molecules and the exchange of metabolites. Our results open new avenues on the potential mechanisms of organelle contact in parenchyma nectary cells, and reveal new attributes of the secretory cells on the subcellular level. |
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Structural associations between organelle membranes in nectary parenchyma cellsChloroplast remodelingMembrane associationNectaryOrganelle juxtapositionPlastidial protrusionsMain conclusion: The close association between membranes and organelles, and the intense chloroplast remodeling in parenchyma cells of extrafloral nectaries occurred only at the secretion time and suggest a relationship with the nectar secretion. Associations between membranes and organelles have been well documented in different tissues and cells of plants, but poorly explored in secretory cells. Here, we described the close physical juxtaposition between membranes and organelles, mainly with chloroplasts, in parenchyma cells of Citharexylum myrianthum (Verbenaeceae) extrafloral nectaries under transmission electron microscopy, using conventional and microwave fixation. At the time of nectar secretion, nectary parenchyma cells exhibit a multitude of different organelle and membrane associations as mitochondria–mitochondria, mitochondria–endoplasmic reticulum, mitochondria–chloroplast, chloroplast–nuclear envelope, mitochondria–nuclear envelope, chloroplast–plasmalemma, chloroplast–chloroplast, chloroplast–tonoplast, chloroplast–peroxisome, and mitochondria–peroxisome. These associations were visualized as amorphous electron-dense material, a network of dense fibrillar material and/or dense bridges. Chloroplasts exhibited protrusions variable in shape and extension, which bring them closer to each other and to plasmalemma, tonoplast, and nuclear envelope. Parenchyma cells in the pre- and post-secretory stages did not exhibit any association or juxtaposition of membranes and organelles, and chloroplast protrusions were absent. Chloroplasts had peripheral reticulum that was more developed in the secretory stage. We propose that such subcellular phenomena during the time of nectar secretion optimize the movement of signaling molecules and the exchange of metabolites. Our results open new avenues on the potential mechanisms of organelle contact in parenchyma nectary cells, and reveal new attributes of the secretory cells on the subcellular level.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Botany Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP)Center of Electron Microscopy (CME) Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP)Department of Botany Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP)Center of Electron Microscopy (CME) Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP)CNPq: 470649/2008-9Universidade Estadual Paulista (Unesp)Machado, Silvia Rodrigues [UNESP]Gregório, Elisa A. [UNESP]Rodrigues, Tatiane M. [UNESP]2018-12-11T17:17:22Z2018-12-11T17:17:22Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1067-1076application/pdfhttp://dx.doi.org/10.1007/s00425-018-2844-7Planta, v. 247, n. 5, p. 1067-1076, 2018.1432-20480032-0935http://hdl.handle.net/11449/17575310.1007/s00425-018-2844-72-s2.0-850406440362-s2.0-85040644036.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlanta1,5081,508info:eu-repo/semantics/openAccess2023-11-20T06:14:52Zoai:repositorio.unesp.br:11449/175753Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:15:47.242691Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Structural associations between organelle membranes in nectary parenchyma cells |
title |
Structural associations between organelle membranes in nectary parenchyma cells |
spellingShingle |
Structural associations between organelle membranes in nectary parenchyma cells Machado, Silvia Rodrigues [UNESP] Chloroplast remodeling Membrane association Nectary Organelle juxtaposition Plastidial protrusions |
title_short |
Structural associations between organelle membranes in nectary parenchyma cells |
title_full |
Structural associations between organelle membranes in nectary parenchyma cells |
title_fullStr |
Structural associations between organelle membranes in nectary parenchyma cells |
title_full_unstemmed |
Structural associations between organelle membranes in nectary parenchyma cells |
title_sort |
Structural associations between organelle membranes in nectary parenchyma cells |
author |
Machado, Silvia Rodrigues [UNESP] |
author_facet |
Machado, Silvia Rodrigues [UNESP] Gregório, Elisa A. [UNESP] Rodrigues, Tatiane M. [UNESP] |
author_role |
author |
author2 |
Gregório, Elisa A. [UNESP] Rodrigues, Tatiane M. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Machado, Silvia Rodrigues [UNESP] Gregório, Elisa A. [UNESP] Rodrigues, Tatiane M. [UNESP] |
dc.subject.por.fl_str_mv |
Chloroplast remodeling Membrane association Nectary Organelle juxtaposition Plastidial protrusions |
topic |
Chloroplast remodeling Membrane association Nectary Organelle juxtaposition Plastidial protrusions |
description |
Main conclusion: The close association between membranes and organelles, and the intense chloroplast remodeling in parenchyma cells of extrafloral nectaries occurred only at the secretion time and suggest a relationship with the nectar secretion. Associations between membranes and organelles have been well documented in different tissues and cells of plants, but poorly explored in secretory cells. Here, we described the close physical juxtaposition between membranes and organelles, mainly with chloroplasts, in parenchyma cells of Citharexylum myrianthum (Verbenaeceae) extrafloral nectaries under transmission electron microscopy, using conventional and microwave fixation. At the time of nectar secretion, nectary parenchyma cells exhibit a multitude of different organelle and membrane associations as mitochondria–mitochondria, mitochondria–endoplasmic reticulum, mitochondria–chloroplast, chloroplast–nuclear envelope, mitochondria–nuclear envelope, chloroplast–plasmalemma, chloroplast–chloroplast, chloroplast–tonoplast, chloroplast–peroxisome, and mitochondria–peroxisome. These associations were visualized as amorphous electron-dense material, a network of dense fibrillar material and/or dense bridges. Chloroplasts exhibited protrusions variable in shape and extension, which bring them closer to each other and to plasmalemma, tonoplast, and nuclear envelope. Parenchyma cells in the pre- and post-secretory stages did not exhibit any association or juxtaposition of membranes and organelles, and chloroplast protrusions were absent. Chloroplasts had peripheral reticulum that was more developed in the secretory stage. We propose that such subcellular phenomena during the time of nectar secretion optimize the movement of signaling molecules and the exchange of metabolites. Our results open new avenues on the potential mechanisms of organelle contact in parenchyma nectary cells, and reveal new attributes of the secretory cells on the subcellular level. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:22Z 2018-12-11T17:17:22Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00425-018-2844-7 Planta, v. 247, n. 5, p. 1067-1076, 2018. 1432-2048 0032-0935 http://hdl.handle.net/11449/175753 10.1007/s00425-018-2844-7 2-s2.0-85040644036 2-s2.0-85040644036.pdf |
url |
http://dx.doi.org/10.1007/s00425-018-2844-7 http://hdl.handle.net/11449/175753 |
identifier_str_mv |
Planta, v. 247, n. 5, p. 1067-1076, 2018. 1432-2048 0032-0935 10.1007/s00425-018-2844-7 2-s2.0-85040644036 2-s2.0-85040644036.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Planta 1,508 1,508 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1067-1076 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128912636510208 |